E(bx) Enhancer of bithorax [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: E(bx)provided by FlyBase
Official Full Name: Enhancer of bithoraxprovided by FlyBase
Primary source: FLYBASE:FBgn0000541
Locus tag: Dmel_CG32346
See related: AllianceGenome:FB:FBgn0000541
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG10894; CG17135; CG32346; CG32478; CG7022; Dmel\CG32346; dNURF; dNURF301; e(bx); e(Bx); E(Bx); E(BX); Ebx; En-bx; l(3)122; l(3)ry122; Nurf; NURF; NURF 215; Nurf 301; Nurf-215; NURF-215; NURF-301; NURF215; nurf301; Nurf301; NuRF301; NURF301; p215; p301
Summary: Enables histone binding activity; nuclear receptor binding activity; and transcription coactivator activity. Involved in several processes, including negative regulation of receptor signaling pathway via JAK-STAT; nucleosome organization; and regulation of DNA-templated transcription. Located in nucleus. Part of NURF complex. Is expressed in several structures, including adult head; male germline cell; and somatic cell of testis. Human ortholog(s) of this gene implicated in Kaposi's sarcoma; alcohol dependence; and neurodevelopmental disorder with dysmorphic facies and distal limb anomalies. Orthologous to human BPTF (bromodomain PHD finger transcription factor). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 61B2-61B3; 3-1 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (233926..246912, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (233926..246912, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.
Title: NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.
The data show that, besides anticipated roles at enhancers, NURF interacts physically and functionally with the TRF2/DREF basal transcription factor to organize nucleosomes downstream of active promoters.
Title: Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators.
required for stem cell maintenance in the Drosophila testis
Title: Epigenetic regulation of stem cell maintenance in the Drosophila testis via the nucleosome-remodeling factor NURF.
Study demonstrates that three NURF301 isoforms are expressed and that these encode functionally distinct NURF chromatin remodeling complexes.
Title: Alternative splicing of NURF301 generates distinct NURF chromatin remodeling complexes with altered modified histone binding specificities.
Chromatin immunoprecipitation localizes NURF to Ken-binding sites in hemocytes, suggesting that Ken recruits NURF.
Title: The nucleosome remodeling factor (NURF) regulates genes involved in Drosophila innate immunity.
sliding model in which the position-specific tethering of NURF forces a translocating ISWI ATPase to pump a DNA distortion over the histone octamer, thereby changing the translational position of the nucleosome.
Title: Spatial contacts and nucleosome step movements induced by the NURF chromatin remodeling complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables lysine-acetylated histone binding	IDA Inferred from Direct Assay more info	PubMed
enables methylated histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables methylated histone binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hemopoiesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of receptor signaling pathway via JAK-STAT	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleosome organization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatid differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NURF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of NURF complex	IDA Inferred from Direct Assay more info	PubMed
part_of NURF complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: enhancer of bithorax

Names: CG32346-PA; CG32346-PB; CG32346-PC; CG32346-PE; CG32346-PF; CG32346-PG; CG32346-PH; CG32346-PI; CG32346-PJ; E(bx)-PA; E(bx)-PB; E(bx)-PC; E(bx)-PE; E(bx)-PF; E(bx)-PG; E(bx)-PH; E(bx)-PI; E(bx)-PJ; nucleosome remodeling factor; nucleosome remodeling factor - 215kD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

233926..246912 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001274261.1 → NP_001261190.1 enhancer of bithorax, isoform J [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q4V464, Q7KVD8, Q95VB8, Q960Y3, Q9W0T1, Q9W0T2

UniProtKB/TrEMBL

E1JHV6

Conserved Domains (8) summary

cd05509
Location:2561 → 2660

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2331 → 2546

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2497 → 2543

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2441 → 2487

PHD_SF; PHD finger superfamily
NM_001274260.1 → NP_001261189.1 enhancer of bithorax, isoform I [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

M9PE12

Conserved Domains (8) summary

cd05509
Location:2560 → 2659

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2330 → 2545

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2496 → 2542

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2440 → 2486

PHD_SF; PHD finger superfamily
NM_001274258.1 → NP_001261187.1 enhancer of bithorax, isoform G [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

M9PBG5

Conserved Domains (4) summary

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...
NM_001169833.2 → NP_001163304.1 enhancer of bithorax, isoform E [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q4V464, Q7KVD8, Q95VB8, Q960Y3, Q9W0T1, Q9W0T2

UniProtKB/TrEMBL

E1JHV6

Conserved Domains (8) summary

cd05509
Location:2561 → 2660

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2331 → 2546

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2497 → 2543

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2441 → 2487

PHD_SF; PHD finger superfamily
NM_167817.3 → NP_728505.1 enhancer of bithorax, isoform B [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9W0T1

Related

FBpp0072422

Conserved Domains (8) summary

cd05509
Location:2541 → 2640

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2311 → 2526

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2477 → 2523

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2421 → 2467

PHD_SF; PHD finger superfamily
NM_167819.3 → NP_728507.1 enhancer of bithorax, isoform A [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q4V464, Q7KVD8, Q95VB8, Q960Y3, Q9W0T1, Q9W0T2

UniProtKB/TrEMBL

E1JHV6

Related

FBpp0072421

Conserved Domains (8) summary

cd05509
Location:2561 → 2660

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2331 → 2546

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2497 → 2543

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2441 → 2487

PHD_SF; PHD finger superfamily
NM_001274259.1 → NP_001261188.1 enhancer of bithorax, isoform H [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

M9PDH1

Conserved Domains (8) summary

cd05509
Location:2653 → 2752

Bromo_gcn5_like; Bromodomain; Gcn5_like subfamily. Gcn5p is a histone acetyltransferase (HAT) which mediates acetylation of histones at lysine residues; such acetylation is generally correlated with the activation of transcription. Bromodomains are 110 amino acid long ...

COG5034
Location:2423 → 2638

TNG2; Chromatin remodeling protein, contains PhD zinc finger [Chromatin structure and dynamics]

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

cd15560
Location:2589 → 2635

PHD2_3_BPTF; PHD finger 2 and 3 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...

cl22851
Location:2533 → 2579

PHD_SF; PHD finger superfamily
NM_001169834.2 → NP_001163305.1 enhancer of bithorax, isoform F [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

E1JHV7

Conserved Domains (4) summary

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...
NM_206224.3 → NP_995946.1 enhancer of bithorax, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9W0T1

Conserved Domains (4) summary

cd15559
Location:341 → 383

PHD1_BPTF; PHD finger 1 found in bromodomain and PHD finger-containing transcription factor (BPTF)

pfam02791
Location:190 → 245

DDT; DDT domain

pfam15612
Location:288 → 337

WHIM1; WSTF, HB1, Itc1p, MBD9 motif 1

cl02608
Location:361 → 438

BAH; BAH, or Bromo Adjacent Homology domain (also called ELM1 and BAM for Bromo Adjacent Motif). BAH domains have first been described as domains found in the polybromo protein and Yeast Rsc1/Rsc2 (Remodeling of the Structure of Chromatin). They also occur in ...