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    RCA rubisco activase [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 818558, updated on 18-Sep-2024

    Summary

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    Official Symbol
    RCA
    Official Full Name
    rubisco activase
    Primary source
    TAIR:AT2G39730
    Locus tag
    AT2G39730
    See related
    Araport:AT2G39730
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    rubisco activase; RUBISCO ACTIVASE; T5I7.18
    Summary
    Rubisco activase, a nuclear-encoded chloroplast protein that consists of two isoforms arising from alternative splicing in most plants. Required for the light activation of rubisco. Involved in jasmonate-induced leaf senescence.
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    Genomic context

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    See RCA in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    7
    Sequence:
    Chromosome: 2; NC_003071.7 (16570746..16573692, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Eukaryotic aspartyl protease family protein Neighboring gene RING-H2 finger C2A Neighboring gene LYR family of Fe/S cluster biogenesis protein Neighboring gene Nucleotidyltransferase family protein Neighboring gene S-adenosyl-L-methionine-dependent methyltransferases superfamily protein Neighboring gene BTB/POZ/MATH-domains containing protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Arabidopsis plants expressing only the redox-regulated Rca-α isoform have constrained photosynthesis and plant growth. Kim SY, et al. Plant J, 2020 Sep. PMID 32593186
    2. Identification of an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) minimal promoter regulated by light and the circadian clock. Liu Z, et al. Plant Physiol, 1996 Sep. PMID 8819320, Free PMC Article
    3. Mechanism of light regulation of Rubisco: a specific role for the larger Rubisco activase isoform involving reductive activation by thioredoxin-f. Zhang N, et al. Proc Natl Acad Sci U S A, 1999 Aug 3. PMID 10430961, Free PMC Article
    4. Increased sensitivity of oxidized large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase to ADP inhibition is due to an interaction between its carboxyl extension and nucleotide-binding pocket. Wang D, et al. J Biol Chem, 2006 Sep 1. PMID 16822862
    5. Effect of activase level and isoform on the thermotolerance of photosynthesis in Arabidopsis. Salvucci ME, et al. J Exp Bot, 2006. PMID 16997900

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Arabidopsis plants expressing only the redox-regulated Rca-alpha isoform have constrained photosynthesis and plant growth.
      Title: Arabidopsis plants expressing only the redox-regulated Rca-α isoform have constrained photosynthesis and plant growth.
    2. Rubisco activase requires residues in the large subunit N terminus to remodel inhibited plant Rubisco.
      Title: Rubisco activase requires residues in the large subunit N terminus to remodel inhibited plant Rubisco.
    3. Improving drought-, salinity-, and heat-tolerance in transgenic plants by co-overexpressing Arabidopsis vacuolar pyrophosphatase gene AVP1 and Larrea Rubisco activase gene RCA.
      Title: Improving drought-, salinity-, and heat-tolerance in transgenic plants by co-overexpressing Arabidopsis vacuolar pyrophosphatase gene AVP1 and Larrea Rubisco activase gene RCA.
    4. Study shows a regulatory role of Arabidopsis Rca phosphorylation at the Thr78 site. Conservative substitution of serine for threonine-78 (T78S) results in impaired functionality of Rca in vivo. Plants expressing the T78S mutation had reduced photosynthesis and quantum efficiency of photosystem II and reduced growth relative to control plants expressing wild-type Rca-beta under all conditions tested.
      Title: In vivo evidence for a regulatory role of phosphorylation of Arabidopsis Rubisco activase at the Thr78 site.
    5. Thecrystal structure of Rubisco activase from Arabidopsis thaliana is presented at 2.9 A resolution.
      Title: Structure of Arabidopsis thaliana Rubisco activase.
    6. Phosphorylation thus appears to be a potential mechanism associated with RCA dark inactivation, when Rubisco-catalysed carboxylation is arrested.
      Title: Phosphorylation pattern of Rubisco activase in Arabidopsis leaves.
    7. manipulation of Rca regulation might provide a strategy for enhancing photosynthetic performance in certain variable light environments.
      Title: The regulatory properties of Rubisco activase differ among species and affect photosynthetic induction during light transitions.
    8. These results confirmed the exceptional thermal lability of activase at physiological ratios of activase to Rubisco.
      Title: The activity of Rubisco's molecular chaperone, Rubisco activase, in leaf extracts.
    9. Data suggested that the COI1-dependent JA repression of RCA played an important role in JA-induced leaf senescence.
      Title: New perspective of jasmonate function in leaf senescence.
    10. The COI1-dependent JA repression of RCA correlated with JA-induced leaf senescence.
      Title: The role of Arabidopsis Rubisco activase in jasmonate-induced leaf senescence.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_129531.3
    NM_179989.3
    NM_179990.1

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ADP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme regulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within leaf senescence IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to jasmonic acid IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to light stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus HDA PubMed 
    located_in apoplast HDA PubMed 
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast NAS
    Non-traceable Author Statement
    more info
    		  
    located_in chloroplast envelope HDA PubMed 
    located_in chloroplast stroma HDA PubMed 
    located_in chloroplast thylakoid membrane HDA PubMed 
    located_in nucleus HDA PubMed 
    located_in plant-type cell wall HDA PubMed 
    located_in stromule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in thylakoid HDA PubMed 

    General protein information

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    Preferred Names
    rubisco activase
    NP_565913.1
    • rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 11 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2406 Blast hits to 2358 proteins in 526 species: Archae - 328; Bacteria - 489; Metazoa - 254; Fungi - 429; Plants - 406; Viruses - 0; Other Eukaryotes - 500 (source: NCBI BLink).
    NP_850320.1
    • rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2331 Blast hits to 2283 proteins in 512 species: Archae - 328; Bacteria - 459; Metazoa - 248; Fungi - 427; Plants - 404; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).
    NP_850321.1
    • rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2322 Blast hits to 2274 proteins in 510 species: Archae - 328; Bacteria - 451; Metazoa - 248; Fungi - 425; Plants - 405; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      16570746..16573692 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_129531.3 → NP_565913.1  rubisco activase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_565913.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P10896, Q39197, Q39198, Q8H172, Q940T8, Q941B7
      UniProtKB/TrEMBL
      A0A5S9X620, A0A654F1P8
      Conserved Domains (1) summary
      PLN00020
      Location:8 → 422
      PLN00020; ribulose bisphosphate carboxylase/oxygenase activase -RuBisCO activase (RCA); Provisional

    2. NM_179989.3 → NP_850320.1  rubisco activase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850320.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178VW63
      Conserved Domains (1) summary
      PLN00020
      Location:8 → 422
      PLN00020; ribulose bisphosphate carboxylase/oxygenase activase -RuBisCO activase (RCA); Provisional

    3. NM_179990.1 → NP_850321.1  rubisco activase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850321.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178VUM0, F4IVZ7
      Conserved Domains (1) summary
      PLN00020
      Location:8 → 422
      PLN00020; ribulose bisphosphate carboxylase/oxygenase activase -RuBisCO activase (RCA); Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P10896.2

    Gene LinkOut

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