Neto1 neuropilin (NRP) and tolloid (TLL)-like 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Neto1provided by MGI
Official Full Name: neuropilin (NRP) and tolloid (TLL)-like 1provided by MGI
Primary source: MGI:MGI:2180216
See related: Ensembl:ENSMUSG00000050321 AllianceGenome:MGI:2180216
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Btcl1; C130005O10Rik
Summary: Enables ionotropic glutamate receptor binding activity. Involved in learning or memory; modulation of chemical synaptic transmission; and protein localization to synapse. Acts upstream of or within positive regulation of excitatory postsynaptic potential and receptor localization to synapse. Located in excitatory synapse and postsynaptic density. Is active in Schaffer collateral - CA1 synapse; glutamatergic synapse; and postsynaptic density membrane. Is expressed in central nervous system; metencephalon lateral wall; and retina inner nuclear layer. Orthologous to human NETO1 (neuropilin and tolloid like 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in frontal lobe adult (RPKM 3.4), cortex adult (RPKM 2.9) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 E4; 18 58.47 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (86411110..86524482)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (86394815..86506721)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Aging to 24 months increased C57BL/6J mouse social sniffing and hippocampal Neto1 levels, and impaired female spatial learning.
Title: Aging to 24 months increased C57BL/6J mouse social sniffing and hippocampal Neto1 levels, and impaired female spatial learning.
Neto1 and Neto2 differentially regulate GluK2 fast gating kinetics
Title: Neto proteins regulate gating of the kainate-type glutamate receptor GluK2 through two binding sites.
NETO1 is required for formation of Kainate type ionotropic glutamate receptors-containing synapses in interneurons.
Title: NETO1 Regulates Postsynaptic Kainate Receptors in CA3 Interneurons During Circuit Maturation.
The data of this study showed that a novel role for NETO1 in regulation of axonal Kainate Receptors and identify its physiological significance in development of the CA3-CA1 circuit.
Title: NETO1 Guides Development of Glutamatergic Connectivity in the Hippocampus by Regulating Axonal Kainate Receptors.
KAR-mediated excitation of interneurons is contingent upon Neto1 because kainate yields comparable effects in Neto2 knockouts and wild-types but fails to excite interneurons or recruit inhibition in Neto1 knockouts.
Title: Neto Auxiliary Subunits Regulate Interneuron Somatodendritic and Presynaptic Kainate Receptors to Control Network Inhibition.
GluK2 surface and synaptic expression are independent on the auxiliary subunits Neto1 and Neto2. In the CA1 hippocampal pyramidal cell, GluK2, in contrast to GluK1, traffics to the neuronal surface and synapse efficiently and the auxiliary subunits rNeto1 and mNeto2 caused no further enhancement of these 2 trafficking processes.
Title: Amino-terminal domains of kainate receptors determine the differential dependence on Neto auxiliary subunits for trafficking.
These results establish critical roles for Neto auxiliary subunits controlling Kainate receptor properties and synaptic incorporation.
Title: Neto auxiliary proteins control both the trafficking and biophysical properties of the kainate receptor GluK1.
These results confirm that Neto protein interactions regulate synaptic localization of NMDA and kainate receptor subunits at mossy fiber-CA3 pyramidal cell synapses.
Title: Neto auxiliary protein interactions regulate kainate and NMDA receptor subunit localization at mossy fiber-CA3 pyramidal cell synapses.
This study demonistrated that Neto1 to regulate both NMDARs and KARs reveals a unique dual role in controlling synaptic transmission by serving as an auxiliary protein for these two classes of ionotropic glutamate receptors in a synapse-specific fashion.
Title: Neto1 is an auxiliary subunit of native synaptic kainate receptors.
The results of this study indicated that two distinctive properties of native kainate receptors, their high-affinity binding pattern in the brain and slow kinetics at postsynapses, are conferred by the kainate receptors auxiliary subunit Neto1.
Title: Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ionotropic glutamate receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in anterograde axonal transport of neurotransmitter receptor complex	IDA Inferred from Direct Assay more info	PubMed
involved_in anterograde axonal transport of neurotransmitter receptor complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter receptor localization to postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter receptor localization to postsynaptic specialization membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of excitatory postsynaptic potential	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within receptor localization to synapse	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of kainate selective glutamate receptor activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of long-term neuronal synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in visual learning	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in axon cytoplasm	IEA Inferred from Electronic Annotation more info
located_in excitatory synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density membrane	EXP Inferred from Experiment more info	PubMed
is_active_in postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density membrane	IEP Inferred from Expression Pattern more info	PubMed
is_active_in postsynaptic density membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neuropilin and tolloid-like protein 1

Names: brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001360910.1 → NP_001347839.1 neuropilin and tolloid-like protein 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks alternate in-frame exons in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AC113599

Conserved Domains (2) summary

cl00049
Location:16 → 90

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:95 → 130

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
NM_144946.5 → NP_659195.3 neuropilin and tolloid-like protein 1 isoform 1 precursor

See identical proteins and their annotated locations for NP_659195.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC113599

Consensus CDS

CCDS29387.1

UniProtKB/Swiss-Prot

Q80X39, Q8C4S3, Q8CCM2, Q8R4I7

Related

ENSMUSP00000057340.3, ENSMUST00000058829.4

Conserved Domains (2) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:291 → 326

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

86411110..86524482

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006526481.5 → XP_006526544.1 neuropilin and tolloid-like protein 1 isoform X1

See identical proteins and their annotated locations for XP_006526544.1

UniProtKB/Swiss-Prot

Q80X39, Q8C4S3, Q8CCM2, Q8R4I7

Conserved Domains (2) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:291 → 326

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_006526480.5 → XP_006526543.1 neuropilin and tolloid-like protein 1 isoform X1

See identical proteins and their annotated locations for XP_006526543.1

UniProtKB/Swiss-Prot

Q80X39, Q8C4S3, Q8CCM2, Q8R4I7

Conserved Domains (2) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:291 → 326

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_036161154.1 → XP_036017047.1 neuropilin and tolloid-like protein 1 isoform X3

Conserved Domains (2) summary

cl00049
Location:16 → 90

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:95 → 130

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_036161152.1 → XP_036017045.1 neuropilin and tolloid-like protein 1 isoform X1

UniProtKB/Swiss-Prot

Q80X39, Q8C4S3, Q8CCM2, Q8R4I7

Conserved Domains (2) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:291 → 326

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_036161151.1 → XP_036017044.1 neuropilin and tolloid-like protein 1 isoform X1

UniProtKB/Swiss-Prot

Q80X39, Q8C4S3, Q8CCM2, Q8R4I7

Conserved Domains (2) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:291 → 326

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...
XM_017317909.3 → XP_017173398.1 neuropilin and tolloid-like protein 1 isoform X4

Conserved Domains (1) summary

cd00041
Location:51 → 154

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.
XM_011247081.4 → XP_011245383.1 neuropilin and tolloid-like protein 1 isoform X5

Conserved Domains (1) summary

pfam00431
Location:51 → 152

CUB; CUB domain
XM_036161153.1 → XP_036017046.1 neuropilin and tolloid-like protein 1 isoform X2

Conserved Domains (2) summary

cd00041
Location:25 → 111

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cl00104
Location:116 → 151

LDLa; Low Density Lipoprotein Receptor Class A domain, a cysteine-rich repeat that plays a central role in mammalian cholesterol metabolism; the receptor protein binds LDL and transports it into cells by endocytosis; 7 successive cysteine-rich repeats of about ...