Chrm3 cholinergic receptor, muscarinic 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chrm3provided by RGD
Official Full Name: cholinergic receptor, muscarinic 3provided by RGD
Primary source: RGD:2343
See related: EnsemblRapid:ENSRNOG00000049410 AllianceGenome:RGD:2343
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including G protein-coupled acetylcholine receptor activity; G protein-coupled acetylcholine receptor binding activity; and acetylcholine binding activity. Involved in G protein-coupled acetylcholine receptor signaling pathway; negative regulation of heart rate by acetylcholine; and positive regulation of vascular associated smooth muscle contraction. Located in several cellular components, including asymmetric synapse; axon terminus; and dendrite. Is active in glutamatergic synapse; postsynaptic density membrane; and presynaptic membrane. Human ortholog(s) of this gene implicated in bladder disease; chronic obstructive pulmonary disease; prune belly syndrome; and pulmonary fibrosis. Orthologous to human CHRM3 (cholinergic receptor muscarinic 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 55.0), Kidney (RPKM 22.4) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 17q12.1

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (64696549..65158622)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (60005137..60467250)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (63990599..64463222, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Establishment of a CaCC-based Cell Model and Method for High-throughput Screening of M3 Receptor Drugs.
Title: Establishment of a CaCC-based Cell Model and Method for High-throughput Screening of M3 Receptor Drugs.
M3 but not M4 muscarinic receptors in the rostromedial tegmental nucleus are involved in the acquisition of morphine-induced conditioned place preference.
Title: M(3) but not M(4) muscarinic receptors in the rostromedial tegmental nucleus are involved in the acquisition of morphine-induced conditioned place preference.
Eliminating muscarinic cholinergic 3 receptor (M3R) from Dahl salt sensitive (DSS) rats Improves diastolic dysfunction.
Title: Novel Pathogenesis of Hypertension and Diastolic Dysfunction Caused by M3R (Muscarinic Cholinergic 3 Receptor) Signaling.
Data suggest that, in atrial myocardium (or isolated cardiomyocytes), activation of muscarinic receptors M3 (Chrm3) leads to shortening of action potentials via suppression of L-type calcium channels; the major potassium channels are not affected, but inwardly rectifying potassium channels are suppressed.
Title: M3 cholinoreceptors alter electrical activity of rat left atrium via suppression of L-type Ca(2+) current without affecting K(+) conductance.
M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.
Title: M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.
Our results suggest that the mechanism for the deficits in learning and memory of rats with chronic fluorosis may be associated with the decreased expressions of M1 and M3 in mAChRs
Title: Deficit in learning and memory of rats with chronic fluorosis correlates with the decreased expressions of M1 and M3 muscarinic acetylcholine receptors.
The expression of beta3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.
Title: The expression of β3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.
The ventricular myocardium of rat pups differs from that of mature rats by a far greater sensitivity to selective stimulation of M3 receptors, suggesting an important functional role of M3 receptors in the newborn rats, which declines with age.
Title: Different Myocardial Sensitivity in Newborn and Mature Rats to Selective Stimulation of M3 Cholinoreceptors.
Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats.
Title: Cortical cholinergic dysregulation as a long-term consequence of neonatal hypoglycemia.
Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis.
Title: Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.

Submit: New GeneRIF Correction See all GeneRIFs (55)

General gene information

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Markers

D17Arb7 (e-PCR), detects polymorphism
- UniSTS:226819

D17Mit4 (e-PCR), detects polymorphism
- UniSTS:119317

D17Wox1 (e-PCR)
- UniSTS:118060

D17Wox12 (e-PCR)
- UniSTS:118067

D17Wox13 (e-PCR)
- UniSTS:118128

D17Mit4 (e-PCR), detects polymorphism
- UniSTS:239798

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein-coupled acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled acetylcholine receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled acetylcholine receptor binding	IDA Inferred from Direct Assay more info	PubMed
enables acetylcholine binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables quaternary ammonium group binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled acetylcholine receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in G protein-coupled acetylcholine receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in ion channel modulating, G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in ligand-gated ion channel signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of heart rate by acetylcholine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vasoconstriction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of monoatomic ion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of smooth muscle contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to acetylcholine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within saliva secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within smooth muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synaptic transmission, cholinergic	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in asymmetric synapse	IDA Inferred from Direct Assay more info	PubMed
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: muscarinic acetylcholine receptor M3

Names: cholinergic receptor, muscarinic 3, cardiac

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012527.2 → NP_036659.2 muscarinic acetylcholine receptor M3

See identical proteins and their annotated locations for NP_036659.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000017

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000080306.1, ENSRNOT00000118676.2

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 513

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086035.1 Reference GRCr8

Range

64696549..65158622

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063276047.1 → XP_063132117.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24
XM_063276048.1 → XP_063132118.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24
XM_039095336.2 → XP_038951264.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000086603.1, ENSRNOT00000107962.2

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 513

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_063276043.1 → XP_063132113.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000110565.1, ENSRNOT00000160680.1
XM_063276044.1 → XP_063132114.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000090433.1, ENSRNOT00000103154.2
XM_017600449.3 → XP_017455938.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000090085.1, ENSRNOT00000102670.2

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 513

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_063276046.1 → XP_063132116.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24
XM_063276045.1 → XP_063132115.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Related

ENSRNOP00000087869.1, ENSRNOT00000113544.2
XM_039095337.2 → XP_038951265.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 513

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_039095338.2 → XP_038951266.1 muscarinic acetylcholine receptor M3 isoform X1

UniProtKB/Swiss-Prot

P08483, Q9QWK9

UniProtKB/TrEMBL

A6KN24

Conserved Domains (3) summary

cd15299
Location:65 → 269

7tmA_mAChR_M3; muscarinic acetylcholine receptor subtype M3, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:486 → 513

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:483 → 563

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily