AMSH3 associated molecule with the SH3 domain of STAM 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: AMSH3
Official Full Name: associated molecule with the SH3 domain of STAM 3
Primary source: TAIR:AT4G16144
Locus tag: AT4G16144
See related: Araport:AT4G16144
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: associated molecule with the SH3 domain of STAM 3
Summary: Encodes AMSH3, a deubiquitinating enzyme that hydrolyzes K48- and K63-linked ubiquitin chains in vitro. Required for intracellular trafficking and vacuole biogenesis.
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Genomic context

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See AMSH3 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 13

Sequence:: Chromosome: 4; NC_003075.7 (9137789..9141772, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AMSH3 activity is required for immunity.
Title: The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity.
SH3P2 is an ubiquitin-binding protein and functions together with ESCRT-I and the AMSH3.
Title: Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3.
Data indicate that ALIX (At1g15130) protein could function as an important regulator for AMSH-like ubiquitin thiolesterase 3 (AMSH3) function at the late endosomes.
Title: Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3.
These data indicate the importance of AMSH3 and the AMSH3-ESCRT-III interaction for deubiquitination and degradation of ubiquitinated membrane substrates in plants.
Title: The ESCRT-III-interacting deubiquitinating enzyme AMSH3 is essential for degradation of ubiquitinated membrane proteins in Arabidopsis thaliana.
Data show that AMSH1, an AMSH3-related DUB, interacts with the ESCRT-III subunit vacuolar protein sorting2.1 (VPS2.1) and that impairment of both AMSH1 and VPS2.1 causes early senescence and hypersensitivity to artificial carbon starvation in the dark.
Title: The deubiquitinating enzyme AMSH1 and the ESCRT-III subunit VPS2.1 are required for autophagic degradation in Arabidopsis.
AMSH3 interacts with ESCRT-III subunits VPS2.1 and VPS24.1. The interaction of ESCRT-III subunits with AMSH3 is mediated by the MIM1 domain and depends on the MIT domain of AMSH3.
Title: The Arabidopsis deubiquitinating enzyme AMSH3 interacts with ESCRT-III subunits and regulates their localization.
AMSH3 is a major deubiquitinating enzyme that is essential for proper vacuole biogenesis, trafficking from the Golgi to the vacuole and the vacuolar trafficking of endocytosed cargo.
Title: The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_148342.8

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables K63-linked deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables metal-dependent deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular transport	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in modification-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vacuole organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein K48-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein K63-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
NOT involved_in protein deneddylation	IDA Inferred from Direct Assay more info	PubMed
NOT involved_in protein desumoylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within vacuole organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: associated molecule with the SH3 domain of STAM 3

NP_680708.7

associated molecule with the SH3 domain of STAM 3 (AMSH3); CONTAINS InterPro DOMAIN/s: Mov34/MPN/PAD-1 (InterPro:IPR000555); BEST Arabidopsis thaliana protein match is: associated molecule with the SH3 domain of STAM 1 (TAIR:AT1G48790.1); Has 901 Blast hits to 707 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 373; Fungi - 228; Plants - 224; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink).