Txnrd3 thioredoxin reductase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Txnrd3provided by RGD
Official Full Name: thioredoxin reductase 3provided by RGD
Primary source: RGD:1308363
See related: AllianceGenome:RGD:1308363
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. There is evidence for two isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017]
Expression: Biased expression in Testes (RPKM 428.5), Heart (RPKM 64.1) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q34

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (123629768..123669713)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (122072548..122112493)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (121612332..121650543)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Gpx4, Selenov, and Txnrd3 Are Three Most Testis-Abundant Selenogenes Resistant to Dietary Selenium Concentrations and Actively Expressed During Reproductive Ages in Rats.
Title: Gpx4, Selenov, and Txnrd3 Are Three Most Testis-Abundant Selenogenes Resistant to Dietary Selenium Concentrations and Actively Expressed During Reproductive Ages in Rats.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables thioredoxin-disulfide reductase (NADPH) activity	IBA Inferred from Biological aspect of Ancestor more info
enables thioredoxin-disulfide reductase (NADPH) activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within blastocyst formation	ISO Inferred from Sequence Orthology more info
involved_in cell redox homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular oxidant detoxification	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glutathione metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to oxidative stress	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: thioredoxin reductase 3

NP_001100079.2

EC 1.8.1.9

NP_001171641.1

EC 1.8.1.9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001106609.2 → NP_001100079.2 thioredoxin reductase 3 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (1) contains two in-frame translation initiation codons: an upstream non-AUG (CUG) and a downstream AUG codon. The isoform (2) represented in this RefSeq results from the use of the AUG start codon. It has a shorter N-terminus compared to isoform 1.

Source sequence(s)

BF564749, BG380508, CB327915, CB585038, CK603679, DN936395, JAXUCZ010000004

UniProtKB/TrEMBL

A0A0G2K0G3

Conserved Domains (5) summary

cd03419
Location:39 → 120

GRX_GRXh_1_2_like; Glutaredoxin (GRX) family, GRX human class 1 and 2 (h_1_2)-like subfamily; composed of proteins similar to human GRXs, approximately 10 kDa in size, and proteins containing a GRX or GRX-like domain. GRX is a glutathione (GSH) dependent reductase, ...

TIGR01438
Location:127 → 615

TGR; thioredoxin and glutathione reductase selenoprotein

pfam00070
Location:308 → 384

Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase

pfam02852
Location:487 → 597

Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerization domain

cl21454
Location:126 → 158

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins
NM_001184712.1 → NP_001171641.1 thioredoxin reductase 3 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) contains two in-frame translation initiation codons: an upstream non-AUG (CUG) and a downstream AUG codon. The longer isoform (1) represented in this RefSeq results from the use of the CUG start codon.

Source sequence(s)

BF564749, BG380508, CB327915, CB585038, CK603679, DN936395, JAXUCZ010000004

UniProtKB/TrEMBL

A0A0G2K764

Conserved Domains (5) summary

cd03419
Location:76 → 157

GRX_GRXh_1_2_like; Glutaredoxin (GRX) family, GRX human class 1 and 2 (h_1_2)-like subfamily; composed of proteins similar to human GRXs, approximately 10 kDa in size, and proteins containing a GRX or GRX-like domain. GRX is a glutathione (GSH) dependent reductase, ...

TIGR01438
Location:164 → 652

TGR; thioredoxin and glutathione reductase selenoprotein

pfam00070
Location:345 → 421

Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase

pfam02852
Location:524 → 634

Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerization domain

cl21454
Location:163 → 195

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins