PCS1 Eukaryotic aspartyl protease family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PCS1
Official Full Name: Eukaryotic aspartyl protease family protein
Primary source: TAIR:AT5G02190
Locus tag: AT5G02190
See related: Araport:AT5G02190
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA ASPARTIC PROTEASE 38; ATASP38; EMB24; EMBRYO DEFECTIVE 24; PHYTOCHELATIN SYNTHASE 1; PROMOTION OF CELL SURVIVAL 1; T7H20.240; T7H20_240
Summary: encodes an aspartic protease, has an important role in determining cell fate during embryonic development and in reproduction processes. The loss-of-function mutation of PCS1 causes degeneration of both male and female gametophytes and excessive cell death of developing embryos during torpedo stage.
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Genomic context

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See PCS1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 1

Sequence:: Chromosome: 5; NC_003076.8 (435168..437018)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis transcription factor WRKY45 confers cadmium tolerance via activating PCS1 and PCS2 expression.
Title: Arabidopsis transcription factor WRKY45 confers cadmium tolerance via activating PCS1 and PCS2 expression.
C-terminal parts involved in arsenite- [As(III)] dependent activation of AtPCS1, the primary Arabidopsis phytochelatin synthase, were identified.
Title: Identification of C-terminal Regions in Arabidopsis thaliana Phytochelatin Synthase 1 Specifically Involved in Activation by Arsenite.
The heterologous expression of AtPCS1 in tobacco plants exposed to arsenic plus cadmium enhances phytochelatin levels, root As/Cd accumulation and pollutants detoxification, but does not prevent root cyto-histological damages.
Title: Overexpression of AtPCS1 in tobacco increases arsenic and arsenic plus cadmium accumulation and detoxification.
Cadmium and phytochelatin levels were determined in protoplasts and vacuoles isolated from leaves of Nicotiana tabacum expressing either of two phytochelatin synthase genes, AtPCS1 and CePCS.
Title: The role of subcellular distribution of cadmium and phytochelatins in the generation of distinct phenotypes of AtPCS1- and CePCS3-expressing tobacco.
contributes specifically to the cytosolic turnover of glutathione conjugates
Title: Cytosolic action of phytochelatin synthase.
Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site.
Title: Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site.
increase in arsenic tolerance in transformed tobacco plants
Title: Arsenic response of AtPCS1- and CePCS-expressing plants - effects of external As(V) concentration on As-accumulation pattern and NPT metabolism.
results enable us to propose an advanced model of PC synthesis, describing substrate specificity, heavy metal requirement, and the active site in the PCS1 enzyme
Title: Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay.
PROMOTION OF CELL SURVIVAL 1 (PCS1) gene in Arabidopsis, which encodes an aspartic protease, has an important role in determining the fate of cells in embryonic development and in reproduction processes [PCS1]
Title: An Arabidopsis aspartic protease functions as an anti-cell-death component in reproduction and embryogenesis.

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General gene information

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NM_120297.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables aspartic-type endopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within embryo development ending in seed dormancy	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Eukaryotic aspartyl protease family protein

NP_195839.1

PROMOTION OF CELL SURVIVAL 1 (PCS1); CONTAINS InterPro DOMAIN/s: Peptidase aspartic (InterPro:IPR021109), Peptidase aspartic, catalytic (InterPro:IPR009007), Peptidase A1 (InterPro:IPR001461), Peptidase aspartic, active site (InterPro:IPR001969); BEST Arabidopsis thaliana protein match is: Eukaryotic aspartyl protease family protein (TAIR:AT2G39710.1); Has 2066 Blast hits to 2053 proteins in 143 species: Archae - 0; Bacteria - 0; Metazoa - 95; Fungi - 96; Plants - 1822; Viruses - 0; Other Eukaryotes - 53 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

435168..437018

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_120297.4 → NP_195839.1 Eukaryotic aspartyl protease family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_195839.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LZL3

UniProtKB/TrEMBL

A0A5S9Y0M4

Conserved Domains (2) summary

cd05476
Location:82 → 442

pepsin_A_like_plant; Chroloplast Nucleoids DNA-binding Protease and Nucellin, pepsin-like aspartic proteases from plants

pfam00026
Location:82 → 440

Asp; Eukaryotic aspartyl protease