GCN2 protein kinase family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: GCN2
Official Full Name: protein kinase family protein
Primary source: TAIR:AT3G59410
Locus tag: AT3G59410
See related: Araport:AT3G59410
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA GENERAL CONTROL NON-REPRESSIBLE 2; ATGCN2; GENERAL CONTROL NON-REPRESSIBLE 2
Summary: Encodes an eIF2alpha kinase that can bind uncharged tRNA via its C-terminus and can phosphorylate both eIF2alpha homologues in Arabidopsis.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See GCN2 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 30

Sequence:: Chromosome: 3; NC_003074.8 (21948851..21959410)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eIF2alpha Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection.
Title: eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection.
Light Activates the Translational Regulatory Kinase GCN2 via Reactive Oxygen Species Emanating from the Chloroplast.
Title: Light Activates the Translational Regulatory Kinase GCN2 via Reactive Oxygen Species Emanating from the Chloroplast.
Arabidopsis GCN2 kinase regulates stomatal immunity by affecting pathogen-triggered stomatal closure and coronatine-mediated stomatal reopening.
Title: Arabidopsis GCN2 kinase contributes to ABA homeostasis and stomatal immunity.
Although both ILA and GCN2 genes are necessary to mediate eIF2alpha phosphorylation upon treatments with the aromatic amino acid biosynthesis inhibitor glyphosate, their mutants develop distinct root and chloroplast phenotypes.
Title: Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2α phosphorylation.
These results points to an unknown GCN2-dependent factor involved in the cascade of events triggered by glyphosate in plants.
Title: Protein kinase GCN2 mediates responses to glyphosate in Arabidopsis.
AtGCN2 plays pivotal roles in various cellular processes essential for normal growth and development.
Title: Characterization of Arabidopsis thaliana GCN2 kinase roles in seed germination and plant development.
The in vitro assays demonstrate the binding of the C-terminus of AtGCN2 to uncharged tRNA molecules and the enzymatic activities of AtGCN2 on both eIF2alpha homologues in A. thaliana.
Title: The GCN2 homologue in Arabidopsis thaliana interacts with uncharged tRNA and uses Arabidopsis eIF2α molecules as direct substrates.
GCN2 is activated following wounding and exposure to hormones. GCN2 can phosphorylate eIF2alpha and is essential in amino acid deprivation conditions.
Title: Arabidopsis eIF2alpha kinase GCN2 is essential for growth in stress conditions and is activated by wounding.
Analysis of wild-type and GT8359 (null mutant for AtGCN2) plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2alpha was not phosphorylated.
Title: GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2alpha in Arabidopsis.

Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_001339971.1

Supported by ESTs:

NM_115803.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables eukaryotic translation initiation factor 2alpha kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to amino acid starvation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of amino acid metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cytoplasmic translational initiation in response to stress	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within response to herbicide	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

Go to the top of the page Help

Preferred Names: protein kinase family protein

NP_001190135.1

GCN2; FUNCTIONS IN: protein serine/threonine kinase activity, protein tyrosine kinase activity, protein kinase activity, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: protein amino acid phosphorylation, translation; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Anticodon-binding (InterPro:IPR004154), Tyrosine-protein kinase, active site (InterPro:IPR008266), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), RWD (InterPro:IPR006575); BEST Arabidopsis thaliana protein match is: Class II aaRS and biotin synthetases superfamily protein (TAIR:AT3G02760.1).

NP_191500.2

GCN2; FUNCTIONS IN: protein tyrosine kinase activity, protein kinase activity, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: protein amino acid phosphorylation, translation; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Protein kinase, ATP binding site (InterPro:IPR017441), Protein kinase, catalytic domain (InterPro:IPR000719), Anticodon-binding (InterPro:IPR004154), Tyrosine-protein kinase, active site (InterPro:IPR008266), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), RWD (InterPro:IPR006575); BEST Arabidopsis thaliana protein match is: Class II aaRS and biotin synthetases superfamily protein (TAIR:AT3G02760.1); Has 102900 Blast hits to 101457 proteins in 4494 species: Archae - 105; Bacteria - 12314; Metazoa - 36099; Fungi - 10698; Plants - 26119; Viruses - 326; Other Eukaryotes - 17239 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

21948851..21959410

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001339969.1 → NP_001326041.1 protein kinase family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8H2D3, Q9LX30

UniProtKB/TrEMBL

A0A178VC30, A0A5S9XMZ6

Conserved Domains (3) summary

smart00591
Location:38 → 148

RWD; domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain

PLN02972
Location:796 → 1226

PLN02972; Histidyl-tRNA synthetase

cd14046
Location:418 → 729

STKc_EIF2AK4_GCN2_rpt2; Catalytic domain, repeat 2, of the Serine/Threonine kinase, eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or General Control Non-derepressible-2
NM_001339970.1 → NP_001326042.1 protein kinase family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1I9LN30, A0A654FJ76
NM_001203206.1 → NP_001190135.1 protein kinase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001190135.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178VC30, F4J8A5

Conserved Domains (6) summary

smart00220
Location:449 → 754

S_TKc; Serine/Threonine protein kinases, catalytic domain

smart00591
Location:68 → 172

RWD; domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain

COG0124
Location:818 → 1251

HisS; Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]

cd14046
Location:442 → 753

STKc_EIF2AK4_GCN2_rpt2; Catalytic domain, repeat 2, of the Serine/Threonine kinase, eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or General Control Non-derepressible-2

cl00266
Location:1156 → 1250

HGTP_anticodon; HGTP anticodon binding domain, as found at the C-terminus of histidyl, glycyl, threonyl and prolyl tRNA synthetases, which are classified as a group of class II aminoacyl-tRNA synthetases (aaRS). In aaRSs, the anticodon binding domain is responsible for ...

cl00268
Location:818 → 1145

class_II_aaRS-like_core; Class II tRNA amino-acyl synthetase-like catalytic core domain. Class II amino acyl-tRNA synthetases (aaRS) share a common fold and generally attach an amino acid to the 3' OH of ribose of the appropriate tRNA. PheRS is an exception in that it attaches ...
NM_115803.3 → NP_191500.2 protein kinase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_191500.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8H2D3, Q9LX30

UniProtKB/TrEMBL

A0A178VC30, A0A5S9XMZ6

Conserved Domains (3) summary

smart00591
Location:38 → 148

RWD; domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain

PLN02972
Location:796 → 1226

PLN02972; Histidyl-tRNA synthetase

cd14046
Location:418 → 729

STKc_EIF2AK4_GCN2_rpt2; Catalytic domain, repeat 2, of the Serine/Threonine kinase, eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or General Control Non-derepressible-2
NM_001339971.1 → NP_001326040.1 protein kinase family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178VC30, A0A1I9LN28