U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    KEG protein kinases;ubiquitin-protein ligase [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 831197, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    KEG
    Official Full Name
    protein kinases;ubiquitin-protein ligase
    Primary source
    TAIR:AT5G13530
    Locus tag
    AT5G13530
    See related
    Araport:AT5G13530
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    KEEP ON GOING; T6I14.60; T6I14_60
    Summary
    Encodes KEEP ON GOING (KEG), a RING E3 ligase involved in abscisic acid signaling. KEG is essential for Arabidopsis growth and development. ABA promotes KEG degradation via the ubiquitin dependent 26S proteasome pathway.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See KEG in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    16
    Sequence:
    Chromosome: 5; NC_003076.8 (4345202..4354723)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene Ribosomal protein L10 family protein Neighboring gene peptidase M1 family protein Neighboring gene miscRNA Neighboring gene sulfate transporter 4.1

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. The KEEP ON GOING protein of Arabidopsis regulates intracellular protein trafficking and is degraded during fungal infection. Gu Y, et al. Plant Cell, 2012 Nov. PMID 23192225, Free PMC Article
    2. Cytoplasmic degradation of the Arabidopsis transcription factor abscisic acid insensitive 5 is mediated by the RING-type E3 ligase KEEP ON GOING. Liu H, et al. J Biol Chem, 2013 Jul 12. PMID 23720747, Free PMC Article
    3. Arabidopsis CIPK26 interacts with KEG, components of the ABA signalling network and is degraded by the ubiquitin-proteasome system. Lyzenga WJ, et al. J Exp Bot, 2013 Jul. PMID 23658427, Free PMC Article
    4. The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles. Gu Y, et al. Plant Physiol, 2011 Apr. PMID 21343429, Free PMC Article
    5. Abscisic acid increases Arabidopsis ABI5 transcription factor levels by promoting KEG E3 ligase self-ubiquitination and proteasomal degradation. Liu H, et al. Plant Cell, 2010 Aug. PMID 20682837, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Arabidopsis E3 ligase KEG associates with and ubiquitinates MKK4 and MKK5 to regulate plant immunity.
      Title: Arabidopsis E3 ligase KEG associates with and ubiquitinates MKK4 and MKK5 to regulate plant immunity.
    2. KEG is capable of attaching ubiquitin to FDH in in vitro assays and the turnover of FDH was increased when co-expressed with a functional KEG in planta, suggesting that KEG contributes to FDH degradation.
      Title: Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.
    3. KEG is an important factor in plant hormone signaling and a positive regulator of JAZ12 stability.
      Title: The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.
    4. ABF1 and ABF3 abundance is affected by ABA and KEG
      Title: ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3.
    5. CIPK26(AT5G21326) interacts with KEG is degraded by the ubiquitin-proteasome system.
      Title: Arabidopsis CIPK26 interacts with KEG, components of the ABA signalling network and is degraded by the ubiquitin-proteasome system.
    6. Data indicate that KEEP ON GOING (KEG) E3 interacts with abscisic acid insensitive 5 (ABI5) in the cytoplasm.
      Title: Cytoplasmic degradation of the Arabidopsis transcription factor abscisic acid insensitive 5 is mediated by the RING-type E3 ligase KEEP ON GOING.
    7. KEG is a key component of trans-Golgi network and early endosomes (TGN/EE) that regulates post-Golgi trafficking events, including vacuole biogenesis, targeting of membrane-associated proteins to the vacuole, and secretion of apoplastic proteins.
      Title: The KEEP ON GOING protein of Arabidopsis regulates intracellular protein trafficking and is degraded during fungal infection.
    8. EDR1 and KEG function together to regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network/early endosome vesicles during stress responses.
      Title: The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles.
    9. Overexpression of KEG renders plant insensitive to abscisic acid and salt. Abscisic acid promotes KEG degradation via the ubiquitin-dependent 26S proteasome pathway.
      Title: Abscisic acid increases Arabidopsis ABI5 transcription factor levels by promoting KEG E3 ligase self-ubiquitination and proteasomal degradation.
    10. Loss-of-function mutations in KEG are seedling lethal and are hypersensitive to glucose and abscisic acid
      Title: Powdery mildew resistance conferred by loss of the ENHANCED DISEASE RESISTANCE1 protein kinase is suppressed by a missense mutation in KEEP ON GOING, a regulator of abscisic acid signaling.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_001203374.1

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in abscisic acid-activated signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within abscisic acid-activated signaling pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within abscisic acid-activated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in defense response IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within defense response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within developmental growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within endosomal transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within late endosome to vacuole transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of abscisic acid-activated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to abscisic acid IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within secretion by cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  
    located_in early endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in intracellular anatomical structure IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in trans-Golgi network IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    protein kinases;ubiquitin-protein ligase
    NP_001190303.1
    • KEEP ON GOING (KEG); CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, RING-type (InterPro:IPR001841), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Zinc finger, C3HC4 RING-type (InterPro:IPR018957), Ankyrin repeat-containing domain (InterPro:IPR020683), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (TAIR:AT4G32250.3).
    NP_196857.2
    • KEEP ON GOING (KEG); FUNCTIONS IN: ubiquitin-protein ligase activity, protein kinase activity; INVOLVED IN: abscisic acid mediated signaling pathway, response to abscisic acid stimulus, defense response, protein ubiquitination, developmental growth; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, RING-type (InterPro:IPR001841), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Ankyrin repeat-containing domain (InterPro:IPR020683), Zinc finger, C3HC4 RING-type (InterPro:IPR018957), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (TAIR:AT4G32250.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      4345202..4354723
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_121356.3 → NP_196857.2  protein kinases;ubiquitin-protein ligase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_196857.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q27YP2, Q9FY47, Q9FY48
      UniProtKB/TrEMBL
      A0A178URJ0, A0A654G0V7
      Conserved Domains (6) summary
      smart00220
      Location:138 → 424
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      PHA03095
      Location:517 → 857
      PHA03095; ankyrin-like protein; Provisional
      COG0666
      Location:427 → 571
      ANKYR; Ankyrin repeat [Signal transduction mechanisms]
      sd00045
      Location:688 → 722
      ANK; ANK repeat [structural motif]
      cd16568
      Location:9 → 56
      RING-HC_ScPSH1_like; RING finger, HC subclass, found in Saccharomyces cerevisiae POB3/SPT16 histone-associated protein 1 (ScPSH1), Arabidopsis thaliana Protein KEEP ON GOING (AtKEG) and similar proteins
      pfam18346
      Location:1560 → 1617
      SH3_15; Mind bomb SH3 repeat domain

    2. NM_001203374.1 → NP_001190303.1  protein kinases;ubiquitin-protein ligase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190303.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178UP88, F4K3Z6
      Conserved Domains (7) summary
      smart00220
      Location:138 → 424
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd00204
      Location:506 → 633
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd14014
      Location:138 → 432
      STKc_PknB_like; Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins
      pfam12796
      Location:515 → 610
      Ank_2; Ankyrin repeats (3 copies)
      pfam13445
      Location:10 → 53
      zf-RING_UBOX; RING-type zinc-finger
      pfam13857
      Location:599 → 654
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:688 → 721
      ANK; ANK repeat [structural motif]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9FY48.2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools