let-99 Protein let-99 [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: let-99
Official Full Name: Protein let-99
Primary source: WormBase:WBGene00002368
Locus tag: CELE_K08E7.3
See related: AllianceGenome:WB:WBGene00002368
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Involved in establishment of mitotic spindle orientation. Located in cell cortex. Is expressed in embryonic cell. [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See let-99 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 5

Sequence:: Chromosome: IV; NC_003282.8 (12567842..12570622)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells.
Title: LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.
LET-99 acts in the Mes-1/Src-1 pathway for mitotic spindle positioning.
Title: Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA.
Results indicate that PAR-5 protein acts in a mechanism with PAR-1 protein to regulate LET-99 protein cortical localization.
Title: The 14-3-3 protein PAR-5 regulates the asymmetric localization of the LET-99 spindle positioning protein.
The DEP domain protein LET-99 acts upstream of the heterotrimeric G protein alpha subunit GPA-11 to control activation of the stress kinase JNK-1.
Title: DEPDC1/LET-99 participates in an evolutionarily conserved pathway for anti-tubulin drug-induced apoptosis.
LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos
Title: LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos.
PAR-3 and PAR-1 have graded cortical distributions with the highest levels at the anterior and posterior poles, respectively, and the lowest levels of these proteins correlate with high LET-99 accumulation
Title: PAR-3 and PAR-1 inhibit LET-99 localization to generate a cortical band important for spindle positioning in Caenorhabditis elegans embryos.
Data show that the asymmetry of GPR-1/2 (which act with GOA-1 and GPA-16) localization depends on PAR-3 and its downstream intermediate LET-99, as well as on the MES-1/SRC-1 signaling pathway.
Title: LET-99 opposes Galpha/GPR signaling to generate asymmetry for spindle positioning in response to PAR and MES-1/SRC-1 signaling.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

K08E7.3 (e-PCR)
- UniSTS:311479

K08E7.2 (e-PCR)
- UniSTS:321005

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Protein let-99

NP_502407.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

12567842..12570622

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_070006.4 → NP_502407.1 Protein let-99 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502407.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q21341

Conserved Domains (1) summary

smart00049
Location:23 → 107

DEP; Domain found in Dishevelled, Egl-10, and Pleckstrin