Hc hemolytic complement [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hcprovided by MGI
Official Full Name: hemolytic complementprovided by MGI
Primary source: MGI:MGI:96031
See related: Ensembl:ENSMUSG00000026874 AllianceGenome:MGI:96031
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: C5; He; C5a; Hfib2
Summary: This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model. [provided by RefSeq, Nov 2015]
Expression: Biased expression in liver E18 (RPKM 32.3), liver adult (RPKM 24.7) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 23.22 cM

Exon count:: 43

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (34873341..34958518, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (34983329..35068506, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fcgr2b and Fcgr3 are the major genetic factors for cartilage antibody-induced arthritis, overriding the effect of Hc encoding complement C5.
Title: Fcgr2b and Fcgr3 are the major genetic factors for cartilage antibody-induced arthritis, overriding the effect of Hc encoding complement C5.
C5a-C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms.
Title: C5a-C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms.
C5a enhances inflammation and chemotaxis of gammadelta T cells in malignant pleural effusion.
Title: C5a enhances inflammation and chemotaxis of γδ T cells in malignant pleural effusion.
C5a-C5aR1 induces endoplasmic reticulum stress to accelerate vascular calcification via PERK-eIF2alpha-ATF4-CREB3L1 pathway.
Title: C5a-C5aR1 induces endoplasmic reticulum stress to accelerate vascular calcification via PERK-eIF2α-ATF4-CREB3L1 pathway.
C5a-licensed phagocytes drive sterilizing immunity during systemic fungal infection.
Title: C5a-licensed phagocytes drive sterilizing immunity during systemic fungal infection.
Effects of C5a and Receptor CD88 on Glutamate and N-Methyl-D-Aspartic Acid Receptor Expression in the Mouse Model of Optic Neuromyelitis.
Title: Effects of C5a and Receptor CD88 on Glutamate and N-Methyl-D-Aspartic Acid Receptor Expression in the Mouse Model of Optic Neuromyelitis.
Complement C5a promotes antigen cross-presentation by Peyer's patch monocyte-derived dendritic cells and drives a protective CD8(+) T cell response.
Title: Complement C5a promotes antigen cross-presentation by Peyer's patch monocyte-derived dendritic cells and drives a protective CD8(+) T cell response.
Complement C5 is not critical for the formation of sub-RPE deposits in Efemp1 mutant mice.
Title: Complement C5 is not critical for the formation of sub-RPE deposits in Efemp1 mutant mice.
Role of the C5a-C5a receptor axis in the inflammatory responses of the lungs after experimental polytrauma and hemorrhagic shock.
Title: Role of the C5a-C5a receptor axis in the inflammatory responses of the lungs after experimental polytrauma and hemorrhagic shock.
The C5a/C5aR2 axis promotes renal inflammation and tissue damage.
Title: The C5a/C5aR2 axis promotes renal inflammation and tissue damage.

Submit: New GeneRIF Correction See all GeneRIFs (123)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (10) 1 citation
Not Applicable (1) 1 citation
Spontaneous (1) 1 citation
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Hc (e-PCR), detects polymorphism
- UniSTS:257042

Hc (e-PCR), detects polymorphism
- UniSTS:143330

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables endopeptidase inhibitor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in complement activation, alternative pathway	IEA Inferred from Electronic Annotation more info
involved_in complement activation, classical pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glomerulus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in inflammatory response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within inflammatory response to wounding	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within kidney development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in killing of cells of another organism	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of macrophage chemotaxis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neutrophil homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chemokine production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular endothelial growth factor production	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in extracellular space	IEA Inferred from Electronic Annotation more info
part_of membrane attack complex	IEA Inferred from Electronic Annotation more info
part_of membrane attack complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: complement C5

Names: hepatic fibrogenesis 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010406.3 → NP_034536.3 complement C5 preproprotein

Status: VALIDATED

Source sequence(s)

AK080166, AL845534, M35525

Consensus CDS

CCDS15957.1

UniProtKB/Swiss-Prot

A2AS36, P06684

Related

ENSMUSP00000028233.4, ENSMUST00000028233.7

Conserved Domains (8) summary

COG2373
Location:64 → 614

YfaS; Uncharacterized conserved protein YfaS, alpha-2-macroglobulin family [General function prediction only]

cd00017
Location:686 → 754

ANATO; Anaphylatoxin homologous domain; C3a, C4a and C5a anaphylatoxins are protein fragments generated enzymatically in serum during activation of complement molecules C3, C4, and C5. They induce smooth muscle contraction. These fragments are homologous to ...

cd02896
Location:999 → 1304

complement_C3_C4_C5; Proteins similar to C3, C4 and C5 of vertebrate complement. The vertebrate complement system, comprised of a large number of distinct plasma proteins, is an effector of both the acquired and innate immune systems. The point of convergence of the ...

pfam01835
Location:125 → 219

A2M_N; MG2 domain

pfam00207
Location:776 → 863

A2M; Alpha-2-macroglobulin family

pfam07677
Location:1423 → 1513

A2M_recep; A-macroglobulin receptor

pfam07703
Location:470 → 610

A2M_N_2; Alpha-2-macroglobulin family N-terminal region

cl02512
Location:1531 → 1679

NTR_like; NTR_like domain; a beta barrel with an oligosaccharide/oligonucleotide-binding fold found in netrins, complement proteins, tissue inhibitors of metalloproteases (TIMP), and procollagen C-proteinase enhancers (PCOLCE), amongst others. In netrins, the ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

34873341..34958518 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017315669.2 → XP_017171158.1 complement C5 isoform X1

Conserved Domains (10) summary

cd00017
Location:690 → 758

ANATO; Anaphylatoxin homologous domain; C3a, C4a and C5a anaphylatoxins are protein fragments generated enzymatically in serum during activation of complement molecules C3, C4, and C5. They induce smooth muscle contraction. These fragments are homologous to ...

cd02896
Location:1003 → 1308

complement_C3_C4_C5; Proteins similar to C3, C4 and C5 of vertebrate complement. The vertebrate complement system, comprised of a large number of distinct plasma proteins, is an effector of both the acquired and innate immune systems. The point of convergence of the ...

pfam00207
Location:780 → 867

A2M; Alpha-2-macroglobulin family

pfam07677
Location:1427 → 1517

A2M_recep; A-macroglobulin receptor

pfam17789
Location:358 → 461

MG4; Macroglobulin domain MG4

pfam17790
Location:27 → 124

MG1; Macroglobulin domain MG1

pfam17791
Location:225 → 309

MG3; Macroglobulin domain MG3

pfam01835
Location:130 → 223

A2M_N; MG2 domain

pfam07703
Location:474 → 614

A2M_N_2; Alpha-2-macroglobulin family N-terminal region

cl02512
Location:1535 → 1683

NTR_like; NTR_like domain; a beta barrel with an oligosaccharide/oligonucleotide-binding fold found in netrins, complement proteins, tissue inhibitors of metalloproteases (TIMP), and procollagen C-proteinase enhancers (PCOLCE), amongst others. In netrins, the ...