POLH Y-family DNA polymerase H [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: POLH
Official Full Name: Y-family DNA polymerase H
Primary source: TAIR:AT5G44740
Locus tag: AT5G44740
See related: Araport:AT5G44740
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: K23L20.8; K23L20_8; Y-family DNA polymerase H
Summary: Y-family DNA polymerase. Catalyses translesion synthesis in response to UV damage. Functionally interacts with PCNA2. Has a ubiquitin binding motif.
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Genomic context

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See POLH in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 14

Sequence:: Chromosome: 5; NC_003076.8 (18047733..18051896, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cooperation among DNA translesion synthesis (TLS) polymerases (Poleta, Polzeta) and DNA-damage-activated protein kinases (ATR, ATM)
Title: Cooperative responses of DNA-damage-activated protein kinases ATR and ATM and DNA translesion polymerases to replication-blocking DNA damage in a stem-cell niche.
The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed.
Title: Role of AtPolζ, AtRev1 and AtPolη in γ ray-induced mutagenesis.
Arabidopsis thaliana disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode Translesion synthesis-type polymerases.
Title: Role of AtPolζ, AtRev1, and AtPolη in UV light-induced mutagenesis in Arabidopsis.
Results showed that crossing two lines homozygous for insertions in AtREV3 and AtPOLH and selfing F1 plants yielded non-Mendelian F2 genotype distributions: frequencies of +/++/+ and 1/1 2/2 progeny were only 0.42 and 0.25%.
Title: Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines.
Transgenic plants over-expressing POLH showed increased resistance to ultraviolet light.[POLH]
Title: Two translesion synthesis DNA polymerase genes, AtPOLH and AtREV1, are involved in development and UV light resistance in Arabidopsis.
AtPolH confers UV resistance by interacting with PNCA2 and catalyzing translesion synthesis. PCNA1 does not interact with AtPolH to restore UV resistance in deficient mutants.
Title: Arabidopsis thaliana Y-family DNA polymerase eta catalyses translesion synthesis and interacts functionally with PCNA2.
Evolutionary conservation of efficient T[CPD]T bypass by HsPoleta and AtPoleta may reflect a high degree of exposure of human skin and plants to solar UV-B radiation
Title: Biochemical evolution of DNA polymerase eta: properties of plant, human, and yeast proteins.
AtPolH is a Y-family DNA polymerase, homologous to Polymerase eta from yeast, human and other organisms. It is involved in DNA translesion synthesis. [AtPolH]
Title: Analysis of UV-induced mutation spectra in Escherichia coli by DNA polymerase eta from Arabidopsis thaliana.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DXS9848 (e-PCR)
- UniSTS:474256

Homology

Orthologs from OrthoDB

NM_001344572.1

Supported by ESTs:

NM_123841.5

Supported by ESTs:

NM_180799.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in UV protection	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to UV-B	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Y-family DNA polymerase H

NP_568638.2

Y-family DNA polymerase H (POLH); CONTAINS InterPro DOMAIN/s: DNA polymerase eta (InterPro:IPR017061), DNA-repair protein, UmuC-like (InterPro:IPR001126), DNA-repair protein, UmuC-like, N-terminal (InterPro:IPR017963); BEST Arabidopsis thaliana protein match is: DNA-directed DNA polymerases (TAIR:AT5G44750.1); Has 8088 Blast hits to 8015 proteins in 2292 species: Archae - 210; Bacteria - 5635; Metazoa - 500; Fungi - 401; Plants - 163; Viruses - 3; Other Eukaryotes - 1176 (source: NCBI BLink).

NP_851130.1

Y-family DNA polymerase H (POLH); FUNCTIONS IN: DNA-directed DNA polymerase activity; INVOLVED IN: DNA repair, response to UV-B, error-prone translesion synthesis; LOCATED IN: endomembrane system; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: DNA polymerase eta (InterPro:IPR017061), DNA-repair protein, UmuC-like (InterPro:IPR001126), DNA-repair protein, UmuC-like, N-terminal (InterPro:IPR017963); BEST Arabidopsis thaliana protein match is: DNA-directed DNA polymerases (TAIR:AT5G44750.1); Has 6919 Blast hits to 6912 proteins in 2220 species: Archae - 208; Bacteria - 5107; Metazoa - 464; Fungi - 358; Plants - 146; Viruses - 3; Other Eukaryotes - 633 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

18047733..18051896 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_123841.5 → NP_568638.2 Y-family DNA polymerase H [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_568638.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4ZYA7, A4ZYA8, O48586, Q8H1C5, Q8H2D5, Q9C5F5

UniProtKB/TrEMBL

A0A5S9YB25, A0A654G8I8, A0A7G2FEF3

Conserved Domains (2) summary

cd01702
Location:15 → 435

PolY_Pol_eta; DNA Polymerase eta

cd19318
Location:623 → 641

Rev1_UBM2; Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1
NM_001344572.1 → NP_001332153.1 Y-family DNA polymerase H [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178UIH9, A0A1P8BG35
NM_180799.2 → NP_851130.1 Y-family DNA polymerase H [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_851130.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178UIH9

Conserved Domains (1) summary

cl12025
Location:20 → 351

PolY; Y-family of DNA polymerases