GSH2 glutathione synthetase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Official Symbol: GSH2
Official Full Name: glutathione synthetase 2
Primary source: TAIR:AT5G27380
Locus tag: AT5G27380
See related: Araport:AT5G27380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtGSH2; F21A20.90; F21A20_90; GLUTATHIONE SYNTHETASE; glutathione synthetase 2; GSHB
Summary: Encodes a protein with similarity to glutathione synthetases, which catalyzes one of the early steps in glutathione biosynthesis. Two transcripts have been detected; the longer transcript is less abundant and the protein is localized to the chloroplast. The smaller transcript, in which the transit peptide is truncated, is localized to the cytosol.
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Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Redox-sensitive GFP revealed that gsh2 seedlings maintained redox poise in the cytoplasm but were more sensitive to oxidative challenge.
Title: A perturbation in glutathione biosynthesis disrupts endoplasmic reticulum morphology and secretory membrane traffic in Arabidopsis thaliana.
Mutation in glutathione synthetase gene impaired cadmium-induced sulfate assimilation.
Title: Feedback inhibition by thiols outranks glutathione depletion: a luciferase-based screen reveals glutathione-deficient γ-ECS and glutathione synthetase mutants impaired in cadmium-induced sulfate assimilation.
Comparison of the pH profiles and the solvent deuterium isotope effects of A. thaliana glutathione synthetase and the Arg-132 and Arg-454 mutants suggest distinct mechanistic roles for these residues
Title: Reaction mechanism of glutathione synthetase from Arabidopsis thaliana: site-directed mutagenesis of active site residues.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

NM_122620.4

Function	Evidence Code	Pubs
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables glutathione binding	ISS Inferred from Sequence or Structural Similarity more info
enables glutathione synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutathione synthase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within glutathione biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutathione biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glutathione biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to jasmonic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to stress	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info	PubMed
located_in plastid	HDA more info	PubMed

NP_568495.2

glutathione synthetase 2 (GSH2); FUNCTIONS IN: glutathione synthase activity; INVOLVED IN: response to jasmonic acid stimulus, N-terminal protein myristoylation, glutathione biosynthetic process; LOCATED IN: cytosol, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: PreATP-grasp-like fold (InterPro:IPR016185), ATP-grasp fold, subdomain 2 (InterPro:IPR013816), Glutathione synthase, eukaryotic (InterPro:IPR005615), Glutathione synthase, substrate-binding, eukaryotic (InterPro:IPR004887), Glutathione synthase, alpha-helical, eukaryotic (InterPro:IPR014042); Has 644 Blast hits to 607 proteins in 239 species: Archae - 0; Bacteria - 51; Metazoa - 212; Fungi - 156; Plants - 112; Viruses - 0; Other Eukaryotes - 113 (source: NCBI BLink).