Gnb5 guanine nucleotide binding protein (G protein), beta 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gnb5provided by MGI
Official Full Name: guanine nucleotide binding protein (G protein), beta 5provided by MGI
Primary source: MGI:MGI:101848
See related: Ensembl:ENSMUSG00000032192 AllianceGenome:MGI:101848
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GBS; flr; Hg2e; Gbeta5
Summary: Predicted to enable several functions, including G-protein gamma-subunit binding activity; GTPase activating protein binding activity; and protein-folding chaperone binding activity. Predicted to contribute to GTPase activator activity. Acts upstream of or within G protein-coupled receptor signaling pathway; dark adaptation; and light adaption. Located in nucleus and plasma membrane. Is active in several cellular components, including dendrite; parallel fiber to Purkinje cell synapse; and synaptic membrane. Is expressed in several structures, including brain; eye; genitourinary system; liver; and lung. Human ortholog(s) of this gene implicated in intellectual developmental disorder with cardiac arrhythmia. Orthologous to human GNB5 (G protein subunit beta 5). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in cerebellum adult (RPKM 36.1), frontal lobe adult (RPKM 31.3) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 D; 9 42.3 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (75213570..75253205)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (75306288..75345923)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Specific regulation of mechanical nociception by Gbeta5 involves GABA-B receptors.
Title: Specific regulation of mechanical nociception by Gβ5 involves GABA-B receptors.
The regulatory G protein signaling complex, Gbeta5-R7, promotes glucose- and extracellular signal-stimulated insulin secretion.
Title: The regulatory G protein signaling complex, Gβ5-R7, promotes glucose- and extracellular signal-stimulated insulin secretion.
Stimulation of the ERK pathway in Gnb5(-/-) cells by epidermal growth factor restored M3R-stimulated insulin release to near normal levels. Identification of the novel role of Gbeta5-R7 in insulin secretion may lead to a new therapeutic approach for improving pancreatic beta-cell function
Title: Regulator of G-protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion.
The complex of G protein regulator RGS9-2 and Gbeta(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum.
Title: The complex of G protein regulator RGS9-2 and Gβ(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum.
Expression of the Gbeta5/R7-RGS protein complex in pituitary and pancreatic islet cells.
Title: Expression of the Gβ5/R7-RGS protein complex in pituitary and pancreatic islet cells.
Gbeta5/RGS6 (guanine nucleotide binding protein beta5/regulator of G-protein signaling 6) complex modulates timing of parasympathetic influence on atrial myocytes and heart rate.
Title: RGS6/Gβ5 complex accelerates IKACh gating kinetics in atrial myocytes and modulates parasympathetic regulation of heart rate.
Elimination of Gbeta5-RGS complexes led to a striking level of hyperactivity that far exceeds activity levels previously observed in animal models.
Title: Gβ5-RGS complexes are gatekeepers of hyperactivity involved in control of multiple neurotransmitter systems.
We conclude that even a partial reduction in Gbeta5-R7 level can perturb normal animal metabolism and behavior.
Title: Targeted deletion of one or two copies of the G protein β subunit Gβ5 gene has distinct effects on body weight and behavior in mice.
Gbeta5 regulates dendritic arborization and/or synapse formation during development, in part by effects on gene expression; mice lacking Gbeta5 have a markedly abnormal neurologic phenotype.
Title: Knockout of G protein β5 impairs brain development and causes multiple neurologic abnormalities in mice.
regulator of G-protein signalling 7 binding protein contributes significantly to the nuclear localization of endogenous G beta(5)/R7-RGS complex in brain
Title: Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Gnb5 (e-PCR), detects polymorphism
- UniSTS:496684

Gnb5 (e-PCR), detects polymorphism
- UniSTS:164922

NoName (e-PCR)
- UniSTS:224331

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G-protein gamma-subunit binding	ISO Inferred from Sequence Orthology more info
enables G-protein gamma-subunit binding	ISS Inferred from Sequence or Structural Similarity more info
enables GTPase activating protein binding	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
contributes_to GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-folding chaperone binding	ISO Inferred from Sequence Orthology more info
enables protein-folding chaperone binding	ISS Inferred from Sequence or Structural Similarity more info
enables signaling receptor complex adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in G protein-coupled dopamine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled dopamine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in G protein-coupled dopamine receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within dark adaptation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within light adaption	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of voltage-gated calcium channel activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of voltage-gated calcium channel activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of GTPase activity	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell body	ISO Inferred from Sequence Orthology more info
is_active_in cell tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in dendrite	IDA Inferred from Direct Assay more info	PubMed
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
located_in myelin sheath	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in parallel fiber to Purkinje cell synapse	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	EXP Inferred from Experiment more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: guanine nucleotide-binding protein subunit beta-5

Names: flail; flailer; guanine nucleotide binding protein, beta 5; heterotrimeric guanine nucleotide-binding protein 2e; hug; transducin beta chain 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010313.2 → NP_034443.1 guanine nucleotide-binding protein subunit beta-5 isoform 1

See identical proteins and their annotated locations for NP_034443.1

Status: VALIDATED

Description

Transcript Variant: This variant (1; also known as Long, Beta-5L, Gbeta5L) encodes the longer isoform (1).

Source sequence(s)

AI323394, BX513135, BY607717, CK621143, U69145

Consensus CDS

CCDS40692.1

UniProtKB/Swiss-Prot

O35354, P54314, P62881, Q91WB3

UniProtKB/TrEMBL

Q3V331

Related

ENSMUSP00000149938.2, ENSMUST00000213990.2

Conserved Domains (2) summary

cd00200
Location:98 → 395

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:108 → 145

7WD40; WD40 repeat [structural motif]
NM_138719.5 → NP_619733.1 guanine nucleotide-binding protein subunit beta-5 isoform 2

See identical proteins and their annotated locations for NP_619733.1

Status: VALIDATED

Description

Transcript Variant: This variant (2; also known as Gbeta5S) differs in the 5' UTR compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC133947

Consensus CDS

CCDS90626.1

UniProtKB/TrEMBL

Q3UG14, Q3V331

Related

ENSMUSP00000150492.2, ENSMUST00000215875.2

Conserved Domains (2) summary

cd00200
Location:56 → 353

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:66 → 103

7WD40; WD40 repeat [structural motif]

RNA

NR_073186.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate 5' exon structure and contains an alternate internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC133947