Chrna3 cholinergic receptor nicotinic alpha 3 subunit [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chrna3provided by RGD
Official Full Name: cholinergic receptor nicotinic alpha 3 subunitprovided by RGD
Primary source: RGD:2345
See related: EnsemblRapid:ENSRNOG00000013829 AllianceGenome:RGD:2345
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables acetylcholine binding activity; heterocyclic compound binding activity; and postsynaptic neurotransmitter receptor activity. Involved in several processes, including acetylcholine receptor signaling pathway; presynaptic modulation of chemical synaptic transmission; and response to carbon monoxide. Located in dendrite; neuronal cell body; and plasma membrane raft. Part of acetylcholine-gated channel complex. Is active in cholinergic synapse; dopaminergic synapse; and postsynaptic specialization membrane. Biomarker of depressive disorder. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; chronic obstructive pulmonary disease; cocaine dependence; lung cancer (multiple); and nicotine dependence. Orthologous to human CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 21.6), Spleen (RPKM 9.3) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (64297755..64311251, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (55401668..55415165, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (59594007..59607122, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

alpha3* Nicotinic Acetylcholine Receptors in the Habenula-Interpeduncular Nucleus Circuit Regulate Nicotine Intake.
Title: α3* Nicotinic Acetylcholine Receptors in the Habenula-Interpeduncular Nucleus Circuit Regulate Nicotine Intake.
Results suggest the fast nAChR current in cutaneous dorsal root ganglia neurons was carried by homomeric alpha7 nAChRs while the slow current was carried by heteromeric (most likely alpha3beta4) nAChRs
Title: Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.
The sympathetic neuronal calcium influx through L-type calcium channels is modulated by alpha3beta2-nAChRs
Title: L-type calcium channels in sympathetic α3β2-nAChR-mediated cerebral nitrergic neurogenic vasodilation.
Data indicate that the affinity of alpha-CTxLvIA selectively blocks alpha3beta2 vs. alpha6/alpha3beta2beta3 nicotinic acetylcholine receptors.
Title: A novel α4/7-conotoxin LvIA from Conus lividus that selectively blocks α3β2 vs. α6/α3β2β3 nicotinic acetylcholine receptors.
Positional scanning mutagenesis of alpha-conotoxin PeIA identifies critical residues that confer potency and selectivity for alpha6/alpha3beta2beta3 and alpha3beta2 nicotinic acetylcholine receptors
Title: Positional scanning mutagenesis of α-conotoxin PeIA identifies critical residues that confer potency and selectivity for α6/α3β2β3 and α3β2 nicotinic acetylcholine receptors.
determined the site specificity for Mor potentiation by studying differences in modulation between alpha3beta2 and alpha4beta2 receptors
Title: Specificity determinants of allosteric modulation in the neuronal nicotinic acetylcholine receptor: a fine line between inhibition and potentiation.
These studies identify alpha3beta4 and alpha3beta3beta4, transported from the Hb and highly enriched in the IPn, as the subtypes modulating ACh release in the IPn.
Title: Rodent habenulo-interpeduncular pathway expresses a large variety of uncommon nAChR subtypes, but only the alpha3beta4* and alpha3beta3beta4* subtypes mediate acetylcholine release.
side chain volume at position L8 affected ion channel function and the pharmacological profile of neuronal 3 nAChR subtypes.
Title: Functional contribution of alpha3L8' to the neuronal nicotinic alpha3 receptor.
The role of negatively charged amino acids in the F-loop of the beta 4 subunit in channel activation and desensitization was studied using the patch-clamp technique.
Title: Role of negatively charged amino acids in beta 4 F-loop in activation and desensitization of alpha 3 beta 4 rat neuronal nicotinic receptors.
In living cells the heteropentameric alpha3-beta4 nicotinic receptors are mainly distributed in the endoplasmic reticulum.
Title: Distinct subcellular targeting of fluorescent nicotinic alpha 3 beta 4 and serotoninergic 5-HT3A receptors in hippocampal neurons.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:261122

PMC312758P4 (e-PCR)
- UniSTS:272915

D8Bord1 (e-PCR)
- UniSTS:239636

D8Wox30 (e-PCR)
- UniSTS:518685

BE108721 (e-PCR)
- UniSTS:247872

Chrna3 (e-PCR), detects polymorphism
- UniSTS:256423

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:256428

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acetylcholine binding	IDA Inferred from Direct Assay more info	PubMed
enables acetylcholine receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables acetylcholine receptor activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IGI Inferred from Genetic Interaction more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	IDA Inferred from Direct Assay more info	PubMed
enables heterocyclic compound binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within activation of transmembrane receptor protein tyrosine kinase activity	ISO Inferred from Sequence Orthology more info
involved_in behavioral response to nicotine	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within excitatory postsynaptic potential	ISO Inferred from Sequence Orthology more info
involved_in heart development	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in nervous system development	ISO Inferred from Sequence Orthology more info
involved_in neuromuscular synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in presynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of acetylcholine secretion, neurotransmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of membrane potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in response to acetylcholine	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to carbon monoxide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nicotine	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to nicotine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission involved in micturition	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, cholinergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, cholinergic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within synaptic transmission, cholinergic	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IDA Inferred from Direct Assay more info	PubMed
part_of acetylcholine-gated channel complex	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
part_of acetylcholine-gated channel complex	TAS Traceable Author Statement more info	PubMed
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
is_active_in dopaminergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in dopaminergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	IGI Inferred from Genetic Interaction more info	PubMed
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit alpha-3

Names: Acetylcholine receptor alpha 3 (neuronal nicotine); acetylcholine receptor alpha3; cetylcholine receptor alpha 3 (neuronal nicotine); cholinergic receptor nicotinic alpha polypeptide 4; cholinergic receptor, nicotinic, alpha 3 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_052805.3 → NP_434692.2 neuronal acetylcholine receptor subunit alpha-3 precursor

See identical proteins and their annotated locations for NP_434692.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000008

UniProtKB/Swiss-Prot

P04757

UniProtKB/TrEMBL

F1M7K6, Q6PW51

Related

ENSRNOP00000019307.4, ENSRNOT00000019307.6

Conserved Domains (3) summary

TIGR00860
Location:24 → 490

LIC; Cation transporter family protein

pfam02931
Location:29 → 235

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:242 → 489

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region