aff-1 Cell fusion protein aff-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: aff-1
Official Full Name: Cell fusion protein aff-1
Primary source: WormBase:WBGene00016625
Locus tag: CELE_C44B7.3
See related: AllianceGenome:WB:WBGene00016625
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Involved in regulation of egg-laying behavior and syncytium formation by plasma membrane fusion. Located in plasma membrane. Is expressed in anchor cell; epithelial cell; neurons; and pharyngeal muscle cell. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See aff-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 12

Sequence:: Chromosome: II; NC_003280.10 (6860582..6863752, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the remodeling of transected dendritic trees by AFF-1-mediated self-fusion can be restored in old animals by daf-2 mutations, and can be differentially re-established by ectopic expression of the fusion protein AFF-1.
Title: The fusogen AFF-1 can rejuvenate the regenerative potential of adult dendritic trees by self-fusion.
AFF-1 is required for scission of basal endocytic compartments and for apically directed exocytosis to extend the apical membrane
Title: The AFF-1 exoplasmic fusogen is required for endocytic scission and seamless tube elongation.
EFF-1 and AFF-1 fusion proteins emerge as new players in neuronal arborization and maintenance of arbor connectivity following injury in Caenorhabditis elegans Our results demonstrate that there is a genetically determined multi-step pathway to repair broken dendrites in which EFF-1 and AFF-1 act on different steps of the pathway.
Title: Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.
AFF-1 formed supercomplexes on pseudoviruses; viruses carrying AFF-1 infected mammalian cells only when CeFFs were on target cell surface; FF proteins are part of ancient family of cellular fusogens that can promote fusion when expressed on viral particle
Title: Conserved eukaryotic fusogens can fuse viral envelopes to cells.
FOS-1, the Fos transcription factor ortholog that controls anchor-cell invasion during nematode development, is a specific activator of aff-1-mediated anchor-cell fusion.
Title: AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans.

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General gene information

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Markers

C44B7.3 (e-PCR)
- UniSTS:308763

STS2-152 (e-PCR)
- UniSTS:241821

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Process	Evidence Code	Pubs
involved_in regulation of egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytium formation by plasma membrane fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in syncytium formation by plasma membrane fusion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in cell-cell contact zone	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular organelle	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Cell fusion protein aff-1

NP_495402.3

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6860582..6863752 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063001.6 → NP_495402.3 Cell fusion protein aff-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495402.3

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EGL9

Conserved Domains (1) summary

pfam14884
Location:32 → 565

EFF-AFF; Type I membrane glycoproteins cell-cell fusogen