Klp61F Kinesin-like protein at 61F [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Klp61Fprovided by FlyBase
Official Full Name: Kinesin-like protein at 61Fprovided by FlyBase
Primary source: FLYBASE:FBgn0004378
Locus tag: Dmel_CG9191
See related: AllianceGenome:FB:FBgn0004378
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 6345; CG9191; CG9191-PA; Dmel\CG9191; DmKlp61F; DmKLP61F; DmKRP130; KIF 8; KIF11; Kin-5; Kin5; Klp 61F; Klp2; KLP2; KLP61; klp61f; klp61F; Klp61f; KLP61f; KLP61F; Klp61F/KRP130; Krp130; KRP130; KRP[[(130)]]; KRP[[130]]; l(3)07012; l(3)6D283; l(3)Klp61F; urc; urcn
Summary: Enables microtubule binding activity and plus-end-directed microtubule motor activity. Involved in several processes, including microtubule cytoskeleton organization; plus-end directed microtubule sliding; and positive regulation of Golgi to plasma membrane protein transport. Located in several cellular components, including Golgi apparatus; fusome; and spindle. Part of kinesin complex. Colocalizes with centrosome. Is expressed in several structures, including CNS glial cell; gonad; head epidermis primordium; sensory nervous system primordium; and ventral nerve cord. Used to study microcephaly with or without chorioretinopathy, lymphedema, or mental retardation. Human ortholog(s) of this gene implicated in microcephaly with or without chorioretinopathy, lymphedema, or mental retardation. Orthologous to human KIF11 (kinesin family member 11). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 61F4-61F4; 3-0 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (1241568..1245910, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (1241568..1245910, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity.
Title: Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity.
Kinesin-5 stabilizes the microtubule network during interphase in syncytial embryos.
Title: A 'molecular guillotine' reveals the interphase function of Kinesin-5.
KLP61F is required for spindle and centromere symmetry in oocytes.
Title: Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes.
Kinesin-5 pauses at microtubule plus ends and enhances polymerization by stabilizing longitudinal tubulin-tubulin interactions. These activities underlie the ability of kinesin-5 to slide and stabilize microtubule bundles in cells.
Title: Kinesin-5 is a microtubule polymerase.
Klp61F knockdown inhibits post-Golgi transport in Drosophila S2 cells.
Title: Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.
A staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments.
Title: The bipolar assembly domain of the mitotic motor kinesin-5.
Tyrosines in the kinesin-5 (KLP61F)head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.
Title: Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.
The work tends to support the "sliding filament" mechanism for KLP61F action in Drosophila embryo spindles.
Title: Kinesin-5 in Drosophila embryo mitosis: sliding filament or spindle matrix mechanism?
calculated the structure of a K5 motor bound to MTs at 9 A resolution, providing insight into this important interaction.
Title: 9-Angström structure of a microtubule-bound mitotic motor.
KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates
Title: Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables plus-end-directed microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables plus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fusome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule bundle formation	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	HMP more info	PubMed
involved_in mitotic centrosome separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plus-end directed microtubule sliding	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Golgi to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle elongation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in aster	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in fusome	IDA Inferred from Direct Assay more info	PubMed
part_of kinesin complex	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
part_of microtubule associated complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle pole	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle microtubule	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Kinesin-like protein at 61F

Names: CG9191-PA; Kinesin-related protein-130kD; Kinesin8B; Klp61F-PA; kinesin-5; kinesin-5 motor; kinesin-5s; kinesin-like protein; urchin

NP_476818.1

EC 5.6.1.3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

1241568..1245910 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057470.5 → NP_476818.1 Kinesin-like protein at 61F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476818.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P46863, Q8T0A6, Q9W0I8

UniProtKB/TrEMBL

Q0PQ30, Q0PQ31

Related

FBpp0072616

Conserved Domains (3) summary

cd01364
Location:17 → 365

KISc_BimC_Eg5; Kinesin motor domain, BimC/Eg5 spindle pole proteins

pfam00225
Location:25 → 356

Kinesin; Kinesin motor domain

pfam13931
Location:923 → 1009

Microtub_bind; Kinesin-associated microtubule-binding