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    TCH2 EF hand calcium-binding protein family [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 833755, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TCH2
    Official Full Name
    EF hand calcium-binding protein family
    Primary source
    TAIR:AT5G37770
    Locus tag
    AT5G37770
    See related
    Araport:AT5G37770
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtCML24; CALMODULIN-LIKE 24; CML24; K22F20.10; K22F20_10; TOUCH 2
    Summary
    Encodes a protein with 40% similarity to calmodulin. Binds Ca(2+) and, as a consequence, undergoes conformational changes. CML24 expression occurs in all major organs, and transcript levels are increased from 2- to 15-fold in plants subjected to touch, darkness, heat, cold, hydrogen peroxide, abscisic acid (ABA), and indole-3-acetic acid. However, CML24 protein accumulation changes were not detectable. The putative CML24 regulatory region confers reporter expression at sites of predicted mechanical stress; in regions undergoing growth; in vascular tissues and various floral organs; and in stomata, trichomes, and hydathodes. CML24-underexpressing transgenics are resistant to ABA inhibition of germination and seedling growth, are defective in long-day induction of flowering, and have enhanced tolerance to CoCl(2), molybdic acid, ZnSO(4), and MgCl(2). Also regulates nitric oxide levels.
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    Genomic context

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    See TCH2 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    2
    Sequence:
    Chromosome: 5; NC_003076.8 (14997514..14999619, complement)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene Calcium-dependent lipid-binding (CaLB domain) family protein Neighboring gene Chaperone DnaJ-domain superfamily protein Neighboring gene Chaperone DnaJ-domain superfamily protein Neighboring gene calmodulin 1 Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Arabidopsis thaliana calmodulin-like protein CML24 regulates pollen tube growth by modulating the actin cytoskeleton and controlling the cytosolic Ca(2+) concentration. Yang X, et al. Plant Mol Biol, 2014 Oct. PMID 25139229
    2. Innate immunity signaling: cytosolic Ca2+ elevation is linked to downstream nitric oxide generation through the action of calmodulin or a calmodulin-like protein. Ma W, et al. Plant Physiol, 2008 Oct. PMID 18689446, Free PMC Article
    3. CML24, regulated in expression by diverse stimuli, encodes a potential Ca2+ sensor that functions in responses to abscisic acid, daylength, and ion stress. Delk NA, et al. Plant Physiol, 2005 Sep. PMID 16113225, Free PMC Article
    4. Comparative modeling of the three-dimensional structure of the calmodulin-related TCH2 protein from Arabidopsis. Khan AR, et al. Proteins, 1997 Jan. PMID 9037719
    5. Regulated expression of the calmodulin-related TCH genes in cultured Arabidopsis cells: induction by calcium and heat shock. Braam J. Proc Natl Acad Sci U S A, 1992 Apr 15. PMID 1373491, Free PMC Article

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These results support the conclusion that CML24 affects autophagy progression through interactions with ATG4.
      Title: Calmodulin-related CML24 interacts with ATG4b and affects autophagy progression in Arabidopsis.
    2. AtCML24 acts as a regulator of pollen germination and pollen tube extension.
      Title: Arabidopsis thaliana calmodulin-like protein CML24 regulates pollen tube growth by modulating the actin cytoskeleton and controlling the cytosolic Ca(2+) concentration.
    3. The calmodulin-like protein CML24 is activated by Ca2+ influx into the cytosol, which induces nitric oxide generation, leading to an innate immune response.
      Title: Innate immunity signaling: cytosolic Ca2+ elevation is linked to downstream nitric oxide generation through the action of calmodulin or a calmodulin-like protein.
    4. CML24, a gene expressed in diverse organs and responsive to diverse stimuli, encodes a potential Ca(2+) sensor that may function to enable responses to ABA, daylength, and presence of various salts.[CML24]
      Title: CML24, regulated in expression by diverse stimuli, encodes a potential Ca2+ sensor that functions in responses to abscisic acid, daylength, and ion stress.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001344218.1
    NM_123136.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables calcium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calcium ion binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within innate immune response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within long-day photoperiodism, flowering IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of flower development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of nitric oxide metabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    NOT involved_in response to 1-aminocyclopropane-1-carboxylic acid IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to abscisic acid IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to abscisic acid IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to absence of light IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to auxin IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to calcium ion IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    NOT involved_in response to gibberellin IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to heat IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to hydrogen peroxide IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to mechanical stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to mechanical stimulus TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to metal ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    colocalizes_with actin cytoskeleton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    EF hand calcium-binding protein family
    NP_001318691.1
    • TOUCH 2 (TCH2); FUNCTIONS IN: calcium ion binding; INVOLVED IN: in 13 processes; LOCATED IN: plasma membrane, actin cytoskeleton; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: EF-Hand 1, calcium-binding site (InterPro:IPR018247), EF-HAND 2 (InterPro:IPR018249), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand (InterPro:IPR018248); BEST Arabidopsis thaliana protein match is: calmodulin like 23 (TAIR:AT1G66400.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      14997514..14999619 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001344218.1 → NP_001318691.1  EF hand calcium-binding protein family [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O22592, P25070, Q1ECM5
      UniProtKB/TrEMBL
      A0A178UBB8, A0A5S9Y8W0
      Conserved Domains (1) summary
      PTZ00184
      Location:19 → 155
      PTZ00184; calmodulin; Provisional

    2. NM_123136.3 → NP_198593.1  EF hand calcium-binding protein family [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_198593.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O22592, P25070, Q1ECM5
      UniProtKB/TrEMBL
      A0A178UBB8, A0A5S9Y8W0
      Conserved Domains (1) summary
      PTZ00184
      Location:19 → 155
      PTZ00184; calmodulin; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25070.2

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