Dgkg diacylglycerol kinase, gamma [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkgprovided by MGI
Official Full Name: diacylglycerol kinase, gammaprovided by MGI
Primary source: MGI:MGI:105060
See related: Ensembl:ENSMUSG00000022861 AllianceGenome:MGI:105060
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 90kDa; Dagk3; mKIAA4131; 2900055E17Rik; E430001K23Rik
Summary: Enables ATP-dependent diacylglycerol kinase activity. Involved in several processes, including glycerolipid metabolic process; lipid phosphorylation; and negative regulation of phospholipase C/protein kinase C signal transduction. Predicted to be located in cytosol and membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; integumental system; and sensory organ. Orthologous to human DGKG (diacylglycerol kinase gamma). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in cerebellum adult (RPKM 10.5), frontal lobe adult (RPKM 4.6) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 B1; 16 13.37 cM

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (22285318..22476051, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (22466568..22657302, complement)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
Title: Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
DGKgamma Knock-Out Mice Show Impairments in Cerebellar Motor Coordination, LTD, and the Dendritic Development of Purkinje Cells through the Activation of PKCgamma.
Title: DGKγ Knock-Out Mice Show Impairments in Cerebellar Motor Coordination, LTD, and the Dendritic Development of Purkinje Cells through the Activation of PKCγ.
Precise Regulation of the Basal PKCgamma Activity by DGKgamma Is Crucial for Motor Coordination.
Title: Precise Regulation of the Basal PKCγ Activity by DGKγ Is Crucial for Motor Coordination.
A novel morphological function of the C-terminal DGK-gamma catalytic region in filopodia formation, independent of its enzymatic activity.
Title: Induction of filopodia-like protrusions in N1E-115 neuroblastoma cells by diacylglycerol kinase γ independent of its enzymatic activity: potential novel function of the C-terminal region containing the catalytic domain of diacylglycerol kinase γ.
DGKgamma functions through its catalytic action as an upstream suppressor of Rac1 and has a role in lamellipodium/ruffle formation
Title: Diacylglycerol kinase gamma serves as an upstream suppressor of Rac1 and lamellipodium formation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126781 (e-PCR)
- UniSTS:209190

AI854428 (e-PCR)
- UniSTS:165985

UniSTS:237466 (e-PCR)
- UniSTS:237466

D16Mit98 (e-PCR), detects polymorphism
- UniSTS:127935

D16Mit98.2 (e-PCR), detects polymorphism
- UniSTS:127936

Dgkg (e-PCR), detects polymorphism
- UniSTS:506845

fk56e12.x1 (e-PCR)
- UniSTS:201209

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables lipid binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of phospholipase C/protein kinase C signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron development	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: diacylglycerol kinase gamma

Names: 88 kDa diacylglycerol kinase; DAG kinase gamma; DGK-gamma; diacylglycerol kinase, gamma 3; diacylglycerol kinase, gamma 90kDa; diglyceride kinase gamma

NP_001333602.1

EC 2.7.1.107

NP_619591.1

EC 2.7.1.107

XP_017172339.1

EC 2.7.1.107

XP_017172340.1

EC 2.7.1.107

XP_017172341.1

EC 2.7.1.107

XP_036015627.1

EC 2.7.1.107

XP_036015628.1

EC 2.7.1.107

XP_036015629.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001346673.1 → NP_001333602.1 diacylglycerol kinase gamma isoform 2

Status: VALIDATED

Source sequence(s)

AI646503, AK083258, BB653405, BY724037, CT009627

Consensus CDS

CCDS84217.1

UniProtKB/TrEMBL

Q570Z5, Q8C413

Related

ENSMUSP00000023578.8, ENSMUST00000023578.14
NM_138650.2 → NP_619591.1 diacylglycerol kinase gamma isoform 1

See identical proteins and their annotated locations for NP_619591.1

Status: VALIDATED

Source sequence(s)

AK013697, AK220294, BU700613, BY724037

Consensus CDS

CCDS28068.1

UniProtKB/Swiss-Prot

Q91WG7

UniProtKB/TrEMBL

Q570Z5

Related

ENSMUSP00000087371.4, ENSMUST00000089925.10

Conserved Domains (8) summary

TIGR00147
Location:433 → 753

TIGR00147; lipid kinase, YegS/Rv2252/BmrU family

smart00109
Location:333 → 374

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:575 → 749

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:433 → 554

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:269 → 318

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:176 → 245

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:177 → 241

EF-hand_7; EF-hand domain pair

pfam14513
Location:5 → 172

DAG_kinase_N; Diacylglycerol kinase N-terminus

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000082.7 Reference GRCm39 C57BL/6J

Range

22285318..22476051 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316851.3 → XP_017172340.1 diacylglycerol kinase gamma isoform X2

UniProtKB/TrEMBL

Q570Z5

Conserved Domains (5) summary

smart00045
Location:582 → 756

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:440 → 561

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:315 → 364

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:176 → 291

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:5 → 172

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_017316850.3 → XP_017172339.1 diacylglycerol kinase gamma isoform X1

UniProtKB/TrEMBL

Q570Z5

Conserved Domains (6) summary

smart00109
Location:379 → 420

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:621 → 795

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:479 → 600

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:315 → 364

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:176 → 291

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:5 → 172

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_036159736.1 → XP_036015629.1 diacylglycerol kinase gamma isoform X6

Conserved Domains (4) summary

smart00045
Location:340 → 514

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:198 → 319

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cl00040
Location:56 → 129

C1; protein kinase C conserved region 1 (C1 domain) superfamily

cl08302
Location:15 → 49

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
XM_036159735.1 → XP_036015628.1 diacylglycerol kinase gamma isoform X5

Conserved Domains (4) summary

smart00045
Location:379 → 553

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:237 → 358

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cl00040
Location:56 → 129

C1; protein kinase C conserved region 1 (C1 domain) superfamily

cl08302
Location:15 → 49

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
XM_036159734.1 → XP_036015627.1 diacylglycerol kinase gamma isoform X4

Conserved Domains (4) summary

smart00045
Location:425 → 599

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:283 → 404

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cl00040
Location:102 → 175

C1; protein kinase C conserved region 1 (C1 domain) superfamily

cl08302
Location:64 → 95

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
XM_017316852.3 → XP_017172341.1 diacylglycerol kinase gamma isoform X3

UniProtKB/TrEMBL

Q570Z5

Conserved Domains (6) summary

smart00109
Location:379 → 420

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00046
Location:479 → 600

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:315 → 364

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:176 → 291

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:5 → 172

DAG_kinase_N; Diacylglycerol kinase N-terminus

cl02440
Location:621 → 743

DAGK_acc; Diacylglycerol kinase accessory domain