Rab34 RAB34, member RAS oncogene family [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rab34provided by MGI
Official Full Name: RAB34, member RAS oncogene familyprovided by MGI
Primary source: MGI:MGI:104606
See related: Ensembl:ENSMUSG00000002059 AllianceGenome:MGI:104606
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rah; Narr; Rah1
Summary: Enables GTPase activity and guanyl nucleotide binding activity. Involved in several processes, including Golgi to plasma membrane protein transport; organelle assembly; and positive regulation of protein localization to phagocytic vesicle. Acts upstream of or within endocytosis. Located in Golgi apparatus; endosome; and ruffle. Colocalizes with phagocytic vesicle. Is expressed in several structures, including adrenal gland; alimentary system; brain; genitourinary system; and respiratory system. Human ortholog(s) of this gene implicated in orofaciodigital syndrome XX. Orthologous to human RAB34 (RAB34, member RAS oncogene family). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in adrenal adult (RPKM 68.8), ovary adult (RPKM 45.7) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 46.74 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (78079253..78083019)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (78188427..78192193)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.
Title: Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.
Rab34 is necessary for early stages of intracellular ciliogenesis.
Title: Rab34 is necessary for early stages of intracellular ciliogenesis.
Authors demonstrate that endocytic recycling protein Rab34 localizes to cilia, and that its mutation results in significant decrease of ciliogenesis in both cultured cells and mice. Rab34 is required for the successive fusion of preciliary vesicles to generate ciliary vesicles and for the migration of the mother centriole from perinuclear region to plasma membrane.
Title: Rab34 small GTPase is required for Hedgehog signaling and an early step of ciliary vesicle formation in mouse.
CSN6 and Rab34 are involved in AR trafficking by regulating the phosphorylation signaling pathway. These findings provide new insights into the testosterone signaling pathway in Sertoli cells that mediates spermatogenesis.
Title: CSN6 and Rab34 Are Involved in Androgen Receptor Trafficking in Mouse Testicular Sertoli Cells.
Loss-of-function mutations in the Rab34 gene lead to defects in the assembly of primary cilia, in the trafficking of proteins to primary cilia and, consequently, to impaired reception of signals from the Hedgehog pathway.
Title: CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
Although Rab34 regulates both lysosomal positioning and lysosome to phagosome fusion, the latter effect is not due to the regulation of the cytoplasmic accessibility of lysosomes to phagosomes by Rab34.
Title: Spatial distribution of phagolysosomes is independent of the regulation of lysosome position by Rab34.
uncover Rab34/Munc13-2 as a critical part of an alternative Rab7-independent phagosome maturation machinery and lysosome-mediated killing of mycobacteria
Title: Size-dependent mechanism of cargo sorting during lysosome-phagosome fusion is controlled by Rab34.
association with membrane ruffles and macropinosomes and promotes macropinosome formation
Title: Small GTPase Rah/Rab34 is associated with membrane ruffles and macropinosomes and promotes macropinosome formation.
results have uncovered a novel mechanism for interorganellar trans-regulation of spatial distribution of lysosomes by Golgi-associated Rab34
Title: Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI326479 (e-PCR)
- UniSTS:164470

D11Seg7 (e-PCR), detects polymorphism
- UniSTS:141823

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTP-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl nucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi to plasma membrane protein transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Golgi to plasma membrane protein transport	ISO Inferred from Sequence Orthology more info
involved_in antigen processing and presentation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in cilium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in cytosolic ciliogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in lysosomal transport	IDA Inferred from Direct Assay more info	PubMed
involved_in lysosome localization	ISO Inferred from Sequence Orthology more info
involved_in phagosome maturation	ISO Inferred from Sequence Orthology more info
involved_in phagosome maturation	ISS Inferred from Sequence or Structural Similarity more info
involved_in phagosome-lysosome fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in phagosome-lysosome fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phagosome-lysosome fusion	ISO Inferred from Sequence Orthology more info
involved_in phagosome-lysosome fusion	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of defense response to bacterium	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein localization to phagocytic vesicle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in Golgi cisterna	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi cisterna	ISO Inferred from Sequence Orthology more info
located_in Golgi stack	ISO Inferred from Sequence Orthology more info
located_in centriole	ISO Inferred from Sequence Orthology more info
located_in centriole	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in cilium	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
colocalizes_with phagocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in phagocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in phagocytic vesicle	ISS Inferred from Sequence or Structural Similarity more info
located_in phagocytic vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ras-related protein Rab-34

Names: RAB34, member of RAS oncogene family; rab-39; ras-related homolog; ras-related protein Rab-39; ras-related protein Rah

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001159482.1 → NP_001152954.1 ras-related protein Rab-34

See identical proteins and their annotated locations for NP_001152954.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AA041780, AB082927, AF327929, AV142766, BY317641

Consensus CDS

CCDS25094.1

UniProtKB/Swiss-Prot

Q64008, Q8BHJ0, Q99P59, Q99P90

UniProtKB/TrEMBL

Q0PD20

Related

ENSMUSP00000002128.8, ENSMUST00000002128.14

Conserved Domains (2) summary

smart00175
Location:54 → 213

RAB; Rab subfamily of small GTPases

cd04108
Location:53 → 220

Rab36_Rab34; Rab GTPase families 34 (Rab34) and 36 (Rab36)
NM_033475.3 → NP_258436.2 ras-related protein Rab-34

See identical proteins and their annotated locations for NP_258436.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AB082927, AV142766, BB643883, BY195955, BY234941

Consensus CDS

CCDS25094.1

UniProtKB/Swiss-Prot

Q64008, Q8BHJ0, Q99P59, Q99P90

UniProtKB/TrEMBL

Q0PD20

Related

ENSMUSP00000103958.3, ENSMUST00000108322.9

Conserved Domains (2) summary

smart00175
Location:54 → 213

RAB; Rab subfamily of small GTPases

cd04108
Location:53 → 220

Rab36_Rab34; Rab GTPase families 34 (Rab34) and 36 (Rab36)