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    Gclc glutamate-cysteine ligase, catalytic subunit [ Mus musculus (house mouse) ]

    Gene ID: 14629, updated on 4-Jan-2025

    Summary

    Official Symbol
    Gclcprovided by MGI
    Official Full Name
    glutamate-cysteine ligase, catalytic subunitprovided by MGI
    Primary source
    MGI:MGI:104990
    See related
    Ensembl:ENSMUSG00000032350 AllianceGenome:MGI:104990
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Glclc; GLCL-H; Ggcs-hs; D9Wsu168e
    Summary
    Enables glutamate-cysteine ligase activity. Acts upstream of or within several processes, including glutathione biosynthetic process; negative regulation of apoptotic signaling pathway; and regulation of protein metabolic process. Located in cytosol. Part of glutamate-cysteine ligase complex. Is expressed in several structures, including alimentary system; central nervous system; integumental system; metanephros; and sensory organ. Used to study cataract. Human ortholog(s) of this gene implicated in cystic fibrosis; glutamate-cysteine ligase deficiency; hemolytic anemia; and myocardial infarction. Orthologous to human GCLC (glutamate-cysteine ligase catalytic subunit). [provided by Alliance of Genome Resources, Jan 2025]
    Expression
    Ubiquitous expression in liver adult (RPKM 50.5), kidney adult (RPKM 34.6) and 28 other tissues See more
    Orthologs
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    Genomic context

    See Gclc in Genome Data Viewer
    Location:
    9 E1; 9 43.36 cM
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (77661817..77701771)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (77754535..77794489)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_24700 Neighboring gene chimerin (chimaerin) 1 pseudogene Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:77440338-77440521 Neighboring gene STARR-positive B cell enhancer ABC_E2905 Neighboring gene STARR-seq mESC enhancer starr_24701 Neighboring gene STARR-seq mESC enhancer starr_24702 Neighboring gene STARR-positive B cell enhancer ABC_E6770 Neighboring gene kelch-like 31 Neighboring gene STARR-seq mESC enhancer starr_24703 Neighboring gene STARR-seq mESC enhancer starr_24704 Neighboring gene STARR-positive B cell enhancer ABC_E3846 Neighboring gene STARR-seq mESC enhancer starr_24706 Neighboring gene predicted gene, 34654 Neighboring gene STARR-positive B cell enhancer mm9_chr9:77758381-77758682 Neighboring gene STARR-positive B cell enhancer ABC_E6772 Neighboring gene STARR-positive B cell enhancer ABC_E2906 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:77766361-77766544 Neighboring gene STARR-positive B cell enhancer mm9_chr9:77778672-77778973 Neighboring gene ELOVL fatty acid elongase 5 Neighboring gene predicted gene, 34771

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (5)  1 citation

    Pathways from PubChem

    Interactions

    General gene information

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ADP binding  
    enables ADP binding  
    enables ATP binding  
    enables glutamate binding  
    enables glutamate binding  
    enables glutamate-cysteine ligase activity  
    enables glutamate-cysteine ligase activity PubMed 
    enables glutamate-cysteine ligase activity PubMed 
    enables glutamate-cysteine ligase activity  
    enables glutamate-cysteine ligase activity  
    enables magnesium ion binding  
    enables magnesium ion binding  
    enables protein binding PubMed 
    enables protein binding  
    enables protein-containing complex binding  
    enables protein-containing complex binding  
    Items 1 - 25 of 51
    Process Evidence Code Pubs
    acts_upstream_of_or_within L-ascorbic acid metabolic process PubMed 
    involved_in blood vessel diameter maintenance  
    involved_in blood vessel diameter maintenance  
    involved_in cell redox homeostasis  
    involved_in cell redox homeostasis  
    involved_in cellular response to fibroblast growth factor stimulus  
    involved_in cellular response to follicle-stimulating hormone stimulus  
    involved_in cellular response to glucose stimulus  
    involved_in cellular response to hepatocyte growth factor stimulus  
    involved_in cellular response to insulin stimulus  
    involved_in cellular response to mechanical stimulus  
    involved_in cellular response to thyroxine stimulus  
    involved_in cysteine metabolic process  
    involved_in cysteine metabolic process  
    involved_in glutamate metabolic process  
    involved_in glutamate metabolic process  
    involved_in glutathione biosynthetic process  
    acts_upstream_of_or_within glutathione biosynthetic process PubMed 
    involved_in glutathione biosynthetic process  
    acts_upstream_of_or_within glutathione biosynthetic process PubMed 
    involved_in glutathione biosynthetic process  
    involved_in glutathione biosynthetic process  
    acts_upstream_of_or_within glutathione metabolic process PubMed 
    involved_in negative regulation of DNA-templated transcription  
    involved_in negative regulation of DNA-templated transcription  
    involved_in negative regulation of apoptotic process  
    involved_in negative regulation of apoptotic process  
    acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway PubMed 
    involved_in negative regulation of hepatic stellate cell activation  
    involved_in negative regulation of hepatic stellate cell activation  
    acts_upstream_of_or_within negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway PubMed 
    involved_in negative regulation of neuron apoptotic process  
    involved_in negative regulation of neuron apoptotic process  
    acts_upstream_of_or_within negative regulation of protein ubiquitination PubMed 
    acts_upstream_of_or_within positive regulation of proteasomal ubiquitin-dependent protein catabolic process PubMed 
    acts_upstream_of_or_within regulation of mitochondrial depolarization PubMed 
    involved_in response to activity  
    acts_upstream_of_or_within response to arsenic-containing substance PubMed 
    involved_in response to cadmium ion  
    involved_in response to heat  
    involved_in response to heat  
    involved_in response to hormone  
    involved_in response to hormone  
    involved_in response to human chorionic gonadotropin  
    involved_in response to interleukin-1  
    involved_in response to nitrosative stress  
    involved_in response to nitrosative stress  
    involved_in response to nutrient  
    involved_in response to oxidative stress  
    involved_in response to oxidative stress  
    acts_upstream_of_or_within response to xenobiotic stimulus PubMed 
    Items 1 - 25 of 51
    Component Evidence Code Pubs
    located_in cytosol PubMed 
    part_of glutamate-cysteine ligase complex  
    part_of glutamate-cysteine ligase complex PubMed 
    part_of glutamate-cysteine ligase complex  
    located_in mitochondrion  

    General protein information

    Preferred Names
    glutamate--cysteine ligase catalytic subunit
    Names
    GCS heavy chain
    gamma GCS-HS
    gamma-ECS
    gamma-glutamylcysteine synthetase heavy subunit
    NP_034425.1
    XP_006510875.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_010295.2NP_034425.1  glutamate--cysteine ligase catalytic subunit

      See identical proteins and their annotated locations for NP_034425.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the protein-coding transcript.
      Source sequence(s)
      AK144339, AK160876, EH103060
      Consensus CDS
      CCDS23354.1
      UniProtKB/Swiss-Prot
      O09166, P97494, Q8VCS5
      UniProtKB/TrEMBL
      Q3TEF1, Q3UNA7
      Related
      ENSMUSP00000034905.9, ENSMUST00000034905.9
      Conserved Domains (1) summary
      pfam03074
      Location:236608
      GCS; Glutamate-cysteine ligase

    RNA

    1. NR_151778.1 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AC159886, AC160124

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      77661817..77701771
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006510812.2XP_006510875.1  glutamate--cysteine ligase catalytic subunit isoform X1

      UniProtKB/TrEMBL
      Q3UNA7
      Conserved Domains (1) summary
      pfam03074
      Location:183555
      GCS; Glutamate-cysteine ligase
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