Pfkfb1 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pfkfb1provided by RGD
Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1provided by RGD
Primary source: RGD:3307
See related: EnsemblRapid:ENSRNOG00000000165 AllianceGenome:RGD:3307
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PFRX; Pfkfb01
Summary: Enables several functions, including 6-phosphofructo-2-kinase activity; anion binding activity; and fructose-2,6-bisphosphate 2-phosphatase activity. Involved in several processes, including carbohydrate phosphorylation; positive regulation of phosphate metabolic process; and response to glucagon. Part of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex. Biomarker of hyperinsulinism. Orthologous to human PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Muscle (RPKM 119.2), Liver (RPKM 49.0) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq12

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (22936038..22989691)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (19508522..19562165)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (23092143..23145923, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that a major portion of intracellular glucose in cardiac myocytes is apportioned for ancillary biosynthetic reactions; PFKFB1 coordinates activities of pentose phosphate, hexosamine biosynthetic, and fatty acid/glycerolipid synthesis pathways by directly modulating glycolytic intermediate entry into auxiliary glucose metabolism pathways and by indirectly regulating mitochondrial cataplerosis.
Title: Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.
Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+).
Title: The mechanism of calcium-induced inhibition of muscle fructose 1,6-bisphosphatase and destabilization of glyconeogenic complex.
a new mRNA variant which are transcribed from a new Pfkfb1 gene promoter in fetal rat liver was characterized.
Title: Identification of a novel Pfkfb1 mRNA variant in rat fetal liver.
flexible kinase interaction mode exists, suggesting dimer conformation mediated coupling of hormonal and posttranslational enzyme regulation to the metabolic response in liver
Title: Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation.
Results describe structural differences between two isozymes of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver and testis.
Title: Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Submit: New GeneRIF Correction

General gene information

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Markers

DXArb5 (e-PCR)
- UniSTS:240130

DXWox4 (e-PCR)
- UniSTS:118081

DXMgh5 (e-PCR)
- UniSTS:119407

RH130115 (e-PCR)
- UniSTS:213403

RH94813 (e-PCR)
- UniSTS:120059

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 6-phosphofructo-2-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructo-2-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 6-phosphofructo-2-kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables fructose-2,6-bisphosphate 2-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	ISO Inferred from Sequence Orthology more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables fructose-6-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in animal organ regeneration	IEP Inferred from Expression Pattern more info	PubMed
involved_in carbohydrate phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in carbohydrate phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in fructose 2,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 2,6-bisphosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fructose 2,6-bisphosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fructose 2,6-bisphosphate metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in fructose 2,6-bisphosphate metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in fructose metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of glycolytic process through fructose-6-phosphate	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glucokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of glycolytic process through fructose-6-phosphate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucagon	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to insulin	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to starvation	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex	IDA Inferred from Direct Assay more info	PubMed
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex	ISO Inferred from Sequence Orthology more info
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info

General protein information

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Preferred Names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1

Names: 6-PF2-K/Fru-2,6-P2ase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; PFK2/FBPase2

NP_001257992.1

EC 2.7.1.105

EC 3.1.3.46

NP_001257993.1

EC 2.7.1.105

EC 3.1.3.46

NP_036753.4

EC 2.7.1.105

EC 3.1.3.46

XP_006256852.1

EC 2.7.1.105

EC 3.1.3.46

XP_006256853.1

EC 2.7.1.105

EC 3.1.3.46

XP_008771345.1

EC 2.7.1.105

EC 3.1.3.46

XP_008771346.1

EC 2.7.1.105

EC 3.1.3.46

XP_038955409.1

EC 2.7.1.105

EC 3.1.3.46

XP_038955411.1

EC 2.7.1.105

EC 3.1.3.46

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271063.1 → NP_001257992.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

See identical proteins and their annotated locations for NP_001257992.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A0A0H2UH89, A6KL78, C9DRP4

Related

ENSRNOP00000000178.6, ENSRNOT00000000178.9

Conserved Domains (2) summary

pfam00300
Location:252 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:28 → 249

6PF2K; 6-phosphofructo-2-kinase
NM_001271064.1 → NP_001257993.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 3

See identical proteins and their annotated locations for NP_001257993.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1.

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A0A0G2K0S8, C9DRP4

Related

ENSRNOP00000071567.1, ENSRNOT00000081239.3

Conserved Domains (2) summary

pfam01591
Location:31 → 207

6PF2K; 6-phosphofructo-2-kinase

pfam00300
Location:210 → 396

His_Phos_1; Histidine phosphatase superfamily (branch 1)
NM_012621.4 → NP_036753.4 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 1

See identical proteins and their annotated locations for NP_036753.4

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

Y00702

UniProtKB/Swiss-Prot

P07953, P16119

UniProtKB/TrEMBL

A6KL80, C9DRP4

Related

ENSRNOP00000105342.1, ENSRNOT00000174108.1

Conserved Domains (3) summary

pfam00300
Location:254 → 437

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 251

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:44 → 198

AAA_33; AAA domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

22936038..22989691

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008773124.4 → XP_008771346.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X1

See identical proteins and their annotated locations for XP_008771346.1

UniProtKB/TrEMBL

A0A0H2UH89, A6KL78, C9DRP4

Conserved Domains (2) summary

pfam00300
Location:252 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:28 → 249

6PF2K; 6-phosphofructo-2-kinase
XM_008773123.3 → XP_008771345.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X1

See identical proteins and their annotated locations for XP_008771345.1

UniProtKB/TrEMBL

A0A0H2UH89, A6KL78, C9DRP4

Conserved Domains (2) summary

pfam00300
Location:252 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:28 → 249

6PF2K; 6-phosphofructo-2-kinase
XM_039099483.2 → XP_038955411.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X5

UniProtKB/TrEMBL

Q5Y297

Conserved Domains (1) summary

pfam01591
Location:30 → 216

6PF2K; 6-phosphofructo-2-kinase
XM_039099481.2 → XP_038955409.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X4

Conserved Domains (2) summary

pfam00300
Location:254 → 366

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 251

6PF2K; 6-phosphofructo-2-kinase
XM_006256790.5 → XP_006256852.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X2

UniProtKB/TrEMBL

C9DRP4

Related

ENSRNOP00000035212.2, ENSRNOT00000033656.4

Conserved Domains (2) summary

pfam00300
Location:212 → 395

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:34 → 209

6PF2K; 6-phosphofructo-2-kinase
XM_006256791.5 → XP_006256853.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X3

UniProtKB/TrEMBL

C9DRP4

Conserved Domains (3) summary

pfam00300
Location:211 → 394

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:1 → 208

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:1 → 155

AAA_33; AAA domain