Prkaa2 protein kinase, AMP-activated, alpha 2 catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkaa2provided by MGI
Official Full Name: protein kinase, AMP-activated, alpha 2 catalytic subunitprovided by MGI
Primary source: MGI:MGI:1336173
See related: Ensembl:ENSMUSG00000028518 AllianceGenome:MGI:1336173
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: A830082D05; AMPKalpha2; 2310008I11Rik
Summary: Enables AMP-activated protein kinase activity; chromatin binding activity; and histone H2BS36 kinase activity. Involved in several processes, including regulation of autophagy; regulation of organelle organization; and regulation of protein modification process. Acts upstream of or within several processes, including cellular response to prostaglandin E stimulus; cellular response to xenobiotic stimulus; and response to muscle activity. Located in several cellular components, including cytoplasmic stress granule; dendrite; and neuronal cell body. Is expressed in several structures, including brain; genitourinary system; gut; lung; and spinal cord. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human PRKAA2 (protein kinase AMP-activated catalytic subunit alpha 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in heart adult (RPKM 10.8), kidney adult (RPKM 9.5) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 C6; 4 49.05 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (104886846..104967095, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (105029649..105109898, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging.
Title: Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging.
Skeletal muscle-specific inducible AMPKalpha1/alpha2 knockout mice develop muscle weakness, glycogen depletion, and fibrosis that persists during disuse atrophy.
Title: Skeletal muscle-specific inducible AMPKα1/α2 knockout mice develop muscle weakness, glycogen depletion, and fibrosis that persists during disuse atrophy.
AMPKalpha2 regulates fasting-induced hyperketonemia by suppressing SCOT ubiquitination and degradation.
Title: AMPKα2 regulates fasting-induced hyperketonemia by suppressing SCOT ubiquitination and degradation.
ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA.
Title: ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA.
Quantification and Proteomic Characterization of beta-Hydroxybutyrylation Modification in the Hearts of AMPKalpha2 Knockout Mice.
Title: Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice.
AMP-activated protein kinase alpha2 contributes to acute and chronic hyperuricemic nephropathy via renal urate deposition in a mouse model.
Title: AMP-activated protein kinase α2 contributes to acute and chronic hyperuricemic nephropathy via renal urate deposition in a mouse model.
Conditional disruption of AMP kinase in dopaminergic neurons promotes Parkinson's disease-associated phenotypes in vivo.
Title: Conditional disruption of AMP kinase in dopaminergic neurons promotes Parkinson's disease-associated phenotypes in vivo.
Deletion of AMPK minimizes graft-versus-host disease through an early impact on effector donor T cells.
Title: Deletion of AMPK minimizes graft-versus-host disease through an early impact on effector donor T cells.
Protein expression alteration in hippocampus upon genetic repression of AMPKalpha isoforms.
Title: Protein expression alteration in hippocampus upon genetic repression of AMPKα isoforms.
Roles of KLF4 and AMPK in the inhibition of glycolysis by pulsatile shear stress in endothelial cells.
Title: Roles of KLF4 and AMPK in the inhibition of glycolysis by pulsatile shear stress in endothelial cells.

Submit: New GeneRIF Correction See all GeneRIFs (169)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables AMP-activated protein kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables AMP-activated protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables AMP-activated protein kinase activity	ISO Inferred from Sequence Orthology more info
enables AMP-activated protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info
enables protein serine/threonine/tyrosine kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in cellular response to calcium ion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to glucose starvation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose stimulus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nutrient levels	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to nutrient levels	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nutrient levels	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to oxidative stress	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to prostaglandin E stimulus	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in energy homeostasis	ISO Inferred from Sequence Orthology more info
involved_in energy homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in fatty acid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in fatty acid homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in glucose homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid droplet disassembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_positive_effect lipid droplet disassembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of TOR signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hepatocyte apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of hepatocyte apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of tubulin deacetylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glycolytic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of peptidyl-lysine acetylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to lipid droplet	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to lipid droplet	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of macroautophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of stress granule assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to activity	ISO Inferred from Sequence Orthology more info
involved_in response to caffeine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to muscle activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in axon	IGI Inferred from Genetic Interaction more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	IGI Inferred from Genetic Interaction more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IGI Inferred from Genetic Interaction more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
part_of nucleotide-activated protein kinase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 5'-AMP-activated protein kinase catalytic subunit alpha-2

Names: ACACA kinase; AMPK subunit alpha-2; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase

NP_001343497.1

EC 2.7.11.1

EC 2.7.11.31

NP_835279.2

EC 2.7.11.1

EC 2.7.11.31

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001356568.1 → NP_001343497.1 5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform 2

Status: VALIDATED

Source sequence(s)

AK009231, AK020297, AK044030, AL627307, AL929466, CJ283122, CK618328, CX563939

Conserved Domains (3) summary

cd14404
Location:195 → 259

UBA_AID_AAPK2; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPKalpha-2)

cl17070
Location:305 → 460

AMPKA_C_like; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha subunit and similar domains

cl21453
Location:1 → 178

PKc_like; Protein Kinases, catalytic domain
NM_178143.2 → NP_835279.2 5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform 1

See identical proteins and their annotated locations for NP_835279.2

Status: VALIDATED

Source sequence(s)

AL627307, AL929466, BC138565

Consensus CDS

CCDS18416.1

UniProtKB/Swiss-Prot

B1ASQ8, Q3UYM4, Q8BRK8

Related

ENSMUSP00000030243.8, ENSMUST00000030243.8

Conserved Domains (3) summary

cd14404
Location:285 → 349

UBA_AID_AAPK2; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPKalpha-2)

cd14079
Location:13 → 268

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase

cl17070
Location:395 → 550

AMPKA_C_like; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha subunit and similar domains