Slc19a1 solute carrier family 19 member 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc19a1provided by RGD
Official Full Name: solute carrier family 19 member 1provided by RGD
Primary source: RGD:3695
See related: EnsemblRapid:ENSRNOG00000001232 AllianceGenome:RGD:3695
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: MTX1; MTX-1
Summary: Enables folate:monoatomic anion antiporter activity; folic acid binding activity; and methotrexate transmembrane transporter activity. Involved in several processes, including female pregnancy; methotrexate transport; and xenobiotic transmembrane transport. Located in apical plasma membrane; basolateral plasma membrane; and brush border membrane. Biomarker of chronic kidney disease. Human ortholog(s) of this gene implicated in several diseases, including abdominal aortic aneurysm; cleft lip; dextro-looped transposition of the great arteries; hematologic cancer; and malaria. Orthologous to human SLC19A1 (solute carrier family 19 member 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 109.9), Thymus (RPKM 46.5) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 20p12

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (11583928..11601959, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (11584410..11602429, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (12334675..12354517, complement)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Xanthones from securidaca inappendiculata antagonizes the anti-rheumatic effect of methotrexate by inhibiting reduced folate carrier 1.
Title: Xanthones from securidaca inappendiculata antagonizes the anti-rheumatic effect of methotrexate by inhibiting reduced folate carrier 1.
Role of Slc19a1 and Tfr2 in liver transport of iron and folate: A rat model of folate/iron deficiency followed by supplementation.
Title: Role of Slc19a1 and Tfr2 in liver transport of iron and folate: A rat model of folate/iron deficiency followed by supplementation.
Folate deficiency alters the expression of folate transporters RFC and folate exporter ABCG2 in the adult group differently than weanling group and young group both at the transcriptional and translational level, which in turn was found to be epigenetically regulated by promoter methylation of the RFC and ABCG2 genes.
Title: Gene specific epigenetic regulation of hepatic folate transport system is responsible for perturbed cellular folate status during aging and exogenous modulation.
decreased levels of PCFT and RFC expression in rat PPM are due to decreased association of these proteins with lipid rafts (LR) at the PPM
Title: Biochemical and molecular mechanisms of folate transport in rat pancreas; interference with ethanol ingestion.
Data suggest that malabsorption of folate in colon associated with alcoholism is due, in part, to down-regulation of folate transporter expression and association with lipid rafts (for both RFC/Slc19a1 and proton-coupled folate transporter/Slc46a1).
Title: Folate malabsorption is associated with down-regulation of folate transporter expression and function at colon basolateral membrane in rats.
Downregulation in the expression of the PCFT and the RFC in colon results in reduced levels of these transporters in colon apical membrane lipid rafts as a mechanism of folate malabsorption during chronic alcoholism.
Title: Reduced levels of folate transporters (PCFT and RFC) in membrane lipid rafts result in colonic folate malabsorption in chronic alcoholism.
Fusions of GFP with the shorter ORFs of all alternative slc19a1 transcripts containing 6-7 predicted TMD were expressed less efficiently and not sorted to the plasma membrane
Title: Alternative transcripts of rat slc19a1: defective sorting and inefficient expression of alternative splicing products--an addendum.
in IEC-6 cells, folate transport at neutral pH is mediated predominantly by RFC; however, the folate transport activity at pH 5.5 is RFC independent.
Title: Preservation of folate transport activity with a low-pH optimum in rat IEC-6 intestinal epithelial cell lines that lack reduced folate carrier function.
Three minor mRNA forms resulted from absent splicing of intron III, a shortened exon III, and a shortened exon IV. The minor transcripts were predicted to result in translation products with 7 or 6 instead of 12 transmembrane domains.
Title: Alternative transcripts of rat slc19a1: cloning, genomic organisation, tissue specific promoters and alternative splicing.
cloned and sequenced the renal sodium-dependent methotrexate carrier-1 and characterized its substrate spectrum
Title: Sodium-dependent methotrexate carrier-1 is expressed in rat kidney: cloning and functional characterization.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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Markers

U32469 (e-PCR)
- UniSTS:125722

RH129841 (e-PCR)
- UniSTS:213129

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 2',3'-cyclic GMP-AMP binding	ISO Inferred from Sequence Orthology more info
enables antiporter activity	ISO Inferred from Sequence Orthology more info
NOT enables cyclic-GMP-AMP transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables cyclic-GMP-AMP transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables folate:monoatomic anion antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables folate:monoatomic anion antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables folate:monoatomic anion antiporter activity	ISO Inferred from Sequence Orthology more info
enables folic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables folic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables folic acid transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables methotrexate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables methotrexate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables organic anion transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables vitamin transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables xenobiotic transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
NOT involved_in cyclic-GMP-AMP transmembrane import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in cyclic-GMP-AMP transmembrane import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in female pregnancy	IEP Inferred from Expression Pattern more info	PubMed
involved_in folate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in folate transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in folate transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in folic acid transport	ISO Inferred from Sequence Orthology more info
involved_in methotrexate transport	IDA Inferred from Direct Assay more info	PubMed
involved_in methotrexate transport	ISO Inferred from Sequence Orthology more info
involved_in monoatomic anion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in organic anion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cGAS/STING signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in response to toxic substance	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in vitamin transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in basolateral plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: reduced folate transporter

Names: RFC-1; RFC1; folate transporter 1; methotrexate carrier 1; methotrexate carrier 2; methotrexate carrier 5; methotrexate carrier 6; plasma membrane folate antiporter SLC19A1; reduced folate carrier 1; solute carrier family 19 (folate transporter), member 1; solute carrier family 19 (sodium/hydrogen exchanger), member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001035232.1 → NP_001030309.1 reduced folate transporter

See identical proteins and their annotated locations for NP_001030309.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a different segment for its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC085742

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
NM_017299.2 → NP_058995.2 reduced folate transporter

See identical proteins and their annotated locations for NP_058995.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AF099009, BC085742

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Related

ENSRNOP00000060579.3, ENSRNOT00000065236.5

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086038.1 Reference GRCr8

Range

11583928..11601959 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039098633.2 → XP_038954561.1 reduced folate transporter isoform X1

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_039098631.2 → XP_038954559.1 reduced folate transporter isoform X1

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_039098632.2 → XP_038954560.1 reduced folate transporter isoform X1

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_039098630.2 → XP_038954558.1 reduced folate transporter isoform X1

UniProtKB/Swiss-Prot

D3ZQS7, Q62866, Q9QUM8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_039098634.2 → XP_038954562.1 reduced folate transporter isoform X2

UniProtKB/TrEMBL

A6JKA5, Q5UD30, Q9QYB9

Conserved Domains (1) summary

cl27502
Location:1 → 377

Folate_carrier; Reduced folate carrier