Ttll8 tubulin tyrosine ligase-like family, member 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ttll8provided by MGI
Official Full Name: tubulin tyrosine ligase-like family, member 8provided by MGI
Primary source: MGI:MGI:1922902
See related: Ensembl:ENSMUSG00000022388 AllianceGenome:MGI:1922902
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4933401B17; 1700019P01Rik
Summary: Enables protein-glycine ligase activity, initiating. Involved in flagellated sperm motility and protein polyglycylation. Acts upstream of or within cilium assembly. Located in axoneme; microtubule cytoskeleton; and sperm flagellum. Orthologous to human TTLL8 (tubulin tyrosine ligase like 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 37.7) See more
Orthologs: human all
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Genomic context

Location:: 15 E3; 15 44.39 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (88773981..88839258, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (88889778..88955051, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
Title: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
The TTLL8 was required for stability and maintenance of ependymal cilia, whereas the polyglutamylase TTLL6 was necessary for coordinated beating behavior.
Title: Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia.
TTLL10 can strongly glycylate tubulin in a TTLL8-dependent manner. Furthermore, these two TTLL proteins can glycylate unidentified 170-, 110-, 75-, 40-, 35-, and 30-kDa acidic proteins.
Title: TTLL10 can perform tubulin glycylation when co-expressed with TTLL8.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (5) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein-glycine ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-glycine ligase activity, initiating	IBA Inferred from Biological aspect of Ancestor more info
enables protein-glycine ligase activity, initiating	IDA Inferred from Direct Assay more info	PubMed
enables protein-glycine ligase activity, initiating	IMP Inferred from Mutant Phenotype more info	PubMed
enables tubulin-glycine ligase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in axoneme assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cilium assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flagellated sperm motility	IBA Inferred from Biological aspect of Ancestor more info
involved_in flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyglycylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyglycylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in axoneme	IBA Inferred from Biological aspect of Ancestor more info
located_in axoneme	IDA Inferred from Direct Assay more info	PubMed
located_in cilium	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in sperm flagellum	IBA Inferred from Biological aspect of Ancestor more info
located_in sperm flagellum	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein monoglycylase TTLL8

Names: TTL domain protein; tubulin--tyrosine ligase-like protein 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357736.2 → NP_001344665.1 protein monoglycylase TTLL8 isoform 2

Status: VALIDATED

Source sequence(s)

AC119959

Conserved Domains (1) summary

pfam03133
Location:193 → 415

TTL; Tubulin-tyrosine ligase family
NM_001428803.1 → NP_001415732.1 protein monoglycylase TTLL8 isoform 3

Status: VALIDATED

Source sequence(s)

AC117700, AC119959
NM_001428811.1 → NP_001415740.1 protein monoglycylase TTLL8 isoform 3

Status: VALIDATED

Source sequence(s)

AC117700, AC119959
NM_172818.4 → NP_766406.3 protein monoglycylase TTLL8 isoform 1

Status: VALIDATED

Source sequence(s)

AC119959

Consensus CDS

CCDS27735.2

UniProtKB/Swiss-Prot

A4Q9F1, Q14B76, Q8C0N7

Related

ENSMUSP00000104996.2, ENSMUST00000109371.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

88773981..88839258 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036159403.1 → XP_036015296.1 protein monoglycylase TTLL8 isoform X3
XM_011245621.3 → XP_011243923.1 protein monoglycylase TTLL8 isoform X2

UniProtKB/Swiss-Prot

A4Q9F1, Q14B76, Q8C0N7

Conserved Domains (1) summary

pfam03133
Location:487 → 709

TTL; Tubulin-tyrosine ligase family
XM_006520959.5 → XP_006521022.1 protein monoglycylase TTLL8 isoform X4

See identical proteins and their annotated locations for XP_006521022.1

UniProtKB/TrEMBL

Q8C0V2

Conserved Domains (1) summary

pfam03133
Location:193 → 415

TTL; Tubulin-tyrosine ligase family
XM_006520955.4 → XP_006521018.1 protein monoglycylase TTLL8 isoform X1

UniProtKB/Swiss-Prot

A4Q9F1, Q14B76, Q8C0N7

Conserved Domains (1) summary

pfam03133
Location:487 → 709

TTL; Tubulin-tyrosine ligase family
XM_011245623.4 → XP_011243925.1 protein monoglycylase TTLL8 isoform X6
XM_011245622.4 → XP_011243924.1 protein monoglycylase TTLL8 isoform X5