spn-E spindle E [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: spn-Eprovided by FlyBase
Official Full Name: spindle Eprovided by FlyBase
Primary source: FLYBASE:FBgn0003483
Locus tag: Dmel_CG3158
See related: AllianceGenome:FB:FBgn0003483
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3158; Dmel\CG3158; fs(1)hls; fs(3)hls; hes; hls; homeless; spin-E; Spindle-E; spn E; Spn-E; SPN-E; spn-E/hls; spnE; SpnE
Summary: Predicted to enable RNA binding activity and helicase activity. Involved in several processes, including negative regulation of gene expression; oogenesis; and specification of axis polarity. Located in P granule and nucleus. Is expressed in egg chamber; female germline stem cell; germarium; nurse cell; and ovary. Human ortholog(s) of this gene implicated in spermatogenic failure 30. Orthologous to human TDRD9 (tudor domain containing 9). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 89A5-89A5; 3-58 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (15837419..15844415)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (11663141..11670137)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.
DExH box domain of Spindle-E is necessary for its function in the piRNA pathway and retrotransposon silencing during oogenesis and embryonic patterning.
Title: The DExH box helicase domain of spindle-E is necessary for retrotransposon silencing and axial patterning during Drosophila oogenesis.
RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.
Title: RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.
mutations in piwi, aubergine, or spindle-E which encode RNAi components result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2
Title: Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on RNA	TAS Traceable Author Statement more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	TAS Traceable Author Statement more info	PubMed
enables helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA localization	TAS Traceable Author Statement more info	PubMed
involved_in dorsal appendage formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in global gene silencing by mRNA cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	TAS Traceable Author Statement more info	PubMed
involved_in mitotic chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte karyosome formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in piRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polarity specification of anterior/posterior axis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polarity specification of dorsal/ventral axis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole plasm assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in secondary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by piRNA-mediated mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	NAS Non-traceable Author Statement more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: spindle E

Names: CG3158-PA; Spindle-E (homeless); SpindleE; female sterile(3)homeless; homeless; homeless/spindle-E; spindle-E; spn-E-PA

NP_476741.1

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

15837419..15844415

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057393.4 → NP_476741.1 spindle E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476741.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A2RVD0, Q26453, Q9VF26

UniProtKB/TrEMBL

L0CPW3, L0CR04, L0CRC8

Related

FBpp0082637

Conserved Domains (2) summary

COG1643
Location:113 → 697

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]

pfam00567
Location:892 → 1016

TUDOR; Tudor domain