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    Pm20d1 peptidase M20 domain containing 1 [ Mus musculus (house mouse) ]

    Gene ID: 212933, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Pm20d1provided by MGI
    Official Full Name
    peptidase M20 domain containing 1provided by MGI
    Primary source
    MGI:MGI:2442939
    See related
    Ensembl:ENSMUSG00000042251 AllianceGenome:MGI:2442939
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    4732466D17Rik
    Summary
    Enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides. Involved in several processes, including adaptive thermogenesis; amide catabolic process; and energy homeostasis. Located in extracellular space. Is expressed in embryo; embryo endoderm; and notochord. Orthologous to human PM20D1 (peptidase M20 domain containing 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in liver adult (RPKM 26.9), large intestine adult (RPKM 13.8) and 10 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pm20d1 in Genome Data Viewer
    Location:
    1 E4; 1 57.19 cM
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (131725122..131749210)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (131797385..131821472)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 30104 Neighboring gene solute carrier family 26, member 9 Neighboring gene STARR-seq mESC enhancer starr_02224 Neighboring gene STARR-seq mESC enhancer starr_02225 Neighboring gene STARR-positive B cell enhancer ABC_E8960 Neighboring gene solute carrier family 41, member 1 Neighboring gene STARR-positive B cell enhancer ABC_E5799 Neighboring gene RAB29, member RAS oncogene family

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception. Long JZ, et al. Proc Natl Acad Sci U S A, 2018 Jul 17. PMID 29967167, Free PMC Article
    2. Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation. Song N, et al. Redox Biol, 2021 Nov. PMID 34673451, Free PMC Article
    3. A Plasma Protein Network Regulates PM20D1 and N-Acyl Amino Acid Bioactivity. Kim JT, et al. Cell Chem Biol, 2020 Sep 17. PMID 32402239, Free PMC Article
    4. Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice. Li D, et al. Eur J Pharmacol, 2019 Nov 15. PMID 31568785
    5. The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria. Long JZ, et al. Cell, 2016 Jul 14. PMID 27374330, Free PMC Article

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation.
      Title: Kir6.2 is essential to maintain neurite features by modulating PM20D1-reduced mitochondrial ATP generation.
    2. Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice
      Title: Inhibition of miR-324-5p increases PM20D1-mediated white and brown adipose loss and reduces body weight in juvenile mice.
    3. These data demonstrate that PM20D1 is a dominant enzymatic regulator of NAA levels in vivo and elucidate physiologic functions for NAA signaling in metabolism and nociception.
      Title: Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception.
    4. Study demonstrates that PM20D1 is a bidirectional enzyme in vitro, catalyzing both the condensation of fatty acids and amino acids to generate N-acyl amino acids and also the reverse hydrolytic reaction. N-acyl amino acids directly bind mitochondria and function as endogenous uncouplers of UCP1-independent respiration.
      Title: The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (5)  1 citation
    • Targeted (1) 

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Clone Names

    • MGC159098

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables aminoacylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in adaptive thermogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in amide biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in amide biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in amide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in amide catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in amide catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in amide catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in amino acid metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in amino acid metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in amino acid metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in energy homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in fatty acid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lipid metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lipid metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in respiratory electron transport chain IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in extracellular space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    N-fatty-acyl-amino acid synthase/hydrolase PM20D1
    Names
    peptidase M20 domain-containing protein 1
    probable carboxypeptidase PM20D1
    NP_001344407.1
    NP_835180.2
    XP_006529402.1
    XP_011246269.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001357478.1 → NP_001344407.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 precursor

      Status: VALIDATED

      Source sequence(s)
      AC161805, AK028883
      Consensus CDS
      CCDS15274.1
      UniProtKB/Swiss-Prot
      Q3UNX0, Q8C165, Q8R117
      Related
      ENSMUSP00000046079.6, ENSMUST00000048660.12
      Conserved Domains (1) summary
      cd05674
      Location:53 → 488
      M20_yscS; M20 Peptidase, carboxypeptidase yscS

    2. NM_178079.3 → NP_835180.2  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 precursor

      See identical proteins and their annotated locations for NP_835180.2

      Status: VALIDATED

      Source sequence(s)
      AC161805
      Consensus CDS
      CCDS15274.1
      UniProtKB/Swiss-Prot
      Q3UNX0, Q8C165, Q8R117
      Related
      ENSMUSP00000108012.3, ENSMUST00000112393.9
      Conserved Domains (1) summary
      cd05674
      Location:53 → 488
      M20_yscS; M20 Peptidase, carboxypeptidase yscS

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      131725122..131749210
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011247967.4 → XP_011246269.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 isoform X1

      Conserved Domains (1) summary
      cd05674
      Location:53 → 463
      M20_yscS; M20 Peptidase, carboxypeptidase yscS

    2. XM_006529339.2 → XP_006529402.1  N-fatty-acyl-amino acid synthase/hydrolase PM20D1 isoform X2

      Conserved Domains (1) summary
      cd05674
      Location:53 → 463
      M20_yscS; M20 Peptidase, carboxypeptidase yscS
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8C165.1 GenPept UniProtKB/Swiss-Prot:Q8C165

    Gene LinkOut

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