nfi-1 Nuclear factor I family protein [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: nfi-1
Official Full Name: Nuclear factor I family protein
Primary source: WormBase:WBGene00003592
Locus tag: CELE_ZK1290.4
See related: AllianceGenome:WB:WBGene00003592
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; regulation of egg-laying behavior; and regulation of nematode pharyngeal pumping. Acts upstream of or within determination of adult lifespan. Predicted to be active in nucleus. Is expressed in several structures, including body wall musculature; egg-laying apparatus; intestine; neurons; and pharynx. Human ortholog(s) of this gene implicated in Marshall-Smith syndrome; NFIA-related disorder; and Sotos syndrome 2. Orthologous to several human genes including NFIA (nuclear factor I A). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See nfi-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 15

Sequence:: Chromosome: II; NC_003280.10 (7538180..7543007)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These experiments provide a foundation for understanding how NFI-1 is recruited to unexpectedly few in vivo sites to perform its developmental functions, despite a vast over-representation of its binding motif.
Title: DNA-binding specificity and in vivo targets of Caenorhabditis elegans nuclear factor I.
We propose some relationships between the pharyngeal pumping and lifespan phenotypes and potential mechanisms of nfi-1 function
Title: Lifespan extension and increased pumping rate accompany pharyngeal muscle-specific expression of nfi-1 in C. elegans.
The phenotype of nfi-1 null worms indicates that NFI functions in multiple developmental and behavioral systems in C. elegans, likely regulating genes that function in motility, egg-laying, pharyngeal pumping and lifespan maintenance.
Title: nfi-I affects behavior and life-span in C. elegans but is not essential for DNA replication or survival.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

STS2-157 (e-PCR)
- UniSTS:241826

ZK1290.4 (e-PCR)
- UniSTS:312483

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of multicellular organismal process	IEA Inferred from Electronic Annotation more info
involved_in regulation of nematode pharyngeal pumping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Nuclear factor I family protein

NP_001022505.1

Confirmed by transcript evidence

NP_001367579.1

Confirmed by transcript evidence

NP_001367580.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

7538180..7543007

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001027334.3 → NP_001022505.1 Nuclear factor I family protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001022505.1

Status: REVIEWED

UniProtKB/TrEMBL

Q09631

Conserved Domains (2) summary

pfam03165
Location:112 → 215

MH1; MH1 domain

pfam10524
Location:50 → 90

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus
NM_001381499.3 → NP_001367579.1 Nuclear factor I family protein [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (2) summary

pfam10524
Location:33 → 70

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

pfam03165
Location:92 → 195

MH1; MH1 domain
NM_001381498.1 → NP_001367580.1 Nuclear factor I family protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q09631, Q5H9N2, Q5H9N3

Conserved Domains (2) summary

pfam10524
Location:69 → 106

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

pfam03165
Location:128 → 231

MH1; MH1 domain