Pcyt1a phosphate cytidylyltransferase 1A, choline [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pcyt1aprovided by RGD
Official Full Name: phosphate cytidylyltransferase 1A, cholineprovided by RGD
Primary source: RGD:70515
See related: EnsemblRapid:ENSRNOG00000001762 AllianceGenome:RGD:70515
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CTP; CCT-alpha
Summary: Enables several functions, including calmodulin binding activity; choline-phosphate cytidylyltransferase activity; and phosphatidylcholine binding activity. Involved in CDP-choline pathway. Located in nuclear envelope. Human ortholog(s) of this gene implicated in congenital generalized lipodystrophy and spondylometaphyseal dysplasia with cone-rod dystrophy. Orthologous to human PCYT1A (phosphate cytidylyltransferase 1A, choline). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Heart (RPKM 257.0), Muscle (RPKM 202.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 11q22

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	11	NC_086029.1 (81818914..81862623, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	11	NC_051346.1 (68313860..68357828, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	11	NC_005110.4 (71547865..71592037, complement)

Chromosome 11 - NC_086029.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that exogenous prostaglandin D2 (PGD2) induces CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) distribution to nuclear envelope from intranuclear Lamin A/C foci.
Title: Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells.
This work deciphers how the CCT regulatory amphipathic helix functions as a silencing device.
Title: Structural basis for autoinhibition of CTP:phosphocholine cytidylyltransferase (CCT), the regulatory enzyme in phosphatidylcholine synthesis, by its membrane-binding amphipathic helix.
ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase alpha contributes to malignant transformation of intestinal epithelial cells
Title: ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.
the 22-mer segment in the structurally pliable regulatory domain of rat CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions
Title: A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions.
7beta-estradiol can increase CTP: phosphorylcholine cytidylyltransferase activity in cultured lung explants.
Title: [Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants].
127 fibroblasts was heterogeneous, with evidence of nucleoplasmic, cytoplasmic and NE staining.
Title: CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis.
The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline.
Title: Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
translocation and activation of CCTalpha at nuclear membranes by side-chain hydroxylated sterols are regulated by the cholesterol status of the cell
Title: Oxysterol activation of phosphatidylcholine synthesis involves CTP:phosphocholine cytidylyltransferase alpha translocation to the nuclear envelope.
Data show that anionic lipids, working via surface-localized pH effects, can promote membrane binding by modifying protein charge and hydrophobicity, which contributes to the membrane selectivity of CTP:phosphocholine cytidylyltransferase in vivo.
Title: Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranes.
Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase
Title: Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Markers

STS-L28957 (e-PCR)
- UniSTS:78865

RH130304 (e-PCR)
- UniSTS:213589

D11Rat106 (e-PCR), detects polymorphism
- UniSTS:226399

BE108960 (e-PCR)
- UniSTS:247931

Pcyt1a (e-PCR), detects polymorphism
- UniSTS:259504

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables choline-phosphate cytidylyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline-phosphate cytidylyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables choline-phosphate cytidylyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables choline-phosphate cytidylyltransferase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	EXP Inferred from Experiment more info	PubMed
enables lipid binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables molecular function inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylcholine binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylcholine binding	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in B cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in B cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in CDP-choline pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in CDP-choline pathway	ISO Inferred from Sequence Orthology more info
involved_in isotype switching	ISO Inferred from Sequence Orthology more info
involved_in isotype switching	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphatidylcholine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylcholine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylcholine biosynthetic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in extrinsic component of membrane	NAS Non-traceable Author Statement more info	PubMed
located_in glycogen granule	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: choline-phosphate cytidylyltransferase A

Names: CCT A; CT A; CTP:phosphocholine cytidylyl transferase; CTP:phosphocholine cytidylyltransferase A; CTP:phosphocholine cytidylyltransferase alpha; choline phosphate cytidylyltransferase 1 alpha; choline-phosphate cytidylyltransferase A-like; phosphate cytidylyltransferase 1, choline, alpha; phosphorylcholine transferase A

NP_511177.2

EC 2.7.7.15

XP_006248506.1

EC 2.7.7.15

XP_008766916.1

EC 2.7.7.15

XP_038943847.1

EC 2.7.7.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_078622.2 → NP_511177.2 choline-phosphate cytidylyltransferase A

See identical proteins and their annotated locations for NP_511177.2

Status: VALIDATED

Source sequence(s)

AW919077, BF285251, BQ780519, L13245

UniProtKB/Swiss-Prot

P19836

UniProtKB/TrEMBL

A6IRR3, A6IRR5

Related

ENSRNOP00000002403.3, ENSRNOT00000002403.5

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086029.1 Reference GRCr8

Range

81818914..81862623 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008768694.4 → XP_008766916.1 choline-phosphate cytidylyltransferase A isoform X1

See identical proteins and their annotated locations for XP_008766916.1

UniProtKB/Swiss-Prot

P19836

UniProtKB/TrEMBL

A6IRR3, A6IRR5

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase
XM_006248444.5 → XP_006248506.1 choline-phosphate cytidylyltransferase A isoform X1

See identical proteins and their annotated locations for XP_006248506.1

UniProtKB/Swiss-Prot

P19836

UniProtKB/TrEMBL

A6IRR3, A6IRR5

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase
XM_039087919.2 → XP_038943847.1 choline-phosphate cytidylyltransferase A isoform X1

UniProtKB/Swiss-Prot

P19836

UniProtKB/TrEMBL

A6IRR3, A6IRR5

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase