nec necrotic [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: necprovided by FlyBase
Official Full Name: necroticprovided by FlyBase
Primary source: FLYBASE:FBgn0002930
Locus tag: Dmel_CG1857
See related: AllianceGenome:FB:FBgn0002930
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0118057.3; CG1857; Dmel\CG1857; Nec; NEC; Ser3; SER3; Spn43Ac
Summary: Enables serine-type endopeptidase inhibitor activity. Involved in negative regulation of Toll receptor ligand protein activation cascade; negative regulation of innate immune response; and negative regulation of proteolysis. Located in extracellular space. Is expressed in adult head; embryonic/larval fat body; embryonic/larval hindgut; embryonic/larval midgut; and organism. Used to study alpha 1-antitrypsin deficiency. Human ortholog(s) of this gene implicated in several diseases, including intermediate coronary syndrome; neurodegenerative disease (multiple); thrombophilia (multiple); toxic shock syndrome; and type 2 diabetes mellitus. Orthologous to several human genes including SERPINA12 (serpin family A member 12); SERPINB1 (serpin family B member 1); and SERPINC1 (serpin family C member 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 42F3-42F3; 2-55 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (7156477..7158241, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (3043982..3045746, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data demonstrate a critical role for basic residues within the D-helix (and lysine 68 in particular) in the inhibitory mechanism of the serpin Necrotic.
Title: Inhibitory activity of the Drosophila melanogaster serpin Necrotic is dependent on lysine residues in the D-helix.
finding indicates that cleavage of the Necrotic amino-terminal extension might modulate Toll activation following the initial immune response
Title: Immune challenge induces N-terminal cleavage of the Drosophila serpin Necrotic.
likely to inhibit a wide range of proteases in Drosophila and has the specificity requirements to act as the physiological inhibitor of Persephone in vivo
Title: Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables serine-type endopeptidase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase inhibitor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in negative regulation of Toll receptor ligand protein activation cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of Toll receptor ligand protein activation cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to fungus	HEP more info	PubMed

Component	Evidence Code	Pubs
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: necrotic

Names: CG1857-PA; nec-PA; necrotic serpin; serine protease inhibitor 3; serpin43Ac

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

7156477..7158241 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080112.2 → NP_524851.1 necrotic [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524851.1

Status: REVIEWED

UniProtKB/TrEMBL

Q7JWX3, Q9U5W7

Related

FBpp0088123

Conserved Domains (1) summary

cd00172
Location:108 → 468

SERPIN; SERine Proteinase INhibitors (serpins) exhibit conformational polymorphism shifting from native to cleaved, latent, delta, or polymorphic forms. Many serpins, such as antitrypsin and antichymotrypsin, function as serine protease inhibitors which regulate ...

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q6V6U1		Q6V6U1
Q6V6U2		Q6V6U2
Q6V6U3		Q6V6U3
Q6V6U4		Q6V6U4
Q6V6U5		Q6V6U5
Q6V6U6		Q6V6U6
Q6V6U7		Q6V6U7
Q6V6U8		Q6V6U8
Q6V6U9		Q6V6U9
Q6V6V0		Q6V6V0
Q7JWX3		Q7JWX3
Q9U5W7		Q9U5W7