alg-1 Protein argonaute-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: alg-1
Official Full Name: Protein argonaute-2
Primary source: WormBase:WBGene00000105
Locus tag: CELE_F48F7.1
See related: AllianceGenome:WB:WBGene00000105
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables RNA binding activity and RNA endonuclease activity. Involved in several processes, including regulation of development, heterochronic; regulatory ncRNA-mediated gene silencing; and vulval development. Located in P-body. Part of RISC complex. Is expressed in several structures, including hypodermis; nervous system; pharynx; reproductive system; and somatic cell. Human ortholog(s) of this gene implicated in alcohol dependence and glaucoma. Orthologous to several human genes including AGO2 (argonaute RISC catalytic component 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See alg-1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 7

Sequence:: Chromosome: X; NC_003284.9 (13949489..13956261)

Chromosome X - NC_003284.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003284.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals.
Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals.
An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress.
Title: An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress.
The alg-1 Gene Is Necessary for Orsay Virus Replication in Caenorhabditis elegans.
Title: The alg-1 Gene Is Necessary for Orsay Virus Replication in Caenorhabditis elegans.
The RabGAP TBC-11 controls Argonaute localization for proper microRNA function in C. elegans.
Title: The RabGAP TBC-11 controls Argonaute localization for proper microRNA function in C. elegans.
Alg-1 regulates mRNA splicing pattern in muscle and intestine by modulating splicing factor activities.
Title: ALG-1 Influences Accurate mRNA Splicing Patterns in the Caenorhabditis elegans Intestine and Body Muscle Tissues by Modulating Splicing Factor Activities.
knockdown of alg-1 and alg-2 in germ cells results in few defects in oocyte maturation and ovulation.
Title: Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans.
Pairing beyond the seed supports ALG-1 microRNA targeting specificity.
Title: Pairing beyond the Seed Supports MicroRNA Targeting Specificity.
Altogether, these data suggest that the phosphorylation state of the serine/threonine cluster is important for Argonaute-mRNA interactions.
Title: Phosphorylation of Argonaute proteins affects mRNA binding and is essential for microRNA-guided gene silencing in vivo.
We show in vitro and in vivo that their mutations severely altered the association with AIN-1 and AIN-2. our results demonstrate that the microRNA pathway can act independently of GW182 proteins during C. elegans embryogenesis
Title: GW182-Free microRNA Silencing Complex Controls Post-transcriptional Gene Expression during Caenorhabditis elegans Embryogenesis.
ALG-1 complexes recognize structural features of particular microRNAs in the context of conducting the strand selection and microRNA* ejection steps of miRISC maturation
Title: Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F48F7.1 (e-PCR)
- UniSTS:321486

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity, producing 3'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables endoribonuclease activity, cleaving siRNA-paired mRNA	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of development, heterochronic	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in vulval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Protein argonaute-2

NP_001367206.1

Confirmed by transcript evidence

NP_001379113.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003284.9 Reference assembly

Range

13949489..13956261

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001381140.1 → NP_001367206.1 Protein argonaute-2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

G5EES3, Q3LTR7

Conserved Domains (4) summary

cd02846
Location:390 → 511

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:556 → 981

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:310 → 389

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:170 → 300

ArgoN; N-terminal domain of argonaute
NM_001392882.1 → NP_001379113.1 Protein argonaute-2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

G5EGR6, Q3LTR7

Conserved Domains (4) summary

cd02846
Location:369 → 490

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:535 → 960

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:289 → 368

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:149 → 279

ArgoN; N-terminal domain of argonaute