Tkt transketolase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tktprovided by MGI
Official Full Name: transketolaseprovided by MGI
Primary source: MGI:MGI:105992
See related: Ensembl:ENSMUSG00000021957 AllianceGenome:MGI:105992
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p68
Summary: This gene encodes an enzyme that binds magnesium and thiamine pyrophosphate and catalyzes the transfer of sugar phosphates to an aldose acceptor. This enzyme is a key component of the pentose phosphate pathway during glycolysis. It is significantly expressed in the cornea and may be involved in the cellular response against oxidative stress. Haploinsufficiency of this gene leads to decreased growth and reduction of adipose tissue. [provided by RefSeq, Dec 2013]
Expression: Broad expression in mammary gland adult (RPKM 585.4), adrenal adult (RPKM 535.9) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 B; 14 18.79 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (30271088..30296681)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (30549131..30574724)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway.
Title: HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway.
Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.
Title: Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.
Nuclear Tkt promotes ischemic heart failure via the cleaved Parp1/Aif axis.
Title: Nuclear Tkt promotes ischemic heart failure via the cleaved Parp1/Aif axis.
TKT maintains intestinal ATP production and inhibits apoptosis-induced colitis.
Title: TKT maintains intestinal ATP production and inhibits apoptosis-induced colitis.
Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.
Title: Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.
Zinc finger protein 746 overexpression suppresses TKT transcription via IRS-like motif in the TKT promoter.
Title: Identification of transketolase as a target of PARIS in substantia nigra.
decreased TK activity leads to pentose-phosphate pathway dysfunction and contributes to impaired hippocampal neurogenesis induced by thiamine deficiency
Title: Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

TKT (e-PCR)
- UniSTS:505187

Tkt (e-PCR), detects polymorphism
- UniSTS:507279

Tkt (e-PCR), detects polymorphism
- UniSTS:144636

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables carbohydrate binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables monosaccharide binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables thiamine pyrophosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables thiamine pyrophosphate binding	ISO Inferred from Sequence Orthology more info
enables transketolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transketolase activity	IDA Inferred from Direct Assay more info	PubMed
enables transketolase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in glyceraldehyde-3-phosphate biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in glyceraldehyde-3-phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in pentose-phosphate shunt	IDA Inferred from Direct Assay more info	PubMed
involved_in pentose-phosphate shunt	ISO Inferred from Sequence Orthology more info
involved_in pentose-phosphate shunt, non-oxidative branch	IBA Inferred from Biological aspect of Ancestor more info
involved_in pentose-phosphate shunt, non-oxidative branch	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pentose-phosphate shunt, non-oxidative branch	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in xylulose 5-phosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	HDA more info	PubMed
located_in nuclear body	IEA Inferred from Electronic Annotation more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: transketolase

Names: TK

NP_033414.1

EC 2.2.1.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009388.6 → NP_033414.1 transketolase

See identical proteins and their annotated locations for NP_033414.1

Status: REVIEWED

Source sequence(s)

AC154646, AI837706, AK030446, AK166763, BY276711

Consensus CDS

CCDS26894.1

UniProtKB/Swiss-Prot

P40142, Q3U7Y1, Q3UK62, Q545A1, Q9ESA0

UniProtKB/TrEMBL

A0A286YE28

Related

ENSMUSP00000022529.7, ENSMUST00000022529.8

Conserved Domains (4) summary

cd07033
Location:319 → 474

TPP_PYR_DXS_TK_like; Pyrimidine (PYR) binding domain of 1-deoxy-D-xylulose-5-phosphate synthase (DXS), transketolase (TK), and related proteins

PRK05899
Location:8 → 620

PRK05899; transketolase; Reviewed

cd02012
Location:20 → 271

TPP_TK; Thiamine pyrophosphate (TPP) family, Transketolase (TK) subfamily, TPP-binding module; TK catalyzes the transfer of a two-carbon unit from ketose phosphates to aldose phosphates. In heterotrophic organisms, TK provides a link between glycolysis and the ...

pfam02780
Location:492 → 612

Transketolase_C; Transketolase, C-terminal domain