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    RALF1 rapid alkalinization factor 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 839389, updated on 18-Sep-2024

    Summary

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    Official Symbol
    RALF1
    Official Full Name
    rapid alkalinization factor 1
    Primary source
    TAIR:AT1G02900
    Locus tag
    AT1G02900
    See related
    Araport:AT1G02900
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATRALF1; F22D16.10; F22D16_10; RALF-LIKE 1; RALFL1; rapid alkalinization factor 1; RAPID ALKALINIZATION FACTOR 1
    Summary
    Member of a diversely expressed predicted peptide family showing sequence similarity to tobacco Rapid Alkalinization Factor (RALF), and is believed to play an essential role in the physiology of Arabidopsis. Consists of a single exon and is characterized by a conserved C-terminal motif and N-terminal signal peptide. Mediates Ca2+-dependent signaling.
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    Genomic context

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    See RALF1 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    1
    Sequence:
    Chromosome: 1; NC_003070.9 (653604..654457, complement)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene thiamine pyrophosphokinase1 Neighboring gene AAA-type ATPase family protein Neighboring gene tetratricopeptide repeat (TPR)-containing protein Neighboring gene glutathione S-transferase 7

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis. Li L, et al. Proc Natl Acad Sci U S A, 2022 Aug 2. PMID 35878023, Free PMC Article
    2. A cytoplasmic Ca2+ functional assay for identifying and purifying endogenous cell signaling peptides in Arabidopsis seedlings: identification of AtRALF1 peptide. Haruta M, et al. Biochemistry, 2008 Jun 17. PMID 18494498
    3. A conserved dibasic site is essential for correct processing of the peptide hormone AtRALF1 in Arabidopsis thaliana. Matos JL, et al. FEBS Lett, 2008 Oct 15. PMID 18775699
    4. Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation. Bergonci T, et al. J Exp Bot, 2014 May. PMID 24620000, Free PMC Article
    5. Antagonistic relationship between AtRALF1 and brassinosteroid regulates cell expansion-related genes. Bergonci T, et al. Plant Signal Behav, 2014. PMID 25482784, Free PMC Article

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
      Title: Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
    2. RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
      Title: RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
    3. The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth.
      Title: The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth.
    4. inter-relationships with the heterotrimeric Gbeta subunit AGB1 and FERONIA in salinity response
      Title: Inter-relationships between the heterotrimeric Gβ subunit AGB1, the receptor-like kinase FERONIA, and RALF1 in salinity response.
    5. Restoration of guard cell rapid alkalinization factor 1 (RALF1) responses by dereceptor-like kinase FERONIA (FER) is dose-dependent.
      Title: A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.
    6. We report here that, upon RALF1 binding, FER first promotes ErbB3-binding protein 1 (EBP1) mRNA translation and then interacts with and phosphorylates the EBP1 protein, leading to EBP1 accumulation in the nucleus.
      Title: EBP1 nuclear accumulation negatively feeds back on FERONIA-mediated RALF1 signaling.
    7. RALF1 role in stomatal movement regulation.
      Title: The G Protein β-Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement.
    8. a new component of the AtRALF1 response pathway is a calmodulin-like protein that binds AtRALF1, is essential for root growth inhibition, and has no role in AtRALF1 alkalinization.
      Title: Arabidopsis thaliana rapid alkalinization factor 1-mediated root growth inhibition is dependent on calmodulin-like protein 38.
    9. RALF1 triggers the phosphorylation of both FER and RIPK in a mutually dependent manner
      Title: Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis.
    10. suppression of RALF1 gene expression, similar to disruption of the FER gene, rendered plants hypersensitive to ABA. These results formulated a mechanism for ABA activation of FER and for cross-talk between ABA and peptide hormone RALF in the control of plant growth and responses to stress signals
      Title: FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_100171.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables hormone activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within calcium-mediated signaling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cell-cell signaling ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    involved_in negative regulation of cell growth IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in root development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in apoplast ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    located_in extracellular region ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    rapid alkalinization factor 1
    NP_171789.1
    • rapid alkalinization factor 1 (RALF1); CONTAINS InterPro DOMAIN/s: Rapid ALkalinization Factor (InterPro:IPR008801); BEST Arabidopsis thaliana protein match is: ralf-like 33 (TAIR:AT4G15800.1); Has 267 Blast hits to 267 proteins in 20 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 2; Plants - 265; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      653604..654457 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_100171.3 → NP_171789.1  rapid alkalinization factor 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_171789.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9SRY3
      UniProtKB/TrEMBL
      A0A178WER1, A0A5S9SBJ1
      Conserved Domains (1) summary
      cl23823
      Location:45 → 119
      RALF; Rapid ALkalinization Factor (RALF)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SRY3.1

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