Trim36 tripartite motif-containing 36 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim36provided by MGI
Official Full Name: tripartite motif-containing 36provided by MGI
Primary source: MGI:MGI:106264
See related: Ensembl:ENSMUSG00000033949 AllianceGenome:MGI:106264
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: haprin; D18Wsu100e
Summary: Enables alpha-tubulin binding activity and ubiquitin-protein transferase activity. Involved in regulation of cell cycle. Acts upstream of or within acrosome reaction. Located in acrosomal vesicle. Is expressed in extraembryonic component; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in anencephaly. Orthologous to human TRIM36 (tripartite motif containing 36). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in testis adult (RPKM 82.8), whole brain E14.5 (RPKM 6.2) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 C; 18 24.41 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (46298367..46345674, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (46165300..46212607, complement)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Haprin-deficient spermatozoa are incapable of in vitro fertilization.
Title: Haprin-deficient spermatozoa are incapable of in vitro fertilization.
results indicated that the novel RBCC protein Haprin plays a key role in the acrosome reaction and fertilization
Title: Haprin, a novel haploid germ cell-specific RING finger protein involved in the acrosome reaction.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D18Wsu100e (e-PCR), detects polymorphism
- UniSTS:142199

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables alpha-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-protein transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in acrosome reaction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within acrosome reaction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within microtubule bundle formation	ISO Inferred from Sequence Orthology more info	PubMed
involved_in mitotic cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in mitotic cytokinesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of microtubule cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle organization	ISO Inferred from Sequence Orthology more info
involved_in spindle organization	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in acrosomal vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase Trim36

Names: RING-type E3 ubiquitin transferase TRIM36; acrosome RBCC protein; tripartite motif-containing protein 36

NP_001164326.1

EC 2.3.2.27

NP_849203.2

EC 2.3.2.27

XP_036017058.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001170855.1 → NP_001164326.1 E3 ubiquitin-protein ligase Trim36 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AC124717, BC058106, BM938922, CJ046908, CJ301781

Consensus CDS

CCDS50279.1

UniProtKB/TrEMBL

E9Q3A0

Related

ENSMUSP00000129771.2, ENSMUST00000167364.9

Conserved Domains (5) summary

smart00060
Location:419 → 482

FN3; Fibronectin type 3 domain

cd00021
Location:199 → 237

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam13445
Location:21 → 49

zf-RING_UBOX; RING-type zinc-finger

cd12894
Location:504 → 707

SPRY_PRY_TRIM36; PRY/SPRY domain in tripartite motif-containing protein 36 (TRIM36)

cl23765
Location:244 → 368

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_178872.4 → NP_849203.2 E3 ubiquitin-protein ligase Trim36 isoform 1

See identical proteins and their annotated locations for NP_849203.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

BC058106, BM938922, CJ167273, CJ301781

Consensus CDS

CCDS29229.1

UniProtKB/Swiss-Prot

Q7TNM1, Q80WG7

Related

ENSMUSP00000037978.5, ENSMUST00000037011.6

Conserved Domains (5) summary

smart00060
Location:431 → 494

FN3; Fibronectin type 3 domain

cd00021
Location:211 → 249

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam13445
Location:33 → 61

zf-RING_UBOX; RING-type zinc-finger

cd12894
Location:516 → 719

SPRY_PRY_TRIM36; PRY/SPRY domain in tripartite motif-containing protein 36 (TRIM36)

cl23765
Location:256 → 380

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

46298367..46345674 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161165.1 → XP_036017058.1 E3 ubiquitin-protein ligase Trim36 isoform X1

Conserved Domains (7) summary

smart00502
Location:167 → 291

BBC; B-Box C-terminal domain

smart00060
Location:342 → 405

FN3; Fibronectin type 3 domain

cd12894
Location:427 → 630

SPRY_PRY_TRIM36; PRY/SPRY domain in tripartite motif-containing protein 36 (TRIM36)

cd19778
Location:121 → 165

Bbox2_TRIM36_C-I; B-box-type 2 zinc finger found in tripartite motif-containing protein 36 (TRIM36) and similar proteins

cd19848
Location:62 → 116

Bbox1_TRIM36_C-I; B-box-type 1 zinc finger found in tripartite motif-containing protein 36 (TRIM36) and similar proteins

pfam18568
Location:270 → 318

COS; TRIM C-terminal subgroup One Signature domain

cl17238
Location:22 → 36

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain