REV3 recovery protein 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: REV3
Official Full Name: recovery protein 3
Primary source: TAIR:AT1G67500
Locus tag: AT1G67500
See related: Araport:AT1G67500
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATREV3; recovery protein 3; T1F15.3; T1F15_3
Summary: Encodes the catalytic subunit of DNA polymerase zeta.Mutants are sensitive to UV-B radiation. Gene is involved in damage-tolerance mechanisms through translesion synthesis(TLS).
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Genomic context

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See REV3 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 23

Sequence:: Chromosome: 1; NC_003070.9 (25287356..25296988, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that DNA polymerase zeta catalytic subunit REV3 cooperates with ATP-dependent DNA helicase RECQ4A and e ndonuclease MUS81 to repair DNA interstrand cross links.
Title: The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.
cooperation among DNA translesion synthesis (TLS) polymerases (Poleta, Polzeta) and DNA-damage-activated protein kinases (ATR, ATM)
Title: Cooperative responses of DNA-damage-activated protein kinases ATR and ATM and DNA translesion polymerases to replication-blocking DNA damage in a stem-cell niche.
The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed.
Title: Role of AtPolζ, AtRev1 and AtPolη in γ ray-induced mutagenesis.
suggesting that AtRAD5a and AtREV3 are required for error-free and translesion synthesis branches of DNA-damage tolerance, respectively
Title: RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis.
Proteins coded by AtREV3, AtREV1, and AtPOLH, are involved in TLS processes related to UV light mutagenesis. [REV3]
Title: Role of AtPolζ, AtRev1, and AtPolη in UV light-induced mutagenesis in Arabidopsis.
Results showed that crossing two lines homozygous for insertions in AtREV3 and AtPOLH and selfing F1 plants yielded non-Mendelian F2 genotype distributions: frequencies of +/++/+ and 1/1 2/2 progeny were only 0.42 and 0.25%.
Title: Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines.

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Pathways from PubChem

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General gene information

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Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA repair	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to UV-C	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to UV	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to UV-B	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translesion synthesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info
part_of zeta DNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of zeta DNA polymerase complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Preferred Names: recovery protein 3

NP_001319333.1

recovery protein 3 (REV3); CONTAINS InterPro DOMAIN/s: DNA polymerase, family B (InterPro:IPR022762), DNA-directed DNA polymerase, family B, exonuclease domain (InterPro:IPR006133), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337), DNA-directed DNA polymerase, family B, conserved site (InterPro:IPR017964), DNA-directed DNA polymerase, family B (InterPro:IPR006172), DNA-directed DNA polymerase, family B, conserved region (InterPro:IPR006134); BEST Arabidopsis thaliana protein match is: DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases (TAIR:AT5G63960.1).

NP_176917.2

recovery protein 3 (REV3); CONTAINS InterPro DOMAIN/s: DNA polymerase, family B (InterPro:IPR022762), DNA-directed DNA polymerase, family B, exonuclease domain (InterPro:IPR006133), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337), DNA-directed DNA polymerase, family B, conserved site (InterPro:IPR017964), DNA-directed DNA polymerase, family B (InterPro:IPR006172), DNA-directed DNA polymerase, family B, conserved region (InterPro:IPR006134); BEST Arabidopsis thaliana protein match is: DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases (TAIR:AT5G63960.1); Has 6179 Blast hits to 5524 proteins in 1389 species: Archae - 386; Bacteria - 883; Metazoa - 612; Fungi - 614; Plants - 201; Viruses - 2029; Other Eukaryotes - 1454 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

25287356..25296988 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001334302.1 → NP_001320390.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7B4, A0A1P8AMM8, A0A654ELU2
NM_001334303.1 → NP_001320389.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AMQ2, A0A7G2E3Y5
NM_105417.4 → NP_176917.2 recovery protein 3 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_176917.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O64795, Q766Z3, Q9CAG6

UniProtKB/TrEMBL

A0A178W7B4, A0A654ELU2

Conserved Domains (5) summary

cd05778
Location:1030 → 1277

DNA_polB_zeta_exo; inactive DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase zeta, a family-B DNA polymerase

cd05534
Location:1303 → 1751

POLBc_zeta; DNA polymerase type-B zeta subfamily catalytic domain. DNA polymerase (Pol) zeta is a member of the eukaryotic B-family of DNA polymerases and distantly related to DNA Pol delta. Pol zeta plays a major role in translesion replication and the production ...

smart00486
Location:1032 → 1533

POLBc; DNA polymerase type-B family

pfam00136
Location:1303 → 1743

DNA_pol_B; DNA polymerase family B

pfam14260
Location:1789 → 1862

zf-C4pol; C4-type zinc-finger of DNA polymerase delta
NM_001334301.1 → NP_001319333.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7B4, A0A654ELU2, F4HTM5
NM_001334304.1 → NP_001320393.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7B4, A0A1P8AMM8, A0A654ELU2
NM_001334305.1 → NP_001320391.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7B4, A0A1P8AMN7, A0A654ELU2
NM_001334306.1 → NP_001320392.1 recovery protein 3 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7B4, A0A654ELU2, F4HTM5