CalpB Calpain-B [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: CalpBprovided by FlyBase
Official Full Name: Calpain-Bprovided by FlyBase
Primary source: FLYBASE:FBgn0025866
Locus tag: Dmel_CG8107
See related: AllianceGenome:FB:FBgn0025866
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Calp B; calpB; CALPB; CG8107; Dmel\CG8107
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Involved in border follicle cell migration; neuron remodeling; and protein autoprocessing. Located in cytoplasm. Colocalizes with membrane. Is expressed in several structures, including adult head; dorsal pharyngeal muscle cell; embryonic/larval crystal cell; embryonic/larval esophageal visceral muscle; and larval muscle system. Used to study hereditary spastic paraplegia 76. Human ortholog(s) of this gene implicated in autosomal dominant limb-girdle muscular dystrophy; autosomal recessive limb-girdle muscular dystrophy type 2A; hereditary spastic paraplegia 76; and muscular dystrophy. Orthologous to several human genes including CAPN1 (calpain 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 67D1-67D1; 3-32 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (9888762..9893319)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (9881862..9886419)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study demonstrates that CalpB but not the structurally related CalpA has an important modulator function during in vivo cell migration.
Title: CalpB modulates border cell migration in Drosophila egg chambers.
Data show that mutations that disrupted endogenous calpainA or calpainB activity in transgenic flies suppressed tau toxicity.
Title: Implicating calpain in tau-mediated toxicity in vivo.
the activation of the ERK pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies.
Title: Regulation of calpain B from Drosophila melanogaster by phosphorylation.
CalpainB is required for AML1-ETO-induced blood cell disorders in Drosophila.
Title: A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO.
initial autolysis of Calpain B seems to occur intramolecularly, which supports the monomeric nature of Drosophila calpains
Title: Drosophila Calpain B is monomeric and autolyzes intramolecularly.
Autolytic activation, expression pattern and localization of calpain B.
Title: Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila.
the acidic loop and the transducer region of calpain form an interconnected, extended structural unit that has the capacity to integrate and transduce Ca2+-evoked conformational changes over a long distance
Title: Contribution of distinct structural elements to activation of calpain by Ca2+ ions.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	NAS Non-traceable Author Statement more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autoprocessing	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autoprocessing	TAS Traceable Author Statement more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	NAS Non-traceable Author Statement more info	PubMed
colocalizes_with membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Calpain-B

Names: CG8107-PA; CG8107-PB; CalpB-PA; CalpB-PB; calpain B; calpainB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

9888762..9893319

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079292.5 → NP_524016.4 Calpain-B, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524016.4

Status: REVIEWED

UniProtKB/Swiss-Prot

A9UN95, O96454, Q9VT65

UniProtKB/TrEMBL

M9NE73

Related

FBpp0076072

Conserved Domains (7) summary

COG5126
Location:827 → 908

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam00648
Location:260 → 556

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:574 → 721

Calpain_III; Calpain large subunit, domain III

pfam06346
Location:37 → 189

Drf_FH1; Formin Homology Region 1

pfam09310
Location:33 → 225

PD-C2-AF1; POU domain, class 2, associating factor 1

pfam13499
Location:759 → 825

EF-hand_7; EF-hand domain pair

cl08302
Location:761 → 826

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
NM_001259777.2 → NP_001246706.1 Calpain-B, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246706.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A9UN95, O96454, Q9VT65

UniProtKB/TrEMBL

M9NE73

Conserved Domains (7) summary

COG5126
Location:827 → 908

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam00648
Location:260 → 556

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:574 → 721

Calpain_III; Calpain large subunit, domain III

pfam06346
Location:37 → 189

Drf_FH1; Formin Homology Region 1

pfam09310
Location:33 → 225

PD-C2-AF1; POU domain, class 2, associating factor 1

pfam13499
Location:759 → 825

EF-hand_7; EF-hand domain pair

cl08302
Location:761 → 826

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...