GCN5 histone acetyltransferase GCN5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GCN5
Official Full Name: histone acetyltransferase GCN5
Primary source: SGD:S000003484
Locus tag: YGR252W
See related: AllianceGenome:SGD:S000003484; FungiDB:YGR252W; VEuPathDB:YGR252W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AAS104; ADA4; KAT2; SWI9
Summary: Enables N-acyltransferase activity; lysine-acetylated histone binding activity; and transcription coregulator activity. Involved in chromatin remodeling and positive regulation of transcription elongation by RNA polymerase II. Located in chromosome, centromeric region; cytosol; and nucleus. Part of ADA complex; SAGA complex; and SLIK (SAGA-like) complex. Orthologous to several human genes including KAT2A (lysine acetyltransferase 2A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GCN5 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (996869..998188)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Differential requirements for Gcn5 and NuA4 HAT activities in the starvation-induced versus basal transcriptomes.
Title: Differential requirements for Gcn5 and NuA4 HAT activities in the starvation-induced versus basal transcriptomes.
Acetylation of the influenza A virus polymerase subunit PA in the N-terminal domain positively regulates its endonuclease activity.
Title: Acetylation of the influenza A virus polymerase subunit PA in the N-terminal domain positively regulates its endonuclease activity.
Gcn5p and Ubp8p Affect Protein Ubiquitylation and Cell Proliferation by Altering the Fermentative/Respiratory Flux Balance in Saccharomyces cerevisiae.
Title: Gcn5p and Ubp8p Affect Protein Ubiquitylation and Cell Proliferation by Altering the Fermentative/Respiratory Flux Balance in Saccharomyces cerevisiae.
Gcn5 and Rpd3 have a limited role in the regulation of cell cycle transcripts during the G1 and S phases in Saccharomyces cerevisiae.
Title: Gcn5 and Rpd3 have a limited role in the regulation of cell cycle transcripts during the G1 and S phases in Saccharomyces cerevisiae.
The Gcn5-Ada2-Ada3 (ADA) histone acetyltransferase complexes have the capacity to crotonylate histones and crotonylate lysines in the N-terminal tails of histone H3 and H4. Crotonylation selectively affects gene transcription in vivo in a manner dependent on Gcn5 and Esa1.
Title: Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription.
work argues that nonhistone protein acetylation by Gcn5 is determined in part by specific amino acids surrounding target lysines but that even optimal sequences require both Ada2 and Ada3 for robust acetylation.
Title: A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs.
the Ada2 SANT domain does not activate Gcn5's activity by directly affecting histone peptide binding as previously proposed. Instead, the Ada2 SANT domain enhances Gcn5 binding of the enzymatic cosubstrate acetyl-CoA.
Title: Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
This replication-guided H3K9ac was fully dependent on the acetyltransferase Rtt109, while expression-guided H3K9ac was deposited by Gcn5. Further, topoisomerase depletion intensified H3K9ac in front of the replication fork and in sites where RNA polymerase II was trapped, suggesting supercoiling stresses trigger H3K9 acetylation.
Title: Chromatin dynamics during DNA replication.
It conserved Spt-Ada-Gcn5 acetyltransferase (SAGA) complex as a paradigm to illustrate how co-activators share and combine a relatively limited set of functional tools.
Title: Sharing the SAGA.
Gcn5 acetyltransferase synergizes with Spt3 (TBP-binding) to promote transcription.
Title: SAGA Is a General Cofactor for RNA Polymerase II Transcription.

Submit: New GeneRIF Correction See all GeneRIFs (50)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AK5 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AK9 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BK12 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BK5 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3 acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3 acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3 acetyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables histone H3 acetyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K122 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K14 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K18 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K23 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K27 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K36 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K4 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K56 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K9 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H4K12 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H4K16 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H4K5 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H4K8 acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone crotonyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone crotonyltransferase activity	IEA Inferred from Electronic Annotation more info
enables lysine-acetylated histone binding	IDA Inferred from Direct Assay more info	PubMed
enables peptide-lysine-N-acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables transcription coregulator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription elongation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of ADA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SLIK (SAGA-like) complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of histone acetyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: histone acetyltransferase GCN5

NP_011768.1

EC 2.3.1.48

Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

996869..998188

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181381.1 → NP_011768.1 TPA: histone acetyltransferase GCN5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011768.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV32, Q03330, Q6B165

UniProtKB/TrEMBL

B3LHY8, B5VJJ4, C7GNH8, C8Z9E4, G2WEX8, N1P4F7

Conserved Domains (1) summary

COG5076
Location:77 → 439

COG5076; Transcription factor involved in chromatin remodeling, contains bromodomain [Chromatin structure and dynamics / Transcription]