Dio1 iodothyronine deiodinase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dio1provided by RGD
Official Full Name: iodothyronine deiodinase 1provided by RGD
Primary source: RGD:2504
See related: EnsemblRapid:ENSRNOG00000061237 AllianceGenome:RGD:2504
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: 5DI; ITDI1; TXDI1
Summary: The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2016]
Expression: Biased expression in Kidney (RPKM 1336.0) and Liver (RPKM 709.8) See more
Orthologs: human mouse all
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Genomic context

Location:: 5q34

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (127303089..127319784, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (122074285..122090983, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (126894837..126911532, complement)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Adaptive Thermogenesis Driving Catch-Up Fat Is Associated With Increased Muscle Type 3 and Decreased Hepatic Type 1 Iodothyronine Deiodinase Activities: A Functional and Proteomic Study.
Title: Adaptive Thermogenesis Driving Catch-Up Fat Is Associated With Increased Muscle Type 3 and Decreased Hepatic Type 1 Iodothyronine Deiodinase Activities: A Functional and Proteomic Study.
Effect of Preeclampsia on Ultrastructure of Thyroid Gland, Hepatic Type 1 Iodothyronine Deiodinase, and Thyroid Hormone Levels in Rats.
Title: Effect of Preeclampsia on Ultrastructure of Thyroid Gland, Hepatic Type 1 Iodothyronine Deiodinase, and Thyroid Hormone Levels in Rats.
TGF-beta1 mediates cell proliferation and development of hepatocarcinogenesis by downregulating deiodinase 1 expression.", trans "La desregulacion de la deiodinasa 1, mediada por TGF-beta1, impulsa la proliferacion celular y el desarrollo de hepatocarcinogenesis.
Title: TGF-β1 mediates cell proliferation and development of hepatocarcinogenesis by downregulating deiodinase 1 expression.
estrogen deficiency impairs the induction of thyroid hormone activating enzyme D1 in the pituitary, and GH release by acute exercise. Also, acute D1 activation is essential for exercise-induced GH response
Title: Thyroid hormone and estrogen regulate exercise-induced growth hormone release.
Response of pituitary Dio1 and brown adipose tissue Dio2 is blunted in ovariectomized rats.
Title: Blunted response of pituitary type 1 and brown adipose tissue type 2 deiodinases to swimming training in ovariectomized rats.
The effects of androgen deprivation on thyroid homeostasis, reflected in DioI and DioII expression, are reported.
Title: Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity.
Type 1 and type 2 deiodinase may have distinct functions in white adipose tissue, regulating lipogenesis and lipolysis.
Title: Presence and regulation of D1 and D2 deiodinases in rat white adipose tissue.
The study provided first evidence on the reduction of liver RAR concentrations and 5'-DI activity in response to long-term voluntary wheel running.
Title: Changes in retinoic acid receptor status, 5'-deiodinase activity and neuroendocrine response to voluntary wheel running.
The results demonstrate that decreased liver D1 and BAT D2 might be involved in the decreased T(4)-to-T(3) conversion detected after an exercise session on the treadmill.
Title: The effect of acute exercise session on thyroid hormone economy in rats.
Epididymis expresses the highest 5'-deiodinase activity in the male reproductive system.
Title: Epididymis expresses the highest 5'-deiodinase activity in the male reproductive system: kinetic characterization, distribution, and hormonal regulation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dio1 (e-PCR), detects polymorphism
- UniSTS:142850

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables selenium binding	ISO Inferred from Sequence Orthology more info
enables thyroxine 5'-deiodinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables thyroxine 5'-deiodinase activity	IDA Inferred from Direct Assay more info	PubMed
enables thyroxine 5'-deiodinase activity	IEA Inferred from Electronic Annotation more info
enables thyroxine 5'-deiodinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in amino acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in amino acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in generation of precursor metabolites and energy	NAS Non-traceable Author Statement more info	PubMed
involved_in hormone biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in thyroid hormone metabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: type I iodothyronine deiodinase

Names: DIOI; deiodinase, iodothyronine, type I; thyroxine deiodinase, type I; type 1 DI; type-I 5'-deiodinase

NP_001311139.1

EC 1.21.99.4

NP_067685.5

EC 1.21.99.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001324210.1 → NP_001311139.1 type I iodothyronine deiodinase isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which causes a frameshift compared to variant 1. The resulting isoform (2) is shorter, with a distinct C-terminus compared to isoform 1.

Source sequence(s)

BC083557, JAXUCZ010000005

UniProtKB/Swiss-Prot

P24389
NM_021653.4 → NP_067685.5 type I iodothyronine deiodinase isoform 1

See identical proteins and their annotated locations for NP_067685.5

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

BC083557, JAXUCZ010000005

UniProtKB/Swiss-Prot

P24389

UniProtKB/TrEMBL

A0A0G2JXM4

Related

ENSRNOP00000070330.2, ENSRNOT00000091521.3

Conserved Domains (1) summary

cl00388
Location:8 → 247

Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold