RPT5B 26S proteasome AAA-ATPase subunit RPT5B [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RPT5B
Official Full Name: 26S proteasome AAA-ATPase subunit RPT5B
Primary source: TAIR:AT1G09100
Locus tag: AT1G09100
See related: Araport:AT1G09100
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: 26S proteasome AAA-ATPase subunit RPT5B; F7G19.2; F7G19_2
Summary: Encodes RPT5b (Regulatory Particle 5b), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
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Genomic context

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See RPT5B in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 10

Sequence:: Chromosome: 1; NC_003070.9 (2936528..2939363, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing
Title: A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliana natural variation.
RPT5 subunits are essential for both male and female gametophyte development.
Title: The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development and show accession-dependent redundancy.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_100781.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within embryo sac development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within glucose mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pollen development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within proteasomal protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in extracellular region	HDA more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
part_of proteasome complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 26S proteasome AAA-ATPase subunit RPT5B

NP_172384.1

26S proteasome AAA-ATPase subunit RPT5B (RPT5B); FUNCTIONS IN: ATPase activity, calmodulin binding; INVOLVED IN: glucose mediated signaling pathway, proteasomal protein catabolic process, embryo sac development, pollen development; LOCATED IN: proteasome complex, plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: regulatory particle triple-A ATPase 5A (TAIR:AT3G05530.1); Has 33470 Blast hits to 31156 proteins in 3185 species: Archae - 1434; Bacteria - 12401; Metazoa - 4942; Fungi - 3663; Plants - 3251; Viruses - 53; Other Eukaryotes - 7726 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

2936528..2939363 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_100781.5 → NP_172384.1 26S proteasome AAA-ATPase subunit RPT5B [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_172384.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O04019, Q93V77

UniProtKB/TrEMBL

A0A178WHJ4, A0A5S9TDE0, A0A654EIU6

Conserved Domains (1) summary

COG1222
Location:28 → 416

RPT1; ATP-dependent 26S proteasome regulatory subunit [Posttranslational modification, protein turnover, chaperones]