Orai orai [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Oraiprovided by FlyBase
Official Full Name: oraiprovided by FlyBase
Primary source: FLYBASE:FBgn0041585
Locus tag: Dmel_CG11430
See related: AllianceGenome:FB:FBgn0041585
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA:LD03241; CG11430; CRACM1; Dmel\CG11430; dorai; dOrai; DOrai; dOrai1; l(2)k11505; olf186-F; Olf186-F; orai; ORAI; Orai1; ORAI1
Summary: Enables store-operated calcium channel activity. Involved in calcium ion transport; positive regulation of calcineurin-NFAT signaling cascade; and positive regulation of calcium ion transport. Part of calcium channel complex. Is expressed in adult head; organism; wing hinge primordium; and wing pouch. Used to study dilated cardiomyopathy and hypertrophic cardiomyopathy. Human ortholog(s) of this gene implicated in immunodeficiency 9 and tubular aggregate myopathy 2. Orthologous to several human genes including ORAI2 (ORAI calcium release-activated calcium modulator 2) and ORAI3 (ORAI calcium release-activated calcium modulator 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 54F1-54F3; 2-84 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17843442..17860582)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (13730947..13748087)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore.
Title: Structures reveal opening of the store-operated calcium channel Orai.
Ca2+ uptake in neurons with reduced SEPT7 requires Orai.
Title: Store-independent modulation of Ca(2+) entry through Orai by Septin 7.
after depletion of Ca(2+) stores in Drosophila, translocation of the IP3R to Endoplasmic reticulum-plasma-membrane junctions facilitates the coupling of STIM to Orai that leads to activation of Store-operated Ca(2+) entry.
Title: Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM-Orai interactions in Drosophila neurons.
These studies suggest that modulation of SOCE could serve as a novel mechanism for restoring dopamine levels in dopaminergic neurons.
Title: Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila.
Data indicate that STIM functions as sensors of Ca2+ stored in the endoplasmic reticulum (ER) lumen and translocate into ER-PM (plasma membrane) junctions to tether and activate Orai channels when ER Ca2+ concentration decreases.
Title: Orai channel pore properties and gating by STIM: implications from the Orai crystal structure.
The crystal structure of Orai from Drosophila melanogaster, determined at 3.35 angstrom resolution, reveals that the calcium channel is composed of a hexameric assembly of Orai subunits arranged around a central ion pore.
Title: Crystal structure of the calcium release-activated calcium channel Orai.
Interaction with the C terminus of Stim thus induces Orai dimers to dimerize, forming tetramers that constitute the Ca(2+)-selective pore.
Title: The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers.
results demonstrate that the protein CRACM1 encoded by olf186-F is essential for store-operated Ca2+ influx via Ca2+ release-activated Ca2+ (CRAC) channels
Title: CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.
results indicate that Orai itself forms the Ca2+-selectivity filter of the CRAC channel
Title: Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai.
Orai1 is an essential component or regulator of the CRAC channel complex
Title: A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calcium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium channel regulator activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables store-operated calcium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables store-operated calcium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables store-operated calcium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium ion transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of calcineurin-NFAT signaling cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of calcium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in store-operated calcium entry	IBA Inferred from Biological aspect of Ancestor more info
involved_in store-operated calcium entry	IDA Inferred from Direct Assay more info	PubMed
involved_in store-operated calcium entry	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of calcium channel complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: orai

Names: CG11430-PA; CG11430-PB; CG11430-PE; CG11430-PF; CG11430-PG; CRAC modulator 1; Orai-PA; Orai-PB; Orai-PE; Orai-PF; Orai-PG; lethal (2) k11505; olf186-F