loqs loquacious [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: loqsprovided by FlyBase
Official Full Name: loquaciousprovided by FlyBase
Primary source: FLYBASE:FBgn0032515
Locus tag: Dmel_CG6866
See related: AllianceGenome:FB:FBgn0032515
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cg6866; CG6866; Dmel\CG6866; dRax; loq; Loqs; LOQS; Loqs-PD; LqPD; r3d1; R3D1; R3D1-L; R3D1-S; TRBP
Summary: Enables RISC complex binding activity and siRNA binding activity. Involved in several processes, including female germ-line stem cell asymmetric division; miRNA metabolic process; and regulation of gene expression. Located in cytosol. Part of RISC-loading complex. Is expressed in several structures, including CNS glial cell; adult head; embryonic/larval midgut primordium; ganglia; and mesoderm. Human ortholog(s) of this gene implicated in dystonia 16. Orthologous to several human genes including TARBP2 (TARBP2 subunit of RISC loading complex). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 34B9-34B9; 2-48 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (13382652..13385671)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (13382652..13385671)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Title: Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Title: Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Loquacious-PD regulates the terminus-dependent molecular recognition of Dicer-2 toward double-stranded RNA.
Title: Loquacious-PD regulates the terminus-dependent molecular recognition of Dicer-2 toward double-stranded RNA.
We also found that a base-mismatch can enable Loqs-PB to tune miRNA length. We speculate that Loqs-PB tunes miRNA length by changing the conformation of base-mismatched nucleotides in the pre-miRNA stem to that of base-matched ones.
Title: Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem.
studies indicate that the 22 amino acids at the C terminus of Loqs-PD, including an FDF-like motif, directly interact with the Hel2 subdomain of Dicer-2's helicase domain. This interaction is RNA-independent, but we find that modulation of Dicer-2 cleavage also requires dsRNA binding by Loqs-PD.
Title: Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA.
Loqs-PD removes the inhibitory effect of inorganic phosphate on Dicer-2 processing of blunt-end hairpin RNAs, allowing siRNA production in the presence of inorganic phosphate.
Title: Loquacious-PD removes phosphate inhibition of Dicer-2 processing of hairpin RNAs into siRNAs.
binding preference for the thermodynamically more stable siRNA end by Loqs-PD alone is greatly amplified in complex with Dcr-2 to initiate strand discrimination by asymmetry sensing in the RISC loading complex.
Title: Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.
Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates.
Title: The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site.
Partial reconstitution of the RNAi response in human cells has been demonstrated using Drosophila gene products, DCR-2, Loqs-PD, and R2D2.
Title: Partial reconstitution of the RNAi response in human cells using Drosophila gene products.
Loqs binds to dmDcr1 as a monomer using the identified dimerization surface, which suggests that Loqs might form dimers under conditions where dmDcr1 is absent or not accessible
Title: Structural and functional insights into the fly microRNA biogenesis factor Loquacious.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RISC complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables double-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dosage compensation by hyperactivation of X chromosome	IGI Inferred from Genetic Interaction more info	PubMed
involved_in female germ-line stem cell asymmetric division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-line stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of regulatory ncRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of regulatory ncRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC-loading complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC-loading complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: loquacious

Names: CG6866-PA; CG6866-PB; CG6866-PC; CG6866-PD; CG6866-PE; CG6866-PF; loqs-PA; loqs-PB; loqs-PC; loqs-PD; loqs-PE; loqs-PF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

13382652..13385671

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001038814.3 → NP_001033903.1 loquacious, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001033903.1

Status: REVIEWED

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_135802.4 → NP_609646.1 loquacious, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_609646.1

Status: REVIEWED

UniProtKB/Swiss-Prot

M9MRT5, Q4TZM6, Q8IP72, Q8SZ74, Q9VJY9

Related

FBpp0080076

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001298965.1 → NP_001285894.1 loquacious, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285894.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J5X6

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_165041.3 → NP_723813.1 loquacious, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723813.1

Status: REVIEWED

Related

FBpp0080075

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001298964.1 → NP_001285893.1 loquacious, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285893.1

Status: REVIEWED

UniProtKB/TrEMBL

X2JAB1

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001201867.2 → NP_001188796.1 loquacious, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001188796.1

Status: REVIEWED

Conserved Domains (2) summary

smart00358
Location:136 → 204

DSRM; Double-stranded RNA binding motif

cd00048
Location:250 → 316

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...