H1f6 H1.6 linker histone, cluster member [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: H1f6provided by RGD
Official Full Name: H1.6 linker histone, cluster memberprovided by RGD
Primary source: RGD:2778
See related: AllianceGenome:RGD:2778
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: H1D; H1t; H1-6; H1.3; H1f3; H1ft; H1s-4; Hist1h1t
Summary: Predicted to enable double-stranded DNA binding activity and nucleosomal DNA binding activity. Involved in spermatogenesis. Predicted to be located in condensed nuclear chromosome. Predicted to be part of nucleosome. Predicted to be active in nucleus. Orthologous to human H1-6 (H1.6 linker histone, cluster member). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: human mouse all
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Genomic context

Location:: 17p11

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (41855216..41855960, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (41427365..41428109, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (43675190..43675934, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

it appears that not the order of up-phosphorylation but the number of the phosphate groups is necessary for regulated chromatin decondensation, thus facilitating the substitution of H1t by transition proteins and protamines.
Title: Testis-specific linker histone H1t is multiply phosphorylated during spermatogenesis. Identification of phosphorylation sites.
RFX2 and NF-Y binding to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes
Title: Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.
studies suggest that Sp transcription factors may be involved in transcription of the histone H1t gene and the GC-box and the TE1 subelement are required for activation of the H1t promoter
Title: H1t/GC-box and H1t/TE1 element are essential for promoter activity of the testis-specific histone H1t gene.
The glutamine residue in histone H1t forms a hydrogen bond with main chain carbonyl of methionine-52 (in H1t) and is spatially oriented away from the nucleosome dyad axis.
Title: A K52Q substitution in the globular domain of histone H1t modulates its nucleosome binding properties.
RFX2 is strongly expressed in pachytene spermatocytes, remains high in early round spermatids, and declines only in advance of nuclear condensation. Maximum expression correlates well with the appearance of H1t.
Title: RFX2 is a potential transcriptional regulatory factor for histone H1t and other genes expressed during the meiotic phase of spermatogenesis.
These data support the hypothesis that RFX2 expression is upregulated in spermatocytes where it participates in activating transcription of the H1t gene and other testis genes.
Title: Transcription factor RFX2 is abundant in rat testis and enriched in nuclei of primary spermatocytes where it appears to be required for transcription of the testis-specific histone H1t gene.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D17Mgh3 (e-PCR)
- UniSTS:119316

UniSTS:491320 (e-PCR)
- UniSTS:491320

D17Wox14 (e-PCR)
- UniSTS:141928

D13Kyo1 (e-PCR), detects polymorphism
- UniSTS:141928

PMC123630P2 (e-PCR)
- UniSTS:270448

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables nucleosomal DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of chromatin	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within binding of sperm to zona pellucida	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in chromosome condensation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within flagellated sperm motility	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleosome assembly	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within spermatogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in condensed nuclear chromosome	ISO Inferred from Sequence Orthology more info
part_of nucleosome	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: histone H1t

Names: H1 histone family, member 3; H1 histone family, member T (testis-specific); histone 1 a family 3; histone 1, H1t; histone 1, family 3; histone 1t; histone cluster 1 H1 family member t; histone cluster 1, H1t; testis-specific histone 1, probably same as Hh1tts

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012579.1 → NP_036711.1 histone H1t

See identical proteins and their annotated locations for NP_036711.1

Status: PROVISIONAL

Source sequence(s)

M13170

UniProtKB/Swiss-Prot

P06349

UniProtKB/TrEMBL

A6KLN1

Conserved Domains (1) summary

cd00073
Location:36 → 116

H15; linker histone 1 and histone 5 domains; the basic subunit of chromatin is the nucleosome, consisting of an octamer of core histones, two full turns of DNA, a linker histone (H1 or H5) and a variable length of linker DNA; H1/H5 are chromatin-associated ...