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    UBC35 ubiquitin-conjugating enzyme 35 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 844224, updated on 18-Sep-2024

    Summary

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    Official Symbol
    UBC35
    Official Full Name
    ubiquitin-conjugating enzyme 35
    Primary source
    TAIR:AT1G78870
    Locus tag
    AT1G78870
    See related
    Araport:AT1G78870
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
    Summary
    UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.
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    Genomic context

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    See UBC35 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    7
    Sequence:
    Chromosome: 1; NC_003070.9 (29650376..29652627)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene D-mannose binding lectin protein with Apple-like carbohydrate-binding domain-containing protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene Ubiquitin-specific protease family C19-related protein Neighboring gene uncharacterized protein Neighboring gene Reticulon family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Arabidopsis UBC13 differentially regulates two programmed cell death pathways in responses to pathogen and low-temperature stress. Wang L, et al. New Phytol, 2019 Jan. PMID 30218535
    2. UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability. Wen R, et al. Plant J, 2014 Nov. PMID 25142088
    3. A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots. Li W, et al. Plant J, 2010 Apr. PMID 20113438
    4. Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis. Yin XJ, et al. Plant Cell, 2007 Jun. PMID 17586653, Free PMC Article
    5. Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants. Wen R, et al. Plant Mol Biol, 2006 May. PMID 16786304

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The results revealed that UBC13 is a key regulator in plant response to low temperature and pathogens.
      Title: Arabidopsis UBC13 differentially regulates two programmed cell death pathways in responses to pathogen and low-temperature stress.
    2. UBC13 activity is critical for all major aspects of root development. The double mutant plants were insensitive to auxin treatments and the ubc13 mutant had a reduced auxin response.
      Title: UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability.
    3. Arabidopsis contains two highly conserved and likely duplicated UBC13 genes, AtUBC13A and AtUBC13B. Data suggests the existence of an error-free DNA damage tolerance pathway in plants. [UBC13A]
      Title: Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001036221.3
    NM_001334850.1
    NM_001334851.1
    NM_106535.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin conjugating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within postreplication repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to iron ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within root epidermal cell differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of UBC13-MMS2 complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in mitochondrion HDA PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin-conjugating enzyme 35
    NP_001031298.1
    • ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_001319409.1
    • ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 10164 Blast hits to 10157 proteins in 398 species: Archae - 0; Bacteria - 2; Metazoa - 4429; Fungi - 2211; Plants - 1825; Viruses - 23; Other Eukaryotes - 1674 (source: NCBI BLink).
    NP_565192.1
    • ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      29650376..29652627
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_106535.4 → NP_565192.1  ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_565192.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q2V4C1, Q94A97, Q9ZVA6
      UniProtKB/TrEMBL
      A0A178WFI9, A0A5S9WVM9
      Conserved Domains (1) summary
      cl00154
      Location:6 → 150
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

    2. NM_001334851.1 → NP_001321618.1  ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1P8ARI0

    3. NM_001334850.1 → NP_001319409.1  ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A2H1ZEG2

    4. NM_001036221.3 → NP_001031298.1  ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001031298.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q94A97
      Conserved Domains (2) summary
      COG5078
      Location:1 → 108
      COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
      cl00154
      Location:6 → 108
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q94A97.1

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