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    Drep2 DNA fragmentation factor-related protein 2 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35955, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Drep2provided by FlyBase
    Official Full Name
    DNA fragmentation factor-related protein 2provided by FlyBase
    Primary source
    FLYBASE:FBgn0028408
    Locus tag
    Dmel_CG1975
    See related
    AllianceGenome:FB:FBgn0028408
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.162; anon-WO0118547.168; CG1975; Dmel\CG1975; Drep-2; DREP-2; drep2; DREP2; rep2; Rep2
    Summary
    Predicted to be involved in regulation of apoptotic process. Located in postsynaptic membrane. Is expressed in several structures, including adult head; adult optic lobe; brain; cuboidal/columnar epithelium; and synaptic neuropil. Human ortholog(s) of this gene implicated in familial partial lipodystrophy type 5 and obesity. Orthologous to human CIDEA (cell death inducing DFFA like effector a); CIDEB (cell death inducing DFFA like effector b); and CIDEC (cell death inducing DFFA like effector c). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
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    Genomic context

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    See Drep2 in Genome Data Viewer
    Location:
    45C4-45C5; 2-60 cM
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (9291856..9302821)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (5179361..5190326)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene antisense RNA:CR45132 Neighboring gene uncharacterized protein Neighboring gene Component of oligomeric golgi complex 6 Neighboring gene Mitotic arrest-deficient 1 Neighboring gene long non-coding RNA:CR45918 Neighboring gene Myd88 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene mir-987 stem loop

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification, crystallization and preliminary X-ray crystallographic studies of Drep2 CIDE domain. Lee SM, et al. Acta Crystallogr F Struct Biol Commun, 2014 Oct. PMID 25286952, Free PMC Article
    2. Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy. Lillvis JL, et al. Elife, 2022 Oct 26. PMID 36286237, Free PMC Article
    3. Crystallization and preliminary X-ray crystallographic studies of the CIDE-domain complex between Drep2 and Drep3 from Drosophila melanogaster. Park OK, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011 Apr 1. PMID 21505240, Free PMC Article
    4. CIDE domains form functionally important higher-order assemblies for DNA fragmentation. Choi JY, et al. Proc Natl Acad Sci U S A, 2017 Jul 11. PMID 28652364, Free PMC Article
    5. Crystal structure and mutation analysis revealed that DREP2 CIDE forms a filament-like structure with features differing from those of DREP4 CIDE. Ha HJ, et al. Sci Rep, 2018 Dec 13. PMID 30546036, Free PMC Article

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. the crystal structure and mutagenesis analysis of the DREP2 CIDE mutant, which confirmed that DREP2 CIDE also forms a filament-like structure with features differing from those of DREP4 CIDE.
      Title: Crystal structure and mutation analysis revealed that DREP2 CIDE forms a filament-like structure with features differing from those of DREP4 CIDE.
    2. Drep-2 is a novel regulatory synaptic factor, probably intersecting with metabotropic signaling and translational regulation.
      Title: Drep-2 is a novel synaptic protein important for learning and memory.
    3. study found that the interaction of Drep2 and Drep4 to Drep1 was not competitive indicating that Drep2 and Drep4 bind different place of Drep1
      Title: A putative role of Drep1 in apoptotic DNA fragmentation system in fly is mediated by direct interaction with Drep2 and Drep4.
    4. Data indicate that the basic surface side of Drep2 CIDE is involved in the interaction with the acidic surface side of Drep1 CIDE and Drep3 CIDE.
      Title: General interaction mode of CIDE:CIDE complex revealed by a mutation study of the Drep2 CIDE domain.
    5. Drep2 is a novel nuclease whose activity is inhibited by Drep3 through a tight interaction with the CIDE domain.
      Title: Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3.
    6. CIDE domains of Drep2 and Drep3 were purified in Escherichia coli, after which they formed a stable complex in vitro and were crystallized by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to a resolution of 5.8 A.
      Title: Crystallization and preliminary X-ray crystallographic studies of the CIDE-domain complex between Drep2 and Drep3 from Drosophila melanogaster.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    NOT enables nuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of apoptotic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in postsynaptic membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in synaptic membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA fragmentation factor-related protein 2
    Names
    CG1975-PA
    CG1975-PB
    CG1975-PC
    CG1975-PD
    Drep2-PA
    Drep2-PB
    Drep2-PC
    Drep2-PD
    Rep2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      9291856..9302821
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_136634.4 → NP_610478.1  DNA fragmentation factor-related protein 2, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_610478.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q7K304, Q9U787
      Related
      FBpp0087678
      Conserved Domains (1) summary
      pfam02017
      Location:9 → 85
      CIDE-N; CIDE-N domain

    2. NM_001259293.2 → NP_001246222.1  DNA fragmentation factor-related protein 2, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246222.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q7K304, Q9U787
      Conserved Domains (1) summary
      pfam02017
      Location:9 → 85
      CIDE-N; CIDE-N domain

    3. NM_001273891.1 → NP_001260820.1  DNA fragmentation factor-related protein 2, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260820.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4KFF6, F3YD50
      Conserved Domains (1) summary
      pfam02017
      Location:24 → 100
      CIDE-N; CIDE-N domain

    4. NM_001103768.3 → NP_001097238.1  DNA fragmentation factor-related protein 2, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097238.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8DY77, Q9U787
      Conserved Domains (1) summary
      pfam02017
      Location:76 → 152
      CIDE-N; CIDE-N domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4KFF6 A0A0B4KFF6
    A8DY77 A8DY77
    Q7K304 Q7K304
    Q95S67 Q95S67
    Q9U787 Q9U787

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