RNEE/G RNAse E/G-like protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RNEE/G
Official Full Name: RNAse E/G-like protein
Primary source: TAIR:AT2G04270
Locus tag: AT2G04270
See related: Araport:AT2G04270
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: RNase E; RNAse E/G-like; RNE; T23O15.10; T23O15_10
Summary: Similar to E.coli endoribonuclease E. Functions as a ribonuclease, is located in the chloroplast, and is involved in chloroplast development. Loss of function mutants are white and arrest at the cotyledon stage. The phenotype is rescued by providing sucrose.
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Genomic context

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See RNEE/G in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 14

Sequence:: Chromosome: 2; NC_003071.7 (1474679..1480586)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis CSP41 proteins form multimeric complexes that bind and stabilize distinct plastid transcripts.
Title: Arabidopsis CSP41 proteins form multimeric complexes that bind and stabilize distinct plastid transcripts.
RNase E mutants have multiple defects in chloroplast RNA metabolism, but RNase E is not essential for survival.
Title: Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency.
Loss of At2g04270 function resulted in the arrest of chloroplast development, loss of autotrophic growth, and reduced plastid ribosomal, psbA and rbcL RNA levels.
Title: A 125 kDa RNase E/G-like protein is present in plastids and is essential for chloroplast development and autotrophic growth in Arabidopsis.
RNase E participates in the initial endonucleolytic cleavage of the polyadenylation-stimulated RNA degradation process in the chloroplast, perhaps in collaboration with the two other chloroplast endonucleases, RNase J and CSP41.
Title: The RNase E/G-type endoribonuclease of higher plants is located in the chloroplast and cleaves RNA similarly to the E. coli enzyme.

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General gene information

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Homology

Orthologs from OrthoDB

NM_001202581.2

Supported by ESTs:

NM_001335223.1

Supported by ESTs:

NM_001335224.1

Supported by ESTs:

NM_001335225.1

Supported by ESTs:

NM_001335226.1

Supported by ESTs:

NM_126460.4

Supported by ESTs:

NM_180656.2

Supported by ESTs:

NM_180657.2

Supported by ESTs:

NM_180658.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables starch binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chloroplast mRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chloroplast organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chloroplast rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: RNAse E/G-like protein

NP_001189510.1

RNAse E/G-like (RNEE/G); CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Carbohydrate-binding-like fold (InterPro:IPR013784), Ribonuclease E/G (InterPro:IPR004659), Glycoside hydrolase, carbohydrate-binding (InterPro:IPR002044), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Immunoglobulin-like fold (InterPro:IPR013783), RNA-binding protein AU-1/Ribonuclease E/G (InterPro:IPR019307).

NP_001318196.1

RNAse E/G-like (RNEE/G); FUNCTIONS IN: endoribonuclease activity; INVOLVED IN: chloroplast organization; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Nucleic acid-binding, OB-fold (InterPro:IPR012340), RNA-binding protein AU-1/Ribonuclease E/G (InterPro:IPR019307), Ribonuclease E/G (InterPro:IPR004659); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_850987.1

RNAse E/G-like (RNEE/G); FUNCTIONS IN: endoribonuclease activity; INVOLVED IN: chloroplast organization; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Carbohydrate-binding-like fold (InterPro:IPR013784), Ribonuclease E/G (InterPro:IPR004659), Glycoside hydrolase, carbohydrate-binding (InterPro:IPR002044), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Immunoglobulin-like fold (InterPro:IPR013783), RNA-binding protein AU-1/Ribonuclease E/G (InterPro:IPR019307); Has 10085 Blast hits to 8560 proteins in 1893 species: Archae - 5; Bacteria - 6357; Metazoa - 7; Fungi - 75; Plants - 95; Viruses - 0; Other Eukaryotes - 3546 (source: NCBI BLink).

NP_850988.1

RNAse E/G-like (RNEE/G); FUNCTIONS IN: endoribonuclease activity; INVOLVED IN: chloroplast organization; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), RNA-binding protein AU-1/Ribonuclease E/G (InterPro:IPR019307), Ribonuclease E/G (InterPro:IPR004659); Has 9824 Blast hits to 8321 proteins in 1821 species: Archae - 3; Bacteria - 6220; Metazoa - 3; Fungi - 1; Plants - 53; Viruses - 0; Other Eukaryotes - 3544 (source: NCBI BLink).

NP_850989.1

RNAse E/G-like (RNEE/G); FUNCTIONS IN: endoribonuclease activity; INVOLVED IN: chloroplast organization; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), RNA-binding protein AU-1/Ribonuclease E/G (InterPro:IPR019307), Ribonuclease E/G (InterPro:IPR004659); Has 9804 Blast hits to 8305 proteins in 1820 species: Archae - 3; Bacteria - 6220; Metazoa - 3; Fungi - 1; Plants - 53; Viruses - 0; Other Eukaryotes - 3524 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

1474679..1480586

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_180656.2 → NP_850987.1 RNAse E/G-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850987.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178VQ27, A0A5S9WX39, A0A654ERU6

Conserved Domains (3) summary

pfam00686
Location:77 → 172

CBM_20; Starch binding domain

pfam10150
Location:562 → 839

RNase_E_G; Ribonuclease E/G family

cl09927
Location:360 → 565

S1_like; Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. Both domains ...
NM_001335223.1 → NP_001318196.1 RNAse E/G-like protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4IV66
NM_001335224.1 → NP_001325371.1 RNAse E/G-like protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A654ERU6
NM_001335225.1 → NP_001325372.1 RNAse E/G-like protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A654ERU6, Q8GVF1
NM_001335226.1 → NP_001325370.1 RNAse E/G-like protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4IV66
NM_001202581.2 → NP_001189510.1 RNAse E/G-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189510.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4IV66, F4IV67, F4IV69, Q8GZQ4, Q8RWB3, Q9M498, Q9SI08

UniProtKB/TrEMBL

A0A654ERU6

Conserved Domains (4) summary

TIGR00757
Location:346 → 878

RNaseEG; ribonuclease, Rne/Rng family

pfam00686
Location:82 → 177

CBM_20; Starch binding domain

pfam10150
Location:567 → 844

RNase_E_G; Ribonuclease E/G family

cl09927
Location:365 → 570

S1_like; S1_like: Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. ...
NM_180657.2 → NP_850988.1 RNAse E/G-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850988.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A654ERU6

Conserved Domains (4) summary

TIGR00757
Location:216 → 748

RNaseEG; ribonuclease, Rne/Rng family

pfam10150
Location:437 → 714

RNase_E_G; Ribonuclease E/G family

cl09927
Location:235 → 440

S1_like; S1_like: Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. ...

cl15347
Location:11 → 47

CBM20; The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is ...
NM_126460.4 → NP_178508.2 RNAse E/G-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_178508.2

Status: REVIEWED

UniProtKB/Swiss-Prot

F4IV66

Conserved Domains (3) summary

TIGR00757
Location:58 → 590

RNaseEG; ribonuclease, Rne/Rng family

pfam10150
Location:279 → 556

RNase_E_G; Ribonuclease E/G family

cl09927
Location:251 → 282

S1_like; S1_like: Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. ...
NM_180658.2 → NP_850989.1 RNAse E/G-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850989.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4IV66

Conserved Domains (3) summary

TIGR00757
Location:58 → 590

RNaseEG; ribonuclease, Rne/Rng family

pfam10150
Location:279 → 556

RNase_E_G; Ribonuclease E/G family

cl09927
Location:77 → 282

S1_like; S1_like: Ribosomal protein S1-like RNA-binding domain. Found in a wide variety of RNA-associated proteins. Originally identified in S1 ribosomal protein. This superfamily also contains the Cold Shock Domain (CSD), which is a homolog of the S1 domain. ...