Ago2 argonaute RISC catalytic component 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ago2provided by RGD
Official Full Name: argonaute RISC catalytic component 2provided by RGD
Primary source: RGD:621255
See related: EnsemblRapid:ENSRNOG00000008533 AllianceGenome:RGD:621255
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Eif2c2; eIF2C 2
Summary: Enables Hsp70 protein binding activity; Hsp90 protein binding activity; and RISC complex binding activity. Involved in several processes, including regulation of macromolecule biosynthetic process; response to cocaine; and response to morphine. Located in several cellular components, including Golgi apparatus; cytoplasmic ribonucleoprotein granule; and dendrite. Part of RISC complex. Biomarker of morphine dependence and withdrawal disorder. Human ortholog(s) of this gene implicated in alcohol dependence and glaucoma. Orthologous to human AGO2 (argonaute RISC catalytic component 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Spleen (RPKM 251.5), Muscle (RPKM 174.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q34

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (106907209..106994124, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (105018202..105105118, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (114339607..114377277, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LncRNA CASC7 inhibits myocardial apoptosis in myocardial ischemia-reperfusion rats by regulating MiR-21 expression.
Title: LncRNA CASC7 inhibits myocardial apoptosis in myocardial ischemia-reperfusion rats by regulating MiR-21 expression.
Nrp-1 Mediated Plasmatic Ago2 Binding miR-21a-3p Internalization: A Novel Mechanism for miR-21a-3p Accumulation in Renal Tubular Epithelial Cells during Sepsis.
Title: Nrp-1 Mediated Plasmatic Ago2 Binding miR-21a-3p Internalization: A Novel Mechanism for miR-21a-3p Accumulation in Renal Tubular Epithelial Cells during Sepsis.
The partial loss-of-function of AGO2 in mature brown adipocytes is sufficient to lead to a diminished expression of UCP1 associated to a decreased uncoupled respiration. The downregulation of AGO2 in mature brown adipocytes induces a phenotype compatible with decreased thermogenic activity.
Title: Argonaute-2 is associated to brown adipose tissue activation.
Using rat hippocampal neurons, this study reports that dendritic Ago2 is down-regulated by the proteasome upon NMDA receptor activation.
Title: NMDA receptor-dependent dephosphorylation of serine 387 in Argonaute 2 increases its degradation and affects dendritic spine density and maturation.
Knockdown (KD) of AUF1 resulted in (1) transient upregulation of the mRNA level as well as an advancement of translation onset time (TOT) from 6 to 5 h post-serum addition, (2) loss of miRNA loading on AGO1 and AGO2 and (3) reduction in the level of AGO-1 and AGO-2 bound mRNA.
Title: Posttranscriptional regulation of cyclin D1 by ARE-binding proteins AUF1 and HuR in cycling myoblasts.
Neuronal differentiation activates the p38 signaling necessary for the phosphorylation of Ago2.
Title: Phosphorylation of Ago2 and Subsequent Inactivation of let-7a RNP-Specific MicroRNAs Control Differentiation of Mammalian Sympathetic Neurons.
In rat spermatogenic cells AGO2 colocalizes with the lysosomal membrane protein LAMP2 in small vesicles that contact and surround chromatoid bodies.
Title: Argonaute2 Protein in Rat Spermatogenic Cells Is Localized to Nuage Structures and LAMP2-Positive Vesicles Surrounding Chromatoid Bodies.
AGO1 and AGO2 play non-equivalent roles of in mRNA turnover and translation of Ccnd1 mRNA.
Title: Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA.
Target mRNAs induce tailing and trimming on Ago2-loaded miRNAs.
Title: Potent degradation of neuronal miRNAs induced by highly complementary targets.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables Hsp70 protein binding	IDA Inferred from Direct Assay more info	PubMed
enables Hsp90 protein binding	IDA Inferred from Direct Assay more info	PubMed
enables RISC complex binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA 7-methylguanosine cap binding	IEA Inferred from Electronic Annotation more info
enables RNA 7-methylguanosine cap binding	ISO Inferred from Sequence Orthology more info
enables RNA 7-methylguanosine cap binding	ISS Inferred from Sequence or Structural Similarity more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II complex binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II complex binding	ISO Inferred from Sequence Orthology more info
enables core promoter sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables core promoter sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables double-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	IEA Inferred from Electronic Annotation more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	IEA Inferred from Electronic Annotation more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	ISS Inferred from Sequence or Structural Similarity more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR AU-rich region binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR AU-rich region binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA cap binding	IEA Inferred from Electronic Annotation more info
enables mRNA cap binding	ISO Inferred from Sequence Orthology more info
enables mRNA cap binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IEA Inferred from Electronic Annotation more info
contributes_to siRNA binding	ISO Inferred from Sequence Orthology more info
enables siRNA binding	ISO Inferred from Sequence Orthology more info
enables siRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in P-body assembly	IEA Inferred from Electronic Annotation more info
involved_in P-body assembly	ISO Inferred from Sequence Orthology more info
involved_in RISC complex assembly	IEA Inferred from Electronic Annotation more info
involved_in RISC complex assembly	ISO Inferred from Sequence Orthology more info
involved_in RNA secondary structure unwinding	IEA Inferred from Electronic Annotation more info
involved_in RNA secondary structure unwinding	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to calcium ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in miRNA metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within miRNA metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	IEA Inferred from Electronic Annotation more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	IEA Inferred from Electronic Annotation more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of amyloid precursor protein biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translational initiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translational initiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translational initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of post-transcriptional gene silencing by RNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of trophoblast cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of trophoblast cell migration	ISO Inferred from Sequence Orthology more info
involved_in post-embryonic development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within post-embryonic development	ISO Inferred from Sequence Orthology more info
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in pre-miRNA processing	IEA Inferred from Electronic Annotation more info
involved_in pre-miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in pre-miRNA processing	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of mRNA stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse maturation	IEA Inferred from Electronic Annotation more info
involved_in regulation of synapse maturation	ISO Inferred from Sequence Orthology more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	ISO Inferred from Sequence Orthology more info
involved_in regulatory ncRNA-mediated gene silencing	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to cocaine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to morphine	IEP Inferred from Expression Pattern more info	PubMed
involved_in siRNA processing	IEA Inferred from Electronic Annotation more info
involved_in siRNA processing	ISO Inferred from Sequence Orthology more info
involved_in siRNA-mediated gene silencing by mRNA destabilization	IEA Inferred from Electronic Annotation more info
involved_in siRNA-mediated gene silencing by mRNA destabilization	ISO Inferred from Sequence Orthology more info
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IEA Inferred from Electronic Annotation more info
located_in P-body	ISO Inferred from Sequence Orthology more info
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	IEA Inferred from Electronic Annotation more info
part_of RISC complex	ISO Inferred from Sequence Orthology more info
part_of RISC complex	ISS Inferred from Sequence or Structural Similarity more info
part_of RISC-loading complex	IEA Inferred from Electronic Annotation more info
part_of RISC-loading complex	ISO Inferred from Sequence Orthology more info
part_of RISC-loading complex	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatoid body	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IEA Inferred from Electronic Annotation more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in pi-body	IDA Inferred from Direct Assay more info	PubMed
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein argonaute-2

Names: argonaute 2, RISC catalytic component; argonaute2; eukaryotic translation initiation factor 2C 2; golgi ER protein 95 kDa

NP_001395847.1

EC 3.1.26.n2

XP_038936149.1

EC 3.1.26.n2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001408918.2 → NP_001395847.1 protein argonaute-2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

Q9QZ81

UniProtKB/TrEMBL

F1LRP7

Related

ENSRNOP00000011898.4, ENSRNOT00000011898.6

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

106907209..106994124 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039080221.2 → XP_038936149.1 protein argonaute-2 isoform X1

UniProtKB/Swiss-Prot

Q9QZ81

Related

ENSRNOP00000095405.2, ENSRNOT00000120018.2

Conserved Domains (4) summary

cd02846
Location:181 → 301

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:346 → 771

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:130 → 180

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:46 → 120

ArgoN; N-terminal domain of argonaute