Dao D-amino acid oxidase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Daoprovided by MGI
Official Full Name: D-amino acid oxidaseprovided by MGI
Primary source: MGI:MGI:94859
See related: Ensembl:ENSMUSG00000042096 AllianceGenome:MGI:94859
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DAAO; Dao1; DAMOX; Dao-1
Summary: Enables D-amino-acid dehydrogenase activity; D-amino-acid oxidase activity; and FAD binding activity. Involved in several processes, including D-alanine catabolic process; dopamine biosynthetic process; and neutrophil-mediated killing of gram-negative bacterium. Acts upstream of or within L-leucine metabolic process. Located in extracellular space and peroxisomal matrix. Is expressed in metanephros; renal cortex; ureter; and urinary system. Human ortholog(s) of this gene implicated in schizophrenia. Orthologous to human DAO (D-amino acid oxidase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in kidney adult (RPKM 130.9), duodenum adult (RPKM 16.0) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 55.93 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (114137696..114163736)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (113999635..114025675)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Factors regulating serine racemase and d-amino acid oxidase expression in the mouse striatum.
Title: Factors regulating serine racemase and d-amino acid oxidase expression in the mouse striatum.
Study demonstrates that robust and selective demethylation of two CpG sites is associated with postnatal activation of the Dao gene and consequent removal of D-serine within the cerebellum.
Title: Selective demethylation of two CpG sites causes postnatal activation of the Dao gene and consequent removal of D-serine within the mouse cerebellum.
We show that prolonged treatment with selegiline fails to reduce aberrant astrocytic GABA levels and rescue memory impairment in APP/PS1 mice, an animal model of AD, because of increased activity in compensatory genes for a GABA-synthesizing enzyme, diamine oxidase (DAO)
Title: Newly developed reversible MAO-B inhibitor circumvents the shortcomings of irreversible inhibitors in Alzheimer's disease.
age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues: implication for aging and neurodegeneration
Title: Age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues: implication for aging and neurodegeneration.
This work provides the most complete baseline analysis of L- and D-amino acids in the brains of wild-type and mutant mice lacking D-amino acid oxidase activity.
Title: Variations of L- and D-amino acid levels in the brain of wild-type and mutant mice lacking D-amino acid oxidase activity.
findings reveal that DAO is a microbiota-regulated host innate immune factor that modulates growth of both pathogens and commensals, primarily in the small intestine
Title: Interplay between microbial d-amino acids and host d-amino acid oxidase modifies murine mucosal defence and gut microbiota.
some significant in vivo effects of DAOR199W on motor neuron function (i.e. kyphosis and loss of motor neurons) were detected which were most marked in females and could contribute to the earlier onset of neurological signs in double transgenic females compared to SOD1G93A littermates, highlighting the importance of recognizing gender effects present in animal models of ALS
Title: Characterisation of the pathogenic effects of the in vivo expression of an ALS-linked mutation in D-amino acid oxidase: Phenotype and loss of spinal cord motor neurons.
Long-term spatial memory is unaltered in Dao-/- mice.
Title: Searching for cognitive enhancement in the Morris water maze: better and worse performance in D-amino acid oxidase knockout (Dao(-/-)) mice.
Dao-/- mice demonstrated enhanced spatial recognition memory and no sleep or circadian rhythm disruption.
Title: d-amino acid oxidase knockout (Dao(-/-) ) mice show enhanced short-term memory performance and heightened anxiety, but no sleep or circadian rhythm disruption.
In d-Amino acid oxidase (-/-) mice, approximately twice as many Dopamine-like neurons fired in a bursting pattern than in DAO(+/-) or DAO(+/+) mice. Data provide the first direct evidence that DAO modulates VTA DA neuron activity
Title: Increased burst-firing of ventral tegmental area dopaminergic neurons in D-amino acid oxidase knockout mice in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Spontaneous (1) 1 citation
Targeted (1) 1 citation

General gene information

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Markers

AI987963 (e-PCR)
- UniSTS:165421

D10Chm57 (e-PCR)
- UniSTS:518320

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables D-amino-acid dehydrogenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables D-amino-acid oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables D-amino-acid oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables D-amino-acid oxidase activity	ISO Inferred from Sequence Orthology more info
enables FAD binding	IDA Inferred from Direct Assay more info	PubMed
enables FAD binding	ISO Inferred from Sequence Orthology more info
enables glycine oxidase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in D-alanine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in D-alanine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in D-alanine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in D-amino acid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in D-serine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in D-serine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in D-serine metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within L-leucine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in digestion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in killing by host of symbiont cells	IDA Inferred from Direct Assay more info	PubMed
involved_in neutrophil-mediated killing of gram-negative bacterium	IDA Inferred from Direct Assay more info	PubMed
involved_in neutrophil-mediated killing of gram-negative bacterium	ISS Inferred from Sequence or Structural Similarity more info
involved_in proline catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proline catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in peroxisomal matrix	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal matrix	ISO Inferred from Sequence Orthology more info
located_in peroxisome	ISS Inferred from Sequence or Structural Similarity more info
located_in presynaptic active zone	ISO Inferred from Sequence Orthology more info
located_in presynaptic active zone	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: D-amino-acid oxidase

Names: D-amino acid oxidase 1

NP_001273325.1

EC 1.4.3.3

NP_001273326.1

EC 1.4.3.3

NP_034148.2

EC 1.4.3.3

XP_006530224.1

EC 1.4.3.3

XP_006530225.1

EC 1.4.3.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001286396.1 → NP_001273325.1 D-amino-acid oxidase isoform 1

See identical proteins and their annotated locations for NP_001273325.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

Source sequence(s)

AC145559

Consensus CDS

CCDS19557.1

UniProtKB/Swiss-Prot

A0A0R4J203, P18894, Q64465, Q8VCW7

Related

ENSMUSP00000125588.2, ENSMUST00000161610.6

Conserved Domains (2) summary

pfam01266
Location:35 → 327

DAO; FAD dependent oxidoreductase

cl21454
Location:1 → 24

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins
NM_001286397.1 → NP_001273326.1 D-amino-acid oxidase isoform 2

See identical proteins and their annotated locations for NP_001273326.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC145559

Consensus CDS

CCDS71641.1

UniProtKB/TrEMBL

Q91WH3

Related

ENSMUSP00000083792.5, ENSMUST00000086599.11

Conserved Domains (1) summary

pfam01266
Location:2 → 226

DAO; FAD dependent oxidoreductase
NM_010018.3 → NP_034148.2 D-amino-acid oxidase isoform 1

See identical proteins and their annotated locations for NP_034148.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Variants 1 and 2 encode the same protein.

Source sequence(s)

AC145559, AK134813, AW492600, BU755879

Consensus CDS

CCDS19557.1

UniProtKB/Swiss-Prot

A0A0R4J203, P18894, Q64465, Q8VCW7

Related

ENSMUSP00000107911.3, ENSMUST00000112292.9

Conserved Domains (2) summary

pfam01266
Location:35 → 327

DAO; FAD dependent oxidoreductase

cl21454
Location:1 → 24

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

114137696..114163736

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006530162.4 → XP_006530225.1 D-amino-acid oxidase isoform X1

See identical proteins and their annotated locations for XP_006530225.1

UniProtKB/Swiss-Prot

A0A0R4J203, P18894, Q64465, Q8VCW7

Conserved Domains (2) summary

pfam01266
Location:35 → 327

DAO; FAD dependent oxidoreductase

cl21454
Location:1 → 24

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins
XM_006530161.2 → XP_006530224.1 D-amino-acid oxidase isoform X1

See identical proteins and their annotated locations for XP_006530224.1

UniProtKB/Swiss-Prot

A0A0R4J203, P18894, Q64465, Q8VCW7

Conserved Domains (2) summary

pfam01266
Location:35 → 327

DAO; FAD dependent oxidoreductase

cl21454
Location:1 → 24

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins