Mical1 microtubule associated monooxygenase, calponin and LIM domain containing 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mical1provided by MGI
Official Full Name: microtubule associated monooxygenase, calponin and LIM domain containing 1provided by MGI
Primary source: MGI:MGI:2385847
See related: Ensembl:ENSMUSG00000019823 AllianceGenome:MGI:2385847
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MICAL; Nical; MICAL-1
Summary: Enables several functions, including FAD binding activity; actin binding activity; and enzyme binding activity. Involved in several processes, including actin filament depolymerization; negative regulation of protein metabolic process; and sulfur oxidation. Located in hippocampal mossy fiber expansion. Orthologous to human MICAL1 (microtubule associated monooxygenase, calponin and LIM domain containing 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in duodenum adult (RPKM 45.4), thymus adult (RPKM 34.0) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B1; 10 22.41 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (41351693..41363026)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (41475860..41487030)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mical1 deletion in tyrosinase expressing cells affects mouse running gaits.
Title: Mical1 deletion in tyrosinase expressing cells affects mouse running gaits.
MICAL1 (molecule interacting with CasL 1) protects oligodendrocyte cells from oxidative injury through regulating apoptosis, autophagy in spinal cord injury.
Title: MICAL1 (molecule interacting with CasL 1) protects oligodendrocyte cells from oxidative injury through regulating apoptosis, autophagy in spinal cord injury.
MICAL1 constrains cardiac stress responses and protects against disease by oxidizing CaMKII.
Title: MICAL1 constrains cardiac stress responses and protects against disease by oxidizing CaMKII.
esults suggest that the CH domain, which is loosely connected to the MO domain by a flexible linker and is far away from the catalytic site, couples F-actin to the enhancement of redox activity of MICALMO-CH by a cooperative mechanism involving a trans interaction between adjacently bound molecules.
Title: Modulation of MICAL Monooxygenase Activity by its Calponin Homology Domain: Structural and Mechanistic Insights.
MICAL-1 regulates the targeting of secretory vesicles containing immunoglobulin superfamily cell adhesion molecules (IgCAMs) to the neuronal growth cone membrane through its ability to control the actin cytoskeleton using redox chemistry.
Title: The intracellular redox protein MICAL-1 regulates the development of hippocampal mossy fibre connections.
Findings show a biological role for MICAL-1 in apoptosis and define a novel negative regulatory mechanism of MST-NDR signaling.
Title: MICAL-1 is a negative regulator of MST-NDR kinase signaling and apoptosis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables F-actin monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables FAD binding	IDA Inferred from Direct Assay more info	PubMed
enables FAD binding	ISO Inferred from Sequence Orthology more info
enables FAD binding	ISS Inferred from Sequence or Structural Similarity more info
enables NAD(P)H oxidase H2O2-forming activity	ISO Inferred from Sequence Orthology more info
enables NADPH oxidase H202-forming activity	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	ISO Inferred from Sequence Orthology more info
enables actin binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables monooxygenase activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	IDA Inferred from Direct Assay more info	PubMed
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament depolymerization	ISO Inferred from Sequence Orthology more info
involved_in actin filament depolymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of regulated secretory pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sulfur oxidation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in actin filament	IEA Inferred from Electronic Annotation more info
is_active_in actin filament	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in endosome membrane	ISO Inferred from Sequence Orthology more info
located_in endosome membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in hippocampal mossy fiber expansion	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intercellular bridge	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in midbody	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: [F-actin]-monooxygenase MICAL1; F-actin-monooxygenase MICAL1

Names: CasL interacting molecule MICAL; NEDD9-interacting protein with calponin homology and LIM domains; [F-actin]-methionine sulfoxide oxidase MICAL1; microtubule associated monoxygenase, calponin and LIM domain containing 1; molecule interacting with CasL protein 1; protein-methionine sulfoxide oxidase MICAL1

NP_001157905.1

EC 1.14.13.225

EC 1.6.3.1

NP_001345767.1

EC 1.14.13.225

EC 1.6.3.1

NP_001345768.1

EC 1.14.13.225

EC 1.6.3.1

NP_612188.1

EC 1.14.13.225

EC 1.6.3.1

XP_006512633.1

EC 1.14.13.225

EC 1.6.3.1

XP_006512637.1

EC 1.14.13.225

EC 1.6.3.1

XP_011241439.1

EC 1.14.13.225

EC 1.6.3.1

XP_017169310.1

EC 1.14.13.225

EC 1.6.3.1

XP_030100786.1

EC 1.14.13.225

EC 1.6.3.1

XP_030100787.1

EC 1.14.13.225

EC 1.6.3.1

XP_030100788.1

EC 1.14.13.225

EC 1.6.3.1

XP_036011548.1

EC 1.14.13.225

EC 1.6.3.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164433.1 → NP_001157905.1 [F-actin]-monooxygenase MICAL1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

Source sequence(s)

AC153360, AK159369, AK171411, AK171429

Consensus CDS

CCDS48547.1

UniProtKB/TrEMBL

E9PUI4

Related

ENSMUSP00000097519.5, ENSMUST00000099934.11

Conserved Domains (3) summary

cd09358
Location:610 → 663

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam00307
Location:438 → 533

CH; Calponin homology (CH) domain

pfam12130
Location:841 → 973

DUF3585; Protein of unknown function (DUF3585)
NM_001358838.1 → NP_001345767.1 [F-actin]-monooxygenase MICAL1 isoform 3

Status: VALIDATED

Source sequence(s)

AC153360, AK159369, AK171429, BE691989, BY210647, CV562084

Conserved Domains (3) summary

cd09358
Location:498 → 551

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam12130
Location:733 → 861

DUF3585; Protein of unknown function (DUF3585)

cd21196
Location:321 → 426

CH_MICAL1; calponin homology (CH) domain found in molecule interacting with CasL protein 1
NM_001358839.1 → NP_001345768.1 [F-actin]-monooxygenase MICAL1 isoform 4

Status: VALIDATED

Source sequence(s)

AC153360, AK159369, AK171429, BE691989, CA528494

Conserved Domains (3) summary

cd09358
Location:452 → 505

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam00307
Location:280 → 380

CH; Calponin homology (CH) domain

pfam12130
Location:687 → 815

DUF3585; Protein of unknown function (DUF3585)
NM_138315.2 → NP_612188.1 [F-actin]-monooxygenase MICAL1 isoform 1

See identical proteins and their annotated locations for NP_612188.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC153360, AK171411, AK171429

Consensus CDS

CCDS35888.1

UniProtKB/Swiss-Prot

D3Z4C6, E9PXR1, Q3TB77, Q3TBH9, Q3TX89, Q8VDP3

UniProtKB/TrEMBL

E9PUI4

Related

ENSMUSP00000019967.10, ENSMUST00000019967.16

Conserved Domains (5) summary

cd09358
Location:683 → 736

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

COG2081
Location:88 → 224

YhiN; Predicted flavoprotein YhiN [General function prediction only]

pfam00307
Location:511 → 611

CH; Calponin homology (CH) domain

pfam01494
Location:85 → 122

FAD_binding_3; FAD binding domain

pfam12130
Location:918 → 1046

DUF3585; Protein of unknown function (DUF3585)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

41351693..41363026

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030244926.2 → XP_030100786.1 F-actin-monooxygenase MICAL1 isoform X3

Conserved Domains (3) summary

cd09358
Location:498 → 551

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam12130
Location:733 → 861

DUF3585; Protein of unknown function (DUF3585)

cd21196
Location:321 → 426

CH_MICAL1; calponin homology (CH) domain found in molecule interacting with CasL protein 1
XM_006512570.2 → XP_006512633.1 F-actin-monooxygenase MICAL1 isoform X1

Conserved Domains (4) summary

cd09358
Location:842 → 895

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam00307
Location:670 → 770

CH; Calponin homology (CH) domain

pfam01494
Location:244 → 281

FAD_binding_3; FAD binding domain

pfam12130
Location:1077 → 1205

DUF3585; Protein of unknown function (DUF3585)
XM_030244927.2 → XP_030100787.1 F-actin-monooxygenase MICAL1 isoform X3

Conserved Domains (3) summary

cd09358
Location:498 → 551

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam12130
Location:733 → 861

DUF3585; Protein of unknown function (DUF3585)

cd21196
Location:321 → 426

CH_MICAL1; calponin homology (CH) domain found in molecule interacting with CasL protein 1
XM_017313821.2 → XP_017169310.1 F-actin-monooxygenase MICAL1 isoform X5

Conserved Domains (2) summary

pfam00307
Location:670 → 713

CH; Calponin homology (CH) domain

pfam01494
Location:244 → 281

FAD_binding_3; FAD binding domain
XM_006512574.3 → XP_006512637.1 F-actin-monooxygenase MICAL1 isoform X4

Conserved Domains (3) summary

cd09358
Location:452 → 505

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam00307
Location:280 → 380

CH; Calponin homology (CH) domain

pfam12130
Location:687 → 815

DUF3585; Protein of unknown function (DUF3585)
XM_030244928.2 → XP_030100788.1 F-actin-monooxygenase MICAL1 isoform X3

Conserved Domains (3) summary

cd09358
Location:498 → 551

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam12130
Location:733 → 861

DUF3585; Protein of unknown function (DUF3585)

cd21196
Location:321 → 426

CH_MICAL1; calponin homology (CH) domain found in molecule interacting with CasL protein 1
XM_011243137.4 → XP_011241439.1 F-actin-monooxygenase MICAL1 isoform X2

See identical proteins and their annotated locations for XP_011241439.1

UniProtKB/Swiss-Prot

D3Z4C6, E9PXR1, Q3TB77, Q3TBH9, Q3TX89, Q8VDP3

UniProtKB/TrEMBL

E9PUI4

Conserved Domains (5) summary

cd09358
Location:683 → 736

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

COG2081
Location:88 → 224

YhiN; Predicted flavoprotein YhiN [General function prediction only]

pfam00307
Location:511 → 611

CH; Calponin homology (CH) domain

pfam01494
Location:85 → 122

FAD_binding_3; FAD binding domain

pfam12130
Location:918 → 1046

DUF3585; Protein of unknown function (DUF3585)
XM_036155655.1 → XP_036011548.1 F-actin-monooxygenase MICAL1 isoform X3

Conserved Domains (3) summary

cd09358
Location:498 → 551

LIM_Mical_like; The LIM domain of Mical (molecule interacting with CasL) like family

pfam12130
Location:733 → 861

DUF3585; Protein of unknown function (DUF3585)

cd21196
Location:321 → 426

CH_MICAL1; calponin homology (CH) domain found in molecule interacting with CasL protein 1