REV1 DNA-directed DNA polymerase [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: REV1
Official Full Name: DNA-directed DNA polymerase
Primary source: TAIR:AT5G44750
Locus tag: AT5G44750
See related: Araport:AT5G44750
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA HOMOLOG OF REVERSIONLESS 1; ATREV1; K23L20.9; K23L20_9
Summary: Homologous to Y-family DNA polymerases, contains BRCT domain. Mutants are sensitive to UV-B radiation. Gene is involved in damage-tolerance mechanisms through translesion synthesis(TLS).
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Genomic context

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See REV1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 24

Sequence:: Chromosome: 5; NC_003076.8 (18052607..18060169)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed.
Title: Role of AtPolζ, AtRev1 and AtPolη in γ ray-induced mutagenesis.
Arabidopsis thaliana disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode Translesion synthesis-type polymerases.
Title: Role of AtPolζ, AtRev1, and AtPolη in UV light-induced mutagenesis in Arabidopsis.
Transgenic plants that over-expressed or disrupted REV1 showed reduced germination percentage, but the former exhibited a higher stem growth rate than the wild type during development.
Title: Two translesion synthesis DNA polymerase genes, AtPOLH and AtREV1, are involved in development and UV light resistance in Arabidopsis.
A study was conducted to clarify the function of REV1 by in vitro analysis using a primer extension assay.
Title: AtREV1, a Y-family DNA polymerase in Arabidopsis, has deoxynucleotidyl transferase activity in vitro.
A translesion synthesis mechanism exists in higher plants and AtREV1 and AtREV7 have important roles in tolerating exposure to DNA-damaging agents. [AtREV1]
Title: Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis.

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Pathways from PubChem

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General gene information

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NM_001125901.2

Supported by ESTs:

NM_123842.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-prone translesion synthesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to UV-B	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: DNA-directed DNA polymerase

NP_001119373.1

REV1; FUNCTIONS IN: DNA-directed DNA polymerase activity; INVOLVED IN: DNA repair, response to UV-B, response to DNA damage stimulus; LOCATED IN: intracellular, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DNA polymerase, Y-family, little finger domain (InterPro:IPR017961), DNA-repair protein, UmuC-like (InterPro:IPR001126), DNA-repair protein, UmuC-like, N-terminal (InterPro:IPR017963), DNA repair protein, Rev1 (InterPro:IPR012112), BRCT (InterPro:IPR001357); BEST Arabidopsis thaliana protein match is: DNA/RNA polymerases superfamily protein (TAIR:AT1G49980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_199288.4

REV1; FUNCTIONS IN: DNA-directed DNA polymerase activity; INVOLVED IN: DNA repair, response to UV-B, response to DNA damage stimulus; LOCATED IN: intracellular, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DNA polymerase, Y-family, little finger domain (InterPro:IPR017961), DNA-repair protein, UmuC-like (InterPro:IPR001126), DNA-repair protein, UmuC-like, N-terminal (InterPro:IPR017963), DNA repair protein, Rev1 (InterPro:IPR012112), BRCT (InterPro:IPR001357); BEST Arabidopsis thaliana protein match is: DNA/RNA polymerases superfamily protein (TAIR:AT1G49980.1); Has 10364 Blast hits to 10129 proteins in 2421 species: Archae - 210; Bacteria - 6656; Metazoa - 678; Fungi - 378; Plants - 159; Viruses - 3; Other Eukaryotes - 2280 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

18052607..18060169

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001125901.2 → NP_001119373.1 DNA-directed DNA polymerase [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001119373.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A3EWL3, A3EWL4, A3EWL5, A3EWL6, A3EWL7, A3EWL8, O48585, Q5FBB9, Q8RXL1

UniProtKB/TrEMBL

A0A654G8Y2

Conserved Domains (3) summary

cd01701
Location:326 → 730

PolY_Rev1; DNA polymerase Rev1

smart00292
Location:87 → 161

BRCT; breast cancer carboxy-terminal domain

COG0389
Location:375 → 734

DinP; Nucleotidyltransferase/DNA polymerase involved in DNA repair [Replication, recombination and repair]
NM_123842.5 → NP_199288.4 DNA-directed DNA polymerase [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_199288.4

Status: REVIEWED

UniProtKB/TrEMBL

A0A654G8Y2

Conserved Domains (3) summary

cd01701
Location:326 → 726

PolY_Rev1; DNA polymerase Rev1

smart00292
Location:87 → 161

BRCT; breast cancer carboxy-terminal domain

COG0389
Location:375 → 730

DinP; Nucleotidyltransferase/DNA polymerase involved in DNA repair [Replication, recombination and repair]