ACA8 autoinhibited Ca2+ -ATPase [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: ACA8
Official Full Name: autoinhibited Ca2+ -ATPase
Primary source: TAIR:AT5G57110
Locus tag: AT5G57110
See related: Araport:AT5G57110
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ''autoinhibited Ca2+ -ATPase; ''AUTOINHIBITED CA2+ -ATPASE; 'autoinhibited Ca2+ -ATPase; AT-ACA8; autoinhibited Ca2+ -ATPase; AUTOINHIBITED CA2+ -ATPASE; isoform 8; ISOFORM 8; isoform 8'; isoform 8''; ISOFORM 8''; MUL3.5; MUL3_5
Summary: Arabidopsis-autoinhibited Ca2+ -ATPase, isoform 8, contains all of the characteristic motifs of Ca2+ -transporting P-type Ca2+ -ATPases and is localized to the plasma membrane.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See ACA8 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 36

Sequence:: Chromosome: 5; NC_003076.8 (23109438..23117686, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conserved N-terminal Regulation of the ACA8 Calcium Pump with Two Calmodulin Binding Sites.
Title: Conserved N-terminal Regulation of the ACA8 Calcium Pump with Two Calmodulin Binding Sites.
This study indicates that ACA10/8 and BON1 physically interact on plasma membrane and function in the generation of cytosol calcium signatures that are critical for stomatal movement and impact plant immunity.
Title: Calcium Pumps and Interacting BON1 Protein Modulate Calcium Signature, Stomatal Closure, and Plant Immunity.
This study identifies ACA8 as a prominent in vivo regulator of cellular Ca2+ dynamics and reveal the existence of a Ca2+-dependent CBL-CIPK-mediated regulatory feedback mechanism, which crucially functions in the termination of Ca2+ signals.
Title: Ca2+-dependent phosphoregulation of the plasma membrane Ca2+-ATPase ACA8 modulates stimulus-induced calcium signatures.
CML36 interacts directly with the regulative N terminus of the Arabidopsis plasma membrane Ca(2+)-ATPase isoform 8 (ACA8) and this interaction stimulates ACA8 activity
Title: Arabidopsis calmodulin-like protein CML36 is a calcium (Ca(2+)) sensor that interacts with the plasma membrane Ca(2+)-ATPase isoform ACA8 and stimulates its activity.
ACA8-mediated Ca(2+) signalling contributes to modulate early seedling development and coordinates root development with nutrient availability.
Title: The plasma membrane-localised Ca(2+)-ATPase ACA8 plays a role in sucrose signalling involved in early seedling development in Arabidopsis.
ACA8 and ACA10 contribute to plant immunity and have a role in plant development.
Title: Plasma membrane calcium ATPases are important components of receptor-mediated signaling in plant immune responses and development.
Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana.
Title: Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana.
regulatory domain works independently of its terminal localization
Title: Plant and animal type 2B Ca2+-ATPases: evidence for a common auto-inhibitory mechanism.
complex between calmodulin and the regulatory domain of the plasma-membrane Ca(2+)-ATPase ACA8 from Arabidopsis has been crystallized. Crystals belonged to space group C2, with unit-cell parameters a = 176.8, b = 70.0, c = 69.8 A, beta = 113.2 degrees.
Title: Expression, purification, crystallization and preliminary X-ray analysis of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8.
The small cytoplasmic loop of a type 2B Ca(2+)-ATPase plays a role in the attainment of the auto-inhibited state.
Title: Single point mutations in the small cytoplasmic loop of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana, generate partially deregulated pumps.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_001345242.1

Supported by ESTs:

NM_125093.6

Supported by ESTs:

NM_180869.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables P-type calcium transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables P-type calcium transporter activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables calmodulin binding	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to nematode	HEP more info	PubMed

Component	Evidence Code	Pubs
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasmodesma	HDA more info	PubMed
located_in plastid	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: autoinhibited Ca2+ -ATPase

NP_200521.3

''autoinhibited Ca2+ -ATPase, isoform 8'' (ACA8); FUNCTIONS IN: protein self-association, calcium-transporting ATPase activity, calmodulin binding; INVOLVED IN: response to nematode; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, P-type, ATPase-associated domain (InterPro:IPR008250), ATPase, P-type, calcium-transporting, PMCA-type (InterPro:IPR006408), Haloacid dehalogenase-like hydrolase (InterPro:IPR005834), ATPase, P-type cation-transporter, N-terminal (InterPro:IPR004014), ATPase, P-type, H+ transporting proton pump (InterPro:IPR000695), ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (InterPro:IPR001757), ATPase, P-type cation-transporter, C-terminal (InterPro:IPR006068), ATPase, P-type phosphorylation site (InterPro:IPR018303); BEST Arabidopsis thaliana protein match is: autoinhibited Ca(2+)-ATPase 10 (TAIR:AT4G29900.1); Has 45517 Blast hits to 34552 proteins in 3221 species: Archae - 872; Bacteria - 31142; Metazoa - 4022; Fungi - 2709; Plants - 2112; Viruses - 3; Other Eukaryotes - 4657 (source: NCBI BLink).

NP_851200.1

''autoinhibited Ca2+ -ATPase, isoform 8'' (ACA8); FUNCTIONS IN: protein self-association, calcium-transporting ATPase activity, calmodulin binding; INVOLVED IN: response to nematode; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, P-type, ATPase-associated domain (InterPro:IPR008250), ATPase, P-type, calcium-transporting, PMCA-type (InterPro:IPR006408), ATPase, P-type, H+ transporting proton pump (InterPro:IPR000695), ATPase, P-type cation-transporter, N-terminal (InterPro:IPR004014), Haloacid dehalogenase-like hydrolase (InterPro:IPR005834), ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (InterPro:IPR001757), ATPase, P-type phosphorylation site (InterPro:IPR018303), ATPase, P-type cation-transporter, C-terminal (InterPro:IPR006068); BEST Arabidopsis thaliana protein match is: autoinhibited Ca(2+)-ATPase 10 (TAIR:AT4G29900.1); Has 45378 Blast hits to 34457 proteins in 3207 species: Archae - 868; Bacteria - 31014; Metazoa - 4020; Fungi - 2704; Plants - 2112; Viruses - 3; Other Eukaryotes - 4657 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

23109438..23117686 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_125093.6 → NP_200521.3 autoinhibited Ca2+ -ATPase, isoform 8 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_200521.3

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LF79, Q9LU75

UniProtKB/TrEMBL

A0A178UCR0, A0A384L942, B9DH83, Q0WV19

Conserved Domains (2) summary

TIGR01517
Location:133 → 1047

ATPase-IIB_Ca; plasma-membrane calcium-translocating P-type ATPase

pfam12515
Location:30 → 71

CaATP_NAI; Ca2+-ATPase N terminal autoinhibitory domain
NM_180869.3 → NP_851200.1 autoinhibited Ca2+ -ATPase, isoform 8 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_851200.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LF79, Q9LU75

UniProtKB/TrEMBL

A0A178UCR0, A0A384L942, B9DH83, Q0WV19

Conserved Domains (2) summary

TIGR01517
Location:133 → 1047

ATPase-IIB_Ca; plasma-membrane calcium-translocating P-type ATPase

pfam12515
Location:30 → 71

CaATP_NAI; Ca2+-ATPase N terminal autoinhibitory domain
NM_001345242.1 → NP_001331281.1 autoinhibited Ca2+ -ATPase, isoform 8 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LF79, Q9LU75

UniProtKB/TrEMBL

A0A178UCR0, A0A384L942, B9DH83, Q0WV19

Conserved Domains (2) summary

TIGR01517
Location:133 → 1047

ATPase-IIB_Ca; plasma-membrane calcium-translocating P-type ATPase

pfam12515
Location:30 → 71

CaATP_NAI; Ca2+-ATPase N terminal autoinhibitory domain